DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

• Autorzy: Pietron W.J. , Golebiowska A. , Paprocka I. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The aim of this paper was to present the results of comparative evaluation of the usefulness of PCR method for the detection and identification of bovine DNA in feeds. In the validation study, the limit of detection for PCR was determined as 0.05% for bovine meat and bone meal (MBM). Among 132 feed samples, bovine DNA was detected in eight (6.06%) samples. In the next stage of the study, sediment and flotate from the investigated samples were examined with PCR. Out of 132 sediment and flotate samples, bovine DNA was detected in eight (6.06%) and nine (6.82%) samples, respectively. On the basis of the results obtained with the use of the PCR, it is possible to state that the molecular biology methods can, at present, be used as supplementary tools for detection and identification of bovine MBM.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 4
• Opis fizyczny: p.553-557,fig.,ref.

Twórcy

autor

Pietron W.J.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Golebiowska A.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Paprocka I.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Arslan A., Irfan Ilhak O., Calicioglu M.: Effect of method of cooking on identification of heat processed beef using polymerase chain reaction (PCR) technique. Meat Sci 2006, 7, 326-330.
• 2. Boom R., Sol C., Beld M., Weel J., Goudsmith J., Wertheim-van Dillen P.: Improved silicaguanidiniumthiocyanate DNA isolation procedure based on binding of bovine alpha casein to silica particles. J Clin Microbiol 1999, 37, 615-619.
• 3. Bottero M.T., Civera T., Nucera D., Turi R.M.: Design of universal primers for the detection of animal tissues in feedstuff. Vet Res Commun 2003, 27, Suppl. 1, 667-669.
• 4. Bottero M.T., Dalmasso A., Nucera D., Turi R.M., Rosati S., Squadrone S., Goria M., Civera T.: Development of a PCR assay for the detection of animal tissues in ruminant feeds. J Food Prot 2003, 66, 2307-2312.
• 5. Buntjer J.B., Lenstra J.A.: Mammalian species identification by interspersed repeat PCR fingerprinting. J Ind Microbiol Biotech 1998, 21, 121-127.
• 6. Colgan S., O'Brien L., Maher M., Shilton N., McDonnel K., Ward S.: Development of a DNA based assay for species identification in meat and bone meal. Food Res Int 2001, 34, 409-414.
• 7. Commision Decision of 29 December 2000 concerning control measures required for the implementation of Council Decision 2000/766/EC concerning certain protection measures with regard to transmissible spongiform encephalopathies and the feeding of animal protein (O.J.L 2/32, 05.01.2001).
• 8. Commission Regulation (EC) No 152/2009 of January 2009 laying down the methods of sampling and analysis for the official control of feed (O.J.L 54/1, 26.2.2009).
• 9. Commission Regulation (EC) No. 1234/2003 of 10 July 2003 amending Annexes I, IV and Xl to Regulation (EC) No. 999/2001 of the European Parliament and of the Council and Regulation (EC) No. 1326/2001 as regards transmissible spongiform encephalopathies and animal feeding (O.J.L 173, 11.07.2003).
• 10. Commission Regulation (EC) No. 1292/2005 of 5 August 2005 amending Annex IV to Regulation (EC) No. 999/2001 of the European Parliament and of the Council as regards animal nutrition (O.J.I. 205/3, 6.8.2005).
• 11. Communication from the Commission to the European Parliament and the Council. THE TSE Roadmap 2. A strategy paper on Transmissible Spongiform Encephalopathies for 2010-2015. Brussels, 16.07.2010. COM(2010) 384 final. SEK(2010)899.
• 12. Dalmasso A., Fontanella E., Piatti P., Civera T., Rosati S., Bottero M. T.: A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes 2004, 18, 81-87.
• 13. Engling F.P., Jörgensen J.S., Paradies-Severin I., Hahn H.: Evidence of animal meal in feeds. Kraftfutter/Feed Magazine 2000, 1, 14-17.
• 14. Frezza D., Giambra V., Chegdani F., Fontana C., Maccabiani G., Losio N., Faggionato E., Chiappini B., Vaccari G., von Holst C., Lanni L., Saccares S., Ajmone-Marsan P.: Standard and light-cycler PCR methods for animal DNA species detection in animal feedstuffs. Innovative Food Sci Emerg Technol 2008, 9, 18-23.
• 15. Frick Ct., Roetschi A., Hauswirth H.: Mikroskopische Untersuchung von Futtermitteln. Agrarforschung 2002, 9, 497-504.
• 16. Frick G., Roetschi A., Hauswirth H.: Futtermittel: Prüfung der Zusammensetzung. Agrarforschung 2003, 10, I-VIII.
• 17. Fumiere O., Dubois M., Baeten V,. von Holst C., Berben G.: Effective PCR detection of animal species in highly processed animal byproducts and compound feeds. Anal Bioanal Chem 2006, 385, 1045-1054.
• 18. Fumiere O., Veys P., Boix A., von Holst C., Baeten V., Berben G.: Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs. Biotechnol Agron Soc Environ 2009, 13, 59-70.
• 19. Gizzi G., von Holst Ch.: Determination of processed animal proteins, including meat and bone meal, in animal feed. J AOAC Int 2004, 87, 1334-1341.
• 20. Gizzi G., von Holst C., Baeten V., Berben G.: Raamsdonk L.W.D.: Intercomparison study for the determination of processed animal proteins including meat and bone meal in animal feed. Final report of 16 May 2003.
• 21. Gizzi G., Raamsdonk L.W.D., Baeten V., Murray I., Berben G., Brambilla G., von Holst C.: An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy. Rev Sci Tech Off Int Epiz 2003, 22, 311 -331.
• 22. Gouli Z., Mingguang Z., Zhijiang Z., Hongsheng O., Qiang I.: Establishment and application of polymerase chain reaction for the identification of beef. Meat Sci 1996, 51, 233- 236.
• 23. Hahn H. Animal meal: production and determination in feedstuffs and the origin of bovine spongiform encephalopathy. Naturwissenschaften 1996, 86, 62-70.
• 24. Heidenreich E.: Zero tolerance or minimised residual risk? Kraftfutter/Feed Magazine 2001, 10, 367-371.
• 25. Hormish D.E.: Traceability of processed animal proteins with varying texture in feed: determination with microscopic and polymerase chain reaction methods. Biotechnol Agron Soc Environ 2004, 8, 257-266.
• 26. Kingombe C.I.B., Lüthi E., Schlosser H., Howald D., Kuhn M., Jemmi T.: A PCR based test for species-specific determination of heat treatment conditions of animal meals as an effective prophylactic method for bovine spongiform encephalopathy. Meat Sci 2001, 57, 35-41.
• 27. Krčmàř P., Renčovà E.: Identification of bovine-specific DNA in feedstuffs. J Food Prot 2001, 64, 117-119.
• 28. Kwiatek K., Weiner A., Przeniosło M.: Two-year study on prevalence of processed animal proteins in feedingstuffs. Bull Vet Inst Pulawy 2007, 51, 603-607.
• 29. Lahiff S., Glennon M., O'Brien L., Lyng J., Smith T., Maher M., Shilton N.: Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM). Mol Cell Probes 2001, 15, 27-35.
• 30. Martin I., Garcia T., Fajardo V., López Calleja I., Hernández P.E., Gonazález I., Martín R.: Species specific PCR for the identification of ruminant species in feedstuffs. Meat Sci 2007, 75, 120-127.
• 31. Martin I., Garcia T., Fajardo V., Rojas M., Hernández P.E., Gonazález I., Martín R.: technical note: Detection of cat, dog and rat or mouse in food animal feed using species-specific polymerase chain reaction. J Anim Sci 2007, 85, 2734-2739.
• 32. Meyer R., Candrian U., Lüthy J.: Detection of pork in heated meat products by the polymerase chain reaction. J AOAC Int 1994, 77, 617-622.
• 33. Pinotti L., Campagnoli A., Tongon G., Cheli F., Dell'Orto V., Savoini G.: Microscopic method in processed animal proteins identification in feed: applications of image analysis. Biotechnol Agron Soc Environ 2004, 8, 249-251.
• 34. Prado M., Casqueiro J., Iglesias Y., Cepeda A., Barros-Veláquez J.: Application of polymerase chain reaction (PCR) method as a complementary tool to microscopic analysis for the detection of bones and other animal tissues in home-made animal meals. J Sci Food Agrie 2004, 84, 505-512.
• 35. Raamsdonk L.W.D., Vancustem J., Zegers J., Frick G., Jorgenson J.S., Pinckaers V., Bosch J., Paradies Severin I.: The microscopic detection of animal proteins in feeds. Biotechnol Agron Soc Environ 2004, 8, 241-247.
• 36. Regulation (EC) No 1069/2009 of the European Parliament and of the Council of October 2009 laying down health rules as regards animal by-products and derived products not intended for human consumption and repealing Regulation (EC) No 1774/2002 (animal by-products regulation) (O.J.L 300/1, 14.11.2009).
• 37. Regulation (EC) No. 1774/2002 of the European Parliament and of the Council of 3 October 2002 laying down health rules concerning animal by-products not intended for human consumption (O.J.L. 273. 10.10.2002).
• 38. Regulation (EC) No. 999/2001 of the European Parliament of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies (O.J.L. 147.31.05.2001).
• 39. Rodriguez M.A., García T., Gonzáles I., Asensio L., Hernández P.E., Martín R.J.: PCR identifiaction of beef, sheep, goat and pork in raw and heat treated meat mixtures. J Food Prot 2004, 67, 172-177.
• 40. Saiki R.K., Gelfand D.H., Stoffel S.: Primer directed enzymatic amplification of DNA with a termostable DNA polymerase. Science 1988, 239, 487-491.
• 41. Tartaglia M., Saulle E., Pestalozza S., Morelli L., Antonucci G., Battaglia P.A.: Detection of bovine mitochondrial DNA in ruminant feeds: a molecular test for the presence of bovine-derived materials. J Food Prot 1998, 5, 513-518.
• 42. Unseld M., Beyermann B., Brant P., Hiesel R.: Identification of species origin of highly processed meat products by mitochondrial DNA sequences. PCR Meth Appl 1995, 4, 241 -243.
• 43. von Holst C., Baeten V., Berben G., Brambilla G.: Overview of methods for the detection of species specific proteins in feed intended for farmed animals. European Commission. IRMM. September 2004.
• 44. Wolf H., Gaede W., Wolf C., Zellermann S., Höber S.: Nachweis von tierischen Bestandteilen im Mischfutter. Vergleichende Untersuchungen. Fleischwirtschaft 2001, 5, 197-201.

Identyfikatory

DOI

10.2478/bvip-2013-0095