Overexpression of suadea salsa S-adenosylmethionine synthetase gene promotes salt tolerance in transgenic tobacco

• Autorzy: Qi Y.-C. , Wang F.-F. , Zhang H. , Liu W.-Q.
• Języki publikacji: PL

Abstrakty

EN

The suadea salsa full-length S-adenosylmethionine synthetase (SsSAMS2) was introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens-mediated transformation. The gene transformation and expression in tobacco were confirmed by PCR, RT-PCR and Northern blotting analysis. Several transgenic lines (ST lines) overexpressing SsSAMS2 gene under the control of cauliflower mosaic virus 35S promoter showed more seeds number and weight, and accumulated higher free total polyamines (PAs) than wild-type plants (WT lines) and transformants with blank vector (BT lines). Salt stress-induced damage was attenuated in these transgenic plants, in the symptom of maintaining higher photosynthetic rate and biomass. These results that the transgenic plants overexpressing suadea salsa SAMS2 are more tolerant to salt stress than wild-type plants suggest that PAs may play an important role in contributing salt tolerance to plants.

Słowa kluczowe

EN

overexpression; S-adenosylmethionine synthetase; gene promoter; salt tolerance; transgenic plant; tobacco; Nicotiana tabacum; suaeda salsa; seepweed zob.suaeda salsa; Suaeda; Agrobacterium tumefaciens; salt stress; polyamine

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 2
• Opis fizyczny: p.263-269,fig.,ref.

Twórcy

autor

Qi Y.-C.

• College of Tobacco Science, Henan Agricultural University, 450002 Zhengzhou, China
• College of Life Science, Henan Agricultural University, 95 Wenhua Road, 450002 Zhengzhou, China

autor

Wang F.-F.

• College of Life Science, Henan Agricultural University, 95 Wenhua Road, 450002 Zhengzhou, China

autor

Zhang H.

• Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, 250014 Jinan, China

autor

Liu W.-Q.

• College of Life Science, Henan Agricultural University, 95 Wenhua Road, 450002 Zhengzhou, China

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