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2011 | 33 | 4 |

Tytuł artykułu

Effect of alar and CCC on induction and germination of bulbils in Curculigo orchioides grown in shake flask cultures

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Bulbil formation in Curculigo orchioides is followed by asynchronous germination. The effect of alar and CCC incorporated in Murashige and Skoog (MS) medium has been studied on bulbil induction from leaf explants and subsequent germination of bulbils. MS medium contained 1 mg/l BA and 0.1 mg/l morphactin for bulbil induction while germination medium contained 1 mg/l gibberrelic acid and both the media contained alar or CCC (0.5–5.0 mg/l). Growth retardants markedly reduces the bulbil formation, yield and fresh weight of bulbils. Incorporation of retardants resulted in 60% germination inhibition, thereby prolonging the storage conditions.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

33

Numer

4

Opis fizyczny

p.1543-1545,fig.,ref.

Twórcy

autor
  • Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur, 313001, India
autor
  • Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur, 313001, India
  • Laboratory of Bio-Molecular Technology, Department of Botany, M.L. Sukhadia University, Udaipur, 313001, India

Bibliografia

  • Arora J, Roat C, Goyal S, Ramawat KG (2009) High stilbenes accumulation in root cultures of Cayratia trifolia (L.) Domin grown in shake flasks. Acta Physiol Plant 31:1307–1312
  • Dragicevic I, Konjevic R, Vinterhalter B, Vinterhalter D, Neskovic M (2008) The effects of IAA and tetcyclacis on tuberization in potato (Solanum tuberosum L.) shoot cultures invitro. Plant Growth Regul 54:189–193
  • El-Sheibany OM, El-Malki NA, Barras-Ali A (2007) Effect of application of growth retardant ALAR on some foliage characters of local cultivar of chrysanthemum. J Sci Appl 1:15–20
  • Francis SV, Senapati SK, Rout GR (2007) Rapid clonal propagation of Curculigo orchiodes Gaertn., an endangered medicinal plant. In vitro Cell Dev Biol 43:140–143
  • Gupta R, Chadha KL (1995) Medicinal and aromatic plant research in India. In: Chadha KL, Gupta R (eds) Advances in horticulture—medicinal and aromatic plants. Malhotra Pub. House, New Delhi, pp 429–451
  • Kumar S, Kashyap M, Sharma DR (2005) In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance. Biol Plant 49:629–632
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
  • Nagaraju V, Bhowmik G, Parthasarathy VA (2002) Effect of paclobutrazol and sucrose on in vitro cormel formation in Gladiolus. Act Bot Croatia 61:27–33
  • Nagesh KS, Shanthamma C, Bhagyalakshmi N (2009) Role of polarity in de novo shoot bud initiation from stem disc explants of Curculigo orchioides Gaertn. and its encapsulation and storability. Acta Physiol Plant 31:699–704
  • Nema RK, Dass S, Mathur M, Ramawat KG (2008) Morphactin and cytokinin promote high frequency bulbil formation from leaf explants of Curculigo orchioides grown in shake flasks cultures. Indian J Biotech 7:520–525
  • Rademacher W (2000) Growth Retardants: Effects on gibberellin biosynthesis and other metabolic pathways. Annu Rev Plant Physiol Plant Mol Biol 51:501–531
  • Rayirath UP, Lada RR, Caldwell CD, Asiebu SK, Sibley KJ, Adams AD (2009) CCC and prohexadione-Ca enhance rhizome growth and lateral bud production in Rhubarb (Rheum rhabarbarum L.). J Plant Growth Regul 28:137–146
  • Suri SS, Arora DK, Sharma R, Ramawat KG (1998) Rapid micropropagation through direct somatic embryogenesis and bulbil formation from leaf explants in Curculigo orchioides. Indian J Exp Biol 36:1130–1135
  • Suri SS, Jain S, Ramawat KG (1999) Plant regeneration and bulbils formation in vitro from leaf and stem explants of Curculigo orchioides: an endangered medicinal plant. Sci Hort 79:127–134
  • Suri SS, Arora DK, Ramawat KG (2000) A method for large-scale multiplication of Curculigo orcheiodes through bulbil formation from leaf explant in shake flask cultures. Indian J Exp Biol 38:145–148
  • Suthar S, Ramawat KG (2010) Growth retardants stimulate guggulsterone production in presence of fungal elicitor in fed batch cultures of Commiphora wightii. Plant Biotech Rep 4:9–13
  • Tandon M, Shukla YN (1995) Phytoconstituents of Asparagus adscendens, Chlorophytum arundinaceum and Curculigo orchioides: a review. Curr Res Med Aromat Plants 17:42–50
  • Wang H, Xiao L (2009) Effects of chlorocholine chloride on phytohormones and photosynthetic characteristics in potato (Solanum tuberosum L.). J Plant Growth Regul 28:21–27

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Bibliografia

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