The effect of low temperature and GA3 treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro

• Autorzy: Petric M. , Jevremovic S. , Trifunovic M. , Tadic V. , Milosevic S. , Dragicevic M. , Subotic A.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of low temperature and gibberellic acid (GA3) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4–8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 °C) or at low temperatures (4 and 15 °C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 °C. To investigate the influence of GA3 on dormancy, bulbs were treated for 24 h with GA3 solutions with 1, 2 and 3 mg l−1 concentrations. During the period of growth of bulbs at 4 °C, regeneration of bulbs was very weak, while at 15 °C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 11
• Opis fizyczny: p.3223-3236,fig.,ref.

Twórcy

autor

Petric M.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Jevremovic S.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Trifunovic M.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Tadic V.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Milosevic S.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Dragicevic M.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

autor

Subotic A.

• Department for Plant Physiology, Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia

Bibliografia

• Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126
• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
• Aung LH, De Hertogh AA (1979) Temperature regulation of growth and endogenous abscisic acid-like content of Tulipa gesneriana L. Plant Physiol 63:1111–1116
• Baek KH, Skinner DZ (2003) Alternation of antioxidant enzyme gene expression during cold acclimation of near isogenic wheat lines. Plant Sci 165:1221–1227
• Banasik I, Rudnicki RM, Saniewski M (1980) The physiology of hyacinth bulbs (Hyacinthus orientalis L.). XIII. The distribution of amylase and acid phosphatase activities and starch grain in hyacinth bulbs. Acta Physiol Plant 2:145–156
• Beukema HP, van der Zaag DE (1990) Introduction to potato production. Wageningen, Pudoc
• Bewley JD (1997) Seed gemination and dormancy. Plant Cell 9:1055–1066
• Beyer WF, Fridowich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161:559–566
• Bowler C, Van Camp W, Van Montagu M, Inze´ D (1994) Superoxide dismutases in plants. Crit Rev Plant Sci 13:199–218
• Bradford MM (1976) A rapid and sensitive method for the quantitation pf microgram quantities of protein utilizing the principle of protein binding. Anal Biochem 72:248–254
• Cakmak I, Strbac D, Marschner H (1993) Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J Exp Bot 44:127–132
• Carlberg I, Mannervik B (1985) Glutathione reductase. Method Enzymol 113:484–490
• De Hertogh AA, Le Nard M (1993) Physiological and biochemical aspects of flower bulbs. In: De Hertogh AA, Le Nard M (eds) The physiology of flower bulbs. Elsevier, Amsterdam, pp 53–69
• De Klerk GJ (2009) A cold treatment promotes both sprouting and sink strength of lily bulblets. Propag Ornam Plants 9:102–106
• Fadzillah NM, Gill V, Finch RP, Burdon RH (1996) Chilling, oxidative stress and antioxidant responses in shoot cultures of rice. Planta 199:552–556
• Fontaine O, Huault C, Pavis N, Billard JP (1994) Dormancy breakage of Hordeum vulgare seeds: effects of hydrogen peroxide and stratification on glutathione and glutathione reductase activity. Plant Physiol Biochem 32:677–683
• Gorin N, Heidema FT (1985) Starch content of freeze-dried anthers and alpha-amylase activity of their extracts as criteria that dried-stored bulbs (Tulipa gesneriana L.) ‘Apeldoorn’ have been exposed to 5 º C. Sci Hort 26:183–189
• Hendricks SB, Taylorson RB (1975) Breaking of seed dormancy by catalase inhibition. Proc Natl Acad Sci USA 72:306–309
• Higuchi H, Sisa M (1967) Serological analysis on the change of protein in scaly leaf bulb caused by low temperature treatment. J Jpn Soc Hort Sci 36:55–60
• Hobson GE, Davies JN (1977) Mitochondrial activity and carbohydrate levels in tulip bulbs in relation to cold treatment. J Exp Bot 28:559–568
• Hodges DM, Andrews CJ, Johnson DA, Hamilton RI (1997a) Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines. J Exp Bot 48:1105–1113
• Hodges DM, Andrews CJ, Johnson DA, Hamilton RI (1997b) Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids. Crop Sci 37:857–863
• Hulscher M, Krijksheld HT, Van Der Linde PCG (1992) Propagation of shoots and bulbs growth of tulip in vitro. Acta Hort 325:441–446
• Inze´ D, Montagu M (1995) Oxidative stress in plants. Curr Opin Biotech 6:153–158
• Jahnke LS, Hull MR, Long SP (1991) Chilling stress and oxygen metabolizing enzymes in Zea mays and Zea diploperennis. Plant Cell Environ 14:97–104
• Jevremović S, Petrić M, Živković S, Trifunović M, Subotić S (2010) Superoxide dismutase activity and isoenzyme profiles in bulbs of snake’s head fritillary in response to cold treatment. Arch Biol Sci 62:553–558
• Kamenetsky R, Zemah H, Ranwala AP, Vergeldt F, Ranwala NK, Miller WB, Van As H, Bendel P (2003) Water status and carbohydrate pools in tulip bulbs during dormancy release. New Phytol 158:109–118
• Kaminaka H, Morita S, Tokumoto M, Masumura T, Tanaka K (1999) Differential gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radic Res 31:219–225
• Kerdnaimongkol K, Woodson WR (1999) Inhibition of catalase by antisense RNA increases susceptibility to oxidative stress and chilling injury in transgenic tomato plants. J Am Soc Hort Sci 124:330–336
• Kim KS (1991) The effect of growth regulators, temperature and sucrose on the dormancy in Lilium speciosum bulblets cultured in vitro. Korean J Plant Tissue Cult 18:103–111
• Kukavica B, Veljović-Jovanović S (2004) Senescence-related changes in antioxidant status of ginkgo and birch leaves during autumn yellowing. Physiol Plant 122:321–327
• Kukulczanka K, Kromer K, Czastka B (1989) Propagation of Fritillaria meleagris L. through tissue culture. Acta Hort 251:147–153
• Langens-Gerrits MM, Miller WB, Lilien-Kipnis H, Kollöffel C, Croes T, De Klerk GJ (1997) Bulb growth in lily regenerated in vitro. Acta Hort 430:267–273
• Langens-Gerrits MM, Nashimoto S, Croes AF, De Klerk GJ (2001) Development of dormancy in different lily genotypes regenerated in vitro. Plant Growth Regul 34:215–222
• Langens-Gerrits MM, Miller WB, Croes AF, Klerk GJ (2003a) Effect of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Plant Growth Regul 40:267–275
• Langens-Gerrits MM, Klerk GJ, Croes AF (2003b) Phase change in lily bulblets regenerated in vitro. Physiol Plant 119:590–597
• Le Nard M (1983) Physiology and storage of bulbs: concept and nature of dormancy in bulbs. In: Liberman M (ed) Post harvest physiology and crop preservation, NATO Advanced Study Institute, series a: life science. Plenum Press, New York, pp 191–230
• Le Nard M, De Hertogh AA (1993) Bulb growth and development and flowering. In: De Hertogh A, Le Nard M (eds) Physiology of flower bulbs. Elsevier, Amsterdam, pp 29–43
• Ledesma A, Reate MI, Racea R, Burba JL (1980) Effect of low temperature and preplanting storage time on garlic clonal type Rosado Paraguya growth. Phyton 39:37–48
• Lee DH, Lee CB (2000) Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci 159:75–85
• Lee HS, Kim KY, You SH, Kwon SY, Kwak SS (1999) Molecular characterization and expression of cDNA encoding copper/zinc superoxide dismutase from cultured cells of cassava (Manihot esculenta Crantz). Mol Gen Genet 262:807–814
• Li CJ, Qin ZD (1987) The relationship between physiological and biochemical changes and releasing dormancy of Fritillaria pallidiflora Schrenk. during low temperature treatment. Acta Bot Boreali-Occidential Sin 7:23–28
• Lukatkin AS (2002) Contribution of oxidative stress to the development of cold-induced damage to leaves of chilling-sensitive plants: 2. The activity of antioxidant enzymes during plant chilling. Russ J Plant Physiol 49:782–788
• McKersie BD, Bowley SR, Jones KS (1999) Winter survival of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol 119:839–847
• Mizuno M, Kamei M, Tsuchida H (1998) Ascorbate peroxidase and catalase cooperate for protection against hydrogen peroxide generated in potato tubers during low-temperature storage. Biochem Mol Biol Int 44:717–726
• Moriconi DN, Conci VC, Nome SF (1990) Rapid multiplication of garlic (Allium sativum L.) in vitro. Phyton 51:145–151
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco culture. Physiol Plant 15:473–497
• Niimi Y, Endo Y, Arisaka E (1988) Effects of chilling and GA3 treatments on breaking dormancy in Lilium rubellum Baker bulblets cultured in vitro. J Jpn Soc Hort Sci 57:250–257
• Nir G, Shulman Y, Fanberstein L, Lavee S (1986) Changes in the activity of catalase (EC 1.11.1.6) in relation to the dormancy of grapevine (Vitis vinifera L.) buds. Plant Physiol 81:1140–1142
• Nowak J, Saniewsky M, Rudnicki RM (1974) Studies on the physiology of hyacinth bulb (Hyacinthus orientalis L.). Sugar content and metabolic activities in bulbs exposed to low temperatures. J Hort Sci 49:383–390
• Okagami N (2003) Dormancy in bulbils of several herbaceous plants: effects of photoperiod, light, temperature, oxygen and gibberellic acid. Trends Plant Sci 8:534–540
• Okagami N, Tanno N (1991) Dormancy in Dioscorea: comparation of dormant characters in bulbils of a northern species (D. opposita) and southern species (D. bulbifera var. vera). J Plant Physiol 138:559–565
• Okubo H, Iwaya-Inoue M, Motooka K, Ishida N, Kano H, Koizumi M (1997) Monitoring the cold requirements in tulip bulbs by 1HNMR imaging. Acta Hort 430:411–415
• Paek KY (1996) Several factors affecting bulblet regeneration from the culture of scale segment and node-bud in fritillary as medicinal bulbous plant. Acta Hort 440:498–503
• Paek KY, Murthy HN (2002) High frequency of bulblet regeneration from bulb scale section of Fritillaria thunbergii. Plant Cell Tissue Organ 68:247–252
• Petrić M, Subotić A, Jevremović S, Trifunović M (2011) Somatic embryogenesis and bulblet regeneration in snakehaed fritillary (Fritillaria meleagris L.). Afr J Biotechnol 10:16181–16188
• Pritchard SL, Charlton WL, Baker A, Graham IA (2002) Germination and storage reserve mobilization are regulated independently in Arabidopsis. Plant J 31:639–647
• Puntarulo S, Sanchez RA, Boveris A (1988) Hydrogen peroxide metabolism in soybean embryonic axes at the onset of germination. Plant Physiol 86:626–630
• Rahman MH, Haque MS, Karim MA, Ahmed M (2006) Effects of gibberellic acid (GA3) on breaking dormancy in garlic (Allium sativum L.). Int J Agric Sci 8:63–65
• Rakhimbaev IR, Syrtanova GA, Solomina VF (1978) Effect of cold treatment on the level of biological activity of endogenous growth regulation of tulip bulbs. Fiziol Rast Mosk 25:249–253
• Rebers M, Vermeer E, Knegt E, Shelton CJ, Van der Plas LHW (1995) Gibberellin levels and cold-induced floral stalk elongation in tulip. Physiol Plant 94:687–691
• Rohde A, Bhallerao P (2007) Plant dormancy in the perennial context. Trends Plant Sci 12:217–223
• Rojas-Beltran JA, Dejaeghere F, Abd Alla Kotb M, Du Jardin P (2000) Expression and activity of antioxidant enzymes during potato tuber dormancy. Potato Res 43:383–393
• Seppänen MM, Fagerstedt K (2000) The role of superoxide dismutase activity in response to cold acclimation in potato. Physiol Plant 108:279–285
• Seppänen MM, Majaharju M, Somersalo S, Pehu E (1998) Freezing tolerance, cold acclimation and oxidative stress in potato. Paraquat tolerance is related to acclimation process but is a poor indicator of freezing tolerance. Physiol Plant 102:454–460
• Shen WY, Nada K, Tachibana S (1999) Effect of cold treatment on enzymatic and non-enzymatic antioxidant activities in leaves of chilling tolerant and chilling sensitive cucumber (Cucumis sativus L.) cultivars. J Jpn Soc Hort Sci 68:967–973
• Shin KS, Chakrabarty D, Paek KY (2002) Sprouting rate, change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro. Sci Hort 96:195–204
• Slabbert MM, Niederwieser JG (1999) In vitro bulblet production of Lachenalia. Plant Cell Rep 18:620–624
• Specht CE, Keller ERJ (1997) Temperature requirements for seed germination in species of the genus Allium L. Genet Resour Crop Evol 44:509–517
• Steffen KL, Palta JP (1987) Photosynthesis as a key process in plant response to low temperature. In: Li PH (ed) Alteration during low temperature acclimation and impairment during incipient freeze-thaw injury, plant cold hardiness, plant biology, vol 5. Alan R. Liss, Inc., New York, pp 67–99
• Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Ann Rev Plant Physiol 35:543–584
• Subotić A, Trifunović M, Jevremović S, Petrić M (2010) Morphohistological study of direct somatic embryogenesis in endangered species Fritillaria meleagris. Biol Plant 54:592–596
• Sun CS, Wang DY (1991) Fritillaria spp. (Fritillary): in vitro culture and the regeneration of plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry: medicinal and aromatic plants III, vol 15. Springer, Germany, pp 258–269
• Tsang EWT, Bowler C, Herouart D, Van Camp W, Villarroel R, Genetello C, Van Montagu M, Inze´ D (1991) Differential regulation of superoxide dismutases in plants exposed to environmental stress. Plant Cell 3:783–792
• Vegis A (1964) Dormancy in higher plants. Annu Rev Plant Physiol 15:185–224
• Vyas D, Kumar S (2005) Tea (Camellia sinensis (L.) O. Kuntze) clone with lower period of winter dormancy exhibits lesser cellular damage in response to low temperature. Plant Physiol Biochem 43:383–388
• Walck JL, Cofer MS, Hidayati SN (2010) Understanding the germination of bulbils from an ecological perspective: a case study on Chinese yam (Dioscorea polystachya). Ann Bot 106:945–955
• Walker MA, McKersie BD (1993) Role of ascorbate–glutathione antioxidant system in chilling resistance of tomato. J Plant Physiol 141:234–239
• Yamazaki H, Ishida N, Katsura N, Kano H, Nishijima T, Koshioka M (1995) Changes in carbohydrate composition and water status during bulb development of Allium wakegi Araki. Bull Natl Res Inst Veg Ornam Plant Tea A 10:1–11
• Yamazaki H, Nishijima T, Koshioka M, Miura H (2002) Gibberellins do not act against abscisic acid in the regulation of bulb dormancy of Allium wakegi Araki. Plant Growth Regul 36:223–229
• Zemah H, Bendel P, Rabinowitch H, Kamenetsky R (1999) Visualization of morphological structure and water status during storage of Allium aflatunense bulbs by NMR imaging. Plant Sci 147:65–73
• Zhu SY, Hu ZH, Yu WQ (1980) Study on the annual periodicity of growth and development of Fritillaria pallidiflora Schrenk. J Integr Plant Biol 22:22–26
• Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Deberhh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer Academic Publishers, Dordrecht, pp 45–70

Uwagi

rekord w opracowaniu