Expression of NK1 receptor at the protein and mRNA level in the porcine female reproductive system

• Autorzy: Bukowski R.
• Języki publikacji: EN

Abstrakty

EN

The presence and distribution of substance P (SP) receptor NK1 was studied in the ovary, the oviduct and the uterus (uterine horn and cervix) of the domestic pig using the methods of molecular biology (RT-PCR and immunoblot) and immunohistochemistry. The expression of NK1 receptor at mRNA level was confirmed with RT-PCR in all the studied parts of the porcine female reproductive system by the presence of 525 bp PCR product and at the protein level by the detection of 46 kDa protein band in immunoblot. Immunohistochemical staining revealed the cellular distribution of NK1 receptor protein. In the ovary NKI receptor was present in the wall of arterial blood vessels, as well as in ovarian follicles of different stages of development. In the tubular organs the NK1 receptor immunohistochemical stainings were observed in the wall of the arterial blood vessels, in the muscular membrane, as well as in the mucosal epithelium.The study confirmed the presence of NK1 receptor in the tissues of the porcine female reproductive tract which clearly points to the possibility that SP can influence porcine ovary, oviduct and uterus.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 3
• Opis fizyczny: p.469-477,fig.,ref.

Twórcy

autor

Bukowski R.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-957 Olsztyn, Poland

Bibliografia

• Angelova P, Davidoff M, Kanchev L, Baleva-Ivanova K (1991) Substance P: immunocytochemical localization and biological role in hamster gonads during ontogenesis. Funct Dev Morphol 1: 3-8.
• Assil IQ, Abou-Samra AB (2001) N-glycosylation of CRF receptor type 1 is important for its ligand-specific interaction. Am J Physiol Endocrinol Metab 281: E1015-E1021.
• Bodelsson G, Stjernquist M (1992) Smooth muscle dilatation in the human uterine artery induced by substance P, vasoactive intestinal polypeptide, calcitonin gene-related peptide and atrial natriuretic peptide: relation to endothelium- derived relaxing substances. Hum Reprod 7: 1185-1188.
• Brunsson I, Fahrenkrug J, Jodal M, Sjoqvist A, Lundgren O (1995) Substance P effects on blood flow, fluid transport and vasoactive intestinal polypeptide release in the feline small intestine. J Physiol 483 (Pt 3): 727-734.
• Burcher E, Buck SH, Lovenberg W, O’Donohue TL (1986) Characterization and autoradiographic localization of multiple tachykinin binding sites in gastrointestinal tract and bladder. J Pharmacol Exp Ther 236: 819-831.
• Burcher E, Zeng XP, Strigas J, Shang F, Millard RJ, Moore KH (2000) Autoradiographic localization of tachykinin and calcitonin gene-related peptide receptors in adult urinary bladder. J Urol 163: 331-337.
• Candenas ML, Magraner J, Armesto CP, Anselmi E, Nieto PM, Martin JD, Advenier C, Pinto FM (2001) Changes in the expression of tachykinin receptors in the rat uterus during the course of pregnancy. Biol Reprod 65: 538-543.
• Cao YQ, Mantyh PW, Carlson EJ, Gillespie AM, Epstein CJ, Basbaum AI (1998) Primary afferent tachykinins are required to experience moderate to intense pain. Nature 392: 390-394.
• Dangoor D, Giladi E, Fridkin M, Gozes I (2005) Neuropeptide receptor transcripts are expressed in the rat clitoris and oscillate during the estrus cycle in the rat vagina. Peptides 26: 2579-2584.
• Drews J (2000) Drug discovery: a historical perspective. Science 287: 1960-64.
• Elde R, Schalling M, Ceccatelli S, Nakanishi S, Hokfelt T (1990) Localization of neuropeptide receptor mRNA in rat brain: initial observations using probes for neurotensin and substance P receptors. Neurosci Lett 120: 134-138.
• Frieling T, Dobreva G, Weber E, Becker K, Rupprecht C, Neunlist M, Schemann M (1999) Different tachykinin receptors mediate chloride secretion in the distal colon through activation of submucosal neurones. Naunyn Schmiedebergs Arch Pharmacol 359: 71-79.
• Garcia-Fernandez MO, Solano RM, Carmena MJ, Busto R, Bodega G, Ruiz-Villaespesa A, Prieto JC, Sanchez-Chapado M (2003) Expression of functional PACAP/VIP receptors in human prostate cancer and healthy tissue. Peptides 24: 893-902.
• Goto T, Tanaka T (2002) Tachykinins and tachykinin receptors in bone. Microsc Res Tech 58: 91-97.
• Gram BR, Ottesen B (1982) Increased myometrial blood flow evoked by substance P. Pflugers Arch 395: 347-350.
• Hartung HP, Wolters K, Toyka KV (1986) Substance P: binding properties and studies on cellular responses in guinea pig macrophages. J Immunol 136: 3856-3863.
• Hokfelt T, Bartfai T, Bloom F (2003) Neuropeptides: opportunities for drug discovery. Lancet Neurol 2: 463-472.
• Hokfelt T, Broberger C, Xu ZQ, Sergeyev V, Ubink R, Diez M (2000) Neuropeptides--an overview. Neuropharmacology 39: 1337-1356.
• Hokfelt T, Lundberg JM, Schultzberg M, Johansson O, Ljungdahl A, Rehfeld J (1980) Coexistence of peptides and putative transmitters in neurons. Adv Biochem Psychopharmacol 22: 1-23.
• Ichikawa S, Sreedharan SP, Owen RL, Goetzl EJ (1995) Immunochemical localization of type I VIP receptor and NK-1-type substance P receptor in rat lung. Am J Physiol 268: L584-L588.
• Kaltreider HB, Ichikawa S, Byrd PK, Ingram DA, Kishiyama JL, Sreedharan SP, Warnock ML, Beck JM, Goetzl EJ (1997) Upregulation of neuropeptides and neuropeptide receptors in a murine model of immune inflammation in lung parenchyma. Am J Respir Cell Mol Biol 16: 133-144.
• Korman LY, Sayadi H, Bass B, Moody TW, Harmon JW (1989) Distribution of vasoactive intestinal polypeptide and substance P receptors in human colon and small intestine. Dig Dis Sci 34: 1100-1108.
• Lundberg JM, Brodin E, Hua X, Saria A (1984) Vascular permeability changes and smooth muscle contraction in relation to capsaicin-sensitive substance P afferents in the guinea-pig. Acta Physiol Scand 120: 217-227.
• Majewski M (1997) Afferent and efferent innervation of the porcine ovary - sources of origin and chemical coding. Acta Acad Agricult Tech Olst Vet 24.
• Mantyh PW (1991) Substance P and the inflammatory and immune response. Ann N Y Acad Sci 632: 263-271.
• Mantyh PW, Mantyh CR, Gates T, Vigna SR, Maggio JE (1988) Receptor binding sites for substance P and substance K in the canine gastrointestinal tract and their possible role in inflammatory bowel disease. Neuroscience 25: 817-837.
• Mechiche H, Koroglu A, Candenas L, Pinto FM, Birembaut P, Bardou M, Elaerts J, Devillier P (2003) Neurokinins induce relaxation of human pulmonary vessels through stimulation of endothelial NK1 receptors. J Cardiovasc Pharmacol 41: 343-355.
• Nimmo AJ, Whitaker EM, Carstairs JR, Morrison JF (1989) The autoradiographic localization of calcitonin gene-related peptide and substance P receptors in human fallopian tube. Q J Exp Physiol 74: 955-958.
• Ohtori S, Takahashi K, Chiba T, Yamagata M, Sameda H, Moriya H (2002) Substance P and calcitonin gene-related peptide immunoreactive sensory DRG neurons innervating the lumbar intervertebral discs in rats. Ann Anat 184: 235-240.
• Ottesen B, Gram BR, Fahrenkrug J (1983) Neuropeptides in the female genital tract: effect on vascular and non-vascular smooth muscle. Peptides 4: 387-392.
• Owman C, Stjernquist M (2003) Origin, distribution and functional aspects of aminergic and peptidergic nerves in the male and female reproductive tracts. In: Bjorklund A, Hokfelt T, Owman C (eds) The Peripheral Nervous System. Elsevier Science Publishers BV, Amsterdam, pp. 445-544.
• Patak E, Candenas ML, Pennefather JN, Ziccone S, Lilley A, Martin JD, Flores C, Mantecon AG, Story ME, Pinto FM (2003) Tachykinins and tachykinin receptors in human uterus. Br J Pharmacol 139: 523-532.
• Pintado CO, Pinto FM, Pennefather JN, Hidalgo A, Baamonde A, Sanchez T, Candenas ML (2003) A role for tachykinins in female mouse and rat reproductive function. Biol Reprod 69: 940-946.
• Pinter E, Szolcsanyi J (1995) Plasma extravasation in the skin and pelvic organs evoked by antidromic stimulation of the lumbosacral dorsal roots of the rat. Neuroscience 68: 603-614.
• Pinto FM, Armesto CP, Magraner J, Trujillo M, Martin JD, Candenas ML (1999) Tachykinin receptor and neutral endopeptidase gene expression in the rat uterus: characterization and regulation in response to ovarian steroid treatment. Endocrinology 140: 2526-2532.
• Pisarchik A, Slominski A (2002) Corticotropin releasing factor receptor type 1: molecular cloning and investigation of alternative splicing in the hamster skin. J Invest Dermatol 118: 1065-1072.
• Rasley A, Bost KL, Olson JK, Miller SD, Marriott I (2002) Expression of functional NK-1 receptors in murine microglia. Glia 37: 258-267.
• Rothstein RD, Johnson E, Ouyang A (1991) Distribution and density of substance P receptors in the feline gastrointestinal tract using autoradiography. Gastroenterology 100: 1576-1581.
• Seybold VS, McCarson KE, Mermelstein PG, Groth RD, Abrahams LG (2003) Calcitonin gene-related peptide regulates expression of neurokinin1 receptors by rat spinal neurons. J Neurosci 23: 1816-1824.
• Togari A, Arai M, Mizutani S, Mizutani S, Koshihara Y, Nagatsu T (1997) Expression of mRNAs for neuropeptide receptors and beta-adrenergic receptors in human osteoblasts and human osteogenic sarcoma cells. Neurosci Lett 233: 125-128.
• Villablanca AC, Hanley MR (1997) 17beta-estradiol stimulates substance P receptor gene expression. Mol Cell Endocrinol 135: 109-117.
• Wasowicz K (2003) The effect of total or partial uterus extirpation on the uterus-projecting neurons in the porcine inferior mesenteric ganglion (IMG). A. Changes in the expression of transmitter-synthesizing enzymes - tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH) and choline acetyltransferase (ChAT). Pol J Vet Sci 6: 131-145.

Identyfikatory

DOI

10.2478/pjvs-2014-0068