Enhanced production of scopolamine in induced autotetraploid plants of Hyoscyamus reticulatus L.

• Autorzy: Madani H. , Hosseini B. , Dehghan E. , Rezaei-chiyaneh E.
• Języki publikacji: EN

Abstrakty

EN

Asimple and efficient procedure was established for in vitro propagation of lattice henbane (Hyoscyamus reticulatus L.) using shoot tip explants cultured on Murashige and Skoog (MS) medium supplemented with N6-benzylaminopurine (BAP) and indolyl-3-acetic acid (IAA). In vitro-induced autotetraploid plants of H. reticulatus L. exhibiting high yield of scopolamine were successfully induced by different concentrations of colchicine solutions (0.00, 0.05, 0.1, 0.2 and 0.5 %) which were applied for 24, 48 and 72 h. Treated shoot tips were regenerated on MS medium supplemented with 8.8 mM BAP and 2.2 mMIAA and rooted on MS medium containing 2.2 mM indolyl-3- butyric acid, then were acclimatized and transferred to soil. According to the results, 0.1 % (w/v) of colchicine for 48 h can be effective for induction of polyploidy in H. reticulatus L. The induced tetraploid plants represented a different structural, physiological and biochemical characteristics. Autotetraploid plants of H. reticulatus also showed a higher conversion of hyoscyamine to scopolamine as scopolamine content was increased from 0.23 in diploids to 8.66 % in the induced tetraploid plants. Regarding the higher commercialvalue of scopolamine than the other tropane alkaloids, tetraploidy can efficiently be used to improve scopolamine production of H. reticulatus.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 03
• Opis fizyczny: Article: 55 [7 p.], fig.,ref.

Twórcy

autor

Madani H.

• Department of Horticulture, Faculty of Agriculture, Urmia University, P.O.Box 165, Urmia, Iran

autor

Hosseini B.

• Department of Horticulture, Faculty of Agriculture, Urmia University, P.O.Box 165, Urmia, Iran

autor

Dehghan E.

• Department of Plant Biotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O.Box 9177948978, Mashhad, Iran

autor

Rezaei-chiyaneh E.

• Shahid bakeri Higher Education Center of Miandoab, Urmia University, Urmia, Iran

Bibliografia

• Ahuja A, Sambyal M, Koul S (2002) In vitro propagation and conservation of Atropa acuminata Royle ex Lindl-An indigenous threatened medicinal plant. J Plant Biochem Biotechnol 11(2):121–124
• Al-Humaid AI (2003) Effects of compound fertilization on growth and alkaloids of Datura innoxia Mill. plants. J Agr Rural Dev Trop. 104:151–165
• Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. J Plant Physiol 24(1):1
• Asha Rani NS, Prasad MP (2013) Studies on the organogenesis of Atropa Belladonna in in-vitro conditions. J Biotechnol Bioeng 4:457–464
• Bahmanzadegan JA, Sefidkon F, Sanboli A (2009) Determination of hyoscyamine and scopolamine in Four Hyoscyamus species from Iran. Iranian J Pharm Res 8(1):65–70
• Bennett M (1972) Nuclear DNA content and minimum generation time in herbaceous plants. Proc Roy Soc Lon Ser B Biol Sci 181(1063):109–135
• Bhattacharyya R, Bhattacharya S (2001) High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture. Indian J Exp Biol 39(11):1184–1187
• Chavadej S, Becker H (1984) Influence of Colchicine treatment on chromosome number and growth rate of tissue Culture of Valeriana wallichii DC. Plant Cell Tiss Organ Cult. 3:265–272
• Chen LL, Gao SL (2007) In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus. Sci Hort 112(3):339–344
• Cheng ZM, Korban SS (2011) In vitro ploidy manipulation in the genomics era. Plant Cell Tiss Organ Cult 104:281–282
• Dehghan E, Häkkinen ST, Oksman-Caldentey KM, Ahmadi FS (2012) Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tissue Organ Cult 110(1):35–44
• Deivis TH (1978) Flora of Turkey, vol 6. University Press, Edinburg, pp 454–455
• Dhawan OP, Lavania UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87:81–89
• Gantait S, Mandal N, Bhattacharyya S, Das PK (2011) Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv Sciella. Plant Cell Tissue Organ Cult 106(3):485–493
• Gao S, Zhu D, Cai Z, Xu D (1996) Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tissue Organ Cult 47(1):73–77
• Ghafarzadegan R, Khadiv PP, Khalighi Sigaroudi F, Pirali Hamedani M, Kadkhoda Z, Rezazadeh SA (2010) Optimization of extraction method of hyoscine from hyoscyamus niger L. J Med Plants 9(36):87–95
• Ghotbi Ravandi E, Rezanejad F, Zolala J, Dehghan E (2013) The effects of chromosome-doubling on selected morphological and phytochemical characteristics of Cichorium intybus L. J Hortic Sci Biotech 88(8):701–709
• Gopi C, Sekhar YN, Ponmurugan P (2006) In vitro multiplication of Ocimum gratissimum L. through direct regeneration. Afr J Biotechnol 5:9
• Gu X, Yang A, Meng H, Zhang J (2005) In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua. Plant Cell Rep 24(11):671–676
• Hashimoto T, Yamada Y (1994) Alkaloid biogenesis: molecular aspects. Annu Rev Plant Phys 45:257–285
• Heping H, Shanlin G, Lanlan C, Xiaoke J (2008) In vitro induction and identification of autotetraploids of Dioscorea zingiberensis. In Vitro Cell Dev-Pl 44(5):448–455
• Ionkova I (2010) Tropane alkaloids in Hyoscyamus reticulatus L. In: Awaad AS, Govil JN, Singh VK Drug plants I. pp 373–389
• Kaensaksiri T, Soontornchainaksaeng P, Soonthornchareonnon N, Prathanturarug S (2011) In vitro induction of polyploidy in Centella asiatica (L.) Urban. Plant Cell Tissue Organ Cult 107(2):187–194
• Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep 5(4):239–242
• Kang Y, Min J, Moon H, Karigar C, Prasad D, Lee C, Choi M (2004) Rapid in vitro adventitious shoot propagation of Scopolia parviflora through rhizome cultures for enhanced production of tropane alkaloids. Plant Cell Rep 23(3):128–133
• Kayani HA, Khan S, Naz S, Chaudhary MI (2013) Micropropagation of Agastache anisata using nodal segments as explants and cytotoxic activity of its methanolic extracts. Pak J Bot 45(6):2105–2109
• Koul S, Sambyal M, Kitchlu S, Bakshi S, Kaul M (2010) Development, micropropagation and characterization of colchiploid of Echinacea purpurea (L.) Moench. Indian J Biotech 9:221–224
• Kutchan TM (1995) Alkaloid biosynthesis [mdash] the basis for metabolic engineering of medicinal plants. Plant Cell 7(7):1059
• Lavania U (2005) Genomic and ploidy manipulation for enhanced production of phyto-pharmaceuticals. Acta Hortic 3(2):170–177
• Levin DA (1983) Polyploidy and novelty in flowering plants. Am Nat 122(1):1–25
• Lin X, Zhou Y, Zhang J, Lu X, Zhang F, Shen Q, Wu S, Chen Y, Wang T, Tang K (2011) Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway. Biotechnol Appl Bioc 58(1):50–57
• Liu Z, Gao S (2007) Micropropagation and induction of autotetraploid plants of Chrysanthemum cinerariifolium (Trev.) Vis. In vitro Cell Dev-Pl 43(5):404–408
• Liu S, Chen S, Chen Y, Guan Z, Yin D, Chen F (2011) In vitro induced tetraploid of Dendranthema nankingense (Nakai) Tzvel. shows an improved level of abiotic stress tolerance. Sci Hort 127(3):411–419
• Maharana SB, Mahato V, Behera M, Mishra RR (2012) In vitro regeneration from node and leaf explants of Jatropha curcas L. and evaluation of genetic fidelity through RAPD markers. Indian J Biotechnol 11:280–287
• Manske RRHF, Holmes HHL (1950) The alkaloids: chemistry and physiology V1. J Chem Physiol 1:1–525
• Mathura S, Fossey A, Beck SL (2006) Comparative study of chlorophyll content in diploid and tetraploid black wattle (Acacia mearnsii). Forestry 79(4):381–388
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
• Omidbaigi R (1997) Approaches to production and processing of medicinal plants, vol 2. Tarrahane Nashr Public Tehran 14:70–78
• Ovečka M, Bobák M, Šamaj J (1997) Development of shoot primordia in tissue culture of Papaver somniferum L. Biol Plant 39(4):499–506
• Philipov S, Berkov S (2002) GC-MS investigation of tropane alkaloids in Datura stramonium. Z Naturforsch C 57(5/6):559–561
• Pryor R, Frazier L (1968) Colchicine-induced tetraploid azaleas. Sci Hort 3(4):283–286
• Roy A, Leggett G, Koutoulis A (2001) In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Rep 20(6):489–495
• Shahriari-Ahmadi F, Dehghan E, Farsi M, Azizi M (2009) Tetraploid induction of Hyoscyamus muticus L. using colchicine treatment. Pak J Biol Sci 11(24):2653–2659
• Stanys V, Weckman A, Staniene G, Duchovskis P (2006) In vitro induction of polyploidy in Japanese quince (Chaenomeles japonica). Plant Cell Tissue Organ Cult 84(3):263–268
• Sundari M, Benniamin A, Manickam V (2010) Micropropagation and in vitro flowering in Solanum nigrum Linn. a medicinal plant. Int J Biotechnol 1(1):29–32
• Swanson CP (1957) Cytology and cytogenetics. Carl P, Swanson Urwin NA, Horsnell J, Moon T (2007) Generation and characterisation of colchicine-induced autotetraploid Lavandula angustifolia. Euphytica 156(1–2):257–266
• Viehmannová I, Cusimamani EF, Bechyne M, Vyvadilová M, Greplová M (2009) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius). Plant Cell Tissue Organ Cult 97(1):21–25
• Wendel J, Doyle J (2005) Polyploidy and evolution in plants. Plant diversity and evolution: genotypic and phenotypic variation in higher plants, p 97
• Wolfe KH (2001) Yesterday’s polyploids and the mystery of diploidization. Nat Rev Genet 2(5):333–341
• Zhang Z, Dai H, Xiao M, Liu X (2008) In vitro induction of tetraploids in Phlox subulata L. Euphytica 159(1–2):59–65
• Zhang Q, Luo F, Liu L, Guo F (2010) In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.). Plant Cell Tissue Organ Cult 101(1):41–47
• Zia M, Rehman R, Chaudhary MF (2007) Hormonal regulation for callogenesis and organogenesis of Artemisia absinthium L. Afr J Biotechnol 6:16

Identyfikatory

DOI

10.1007/s11738-015-1795-x