Fasciola hepatica – the pilot study of in vitro assessing immune response against native and recombinant antigens of the fluke

• Autorzy: Baska P. , Zawistowska-Deniziak A. , Zdziarska A. M. , Wasyl K. , Wisniewski M. , Cywinska A. , Klockiewicz M. , Januszkiewicz K. , Wedrychowicz H.
• Języki publikacji: EN

Abstrakty

EN

Fasciola hepatica is a liver fluke that infects 2.4 million of people and causes great economical loss in animal production. To date a 100% effective vaccine has not been developed and the disease is controlled by drug therapy. Great efforts are put into development of effective vaccine against parasite what is difficult since Fasciola spp. (like other helmints) during evolutionary process has developed sophisticated and efficient methods to evade immune response. During preliminary experiments it is convenient to use cell lines which are relatively cheap and allow for reproducible comparison of results between laboratories. We stimulated BOMA (bovine monocyte/macrophage cell line) and BOMAC (bovine macrophage cell line) with native or recombinant antigens of Fasciola hepatica and assessed IFN-γ, IL-4 and TNF-α level upon stimulation. We observed diminished secretion of proinflammatory TNF-α in LPS activated BOMA cells stimulated with Excretory/Secretory products of adult fluke (Fh-ES). We also observed greater changes in gene expression in LPS activated BOMA cells than in non activated BOMA cells upon stimulation using Fh-ES. The results show possibility of using cell lines for in vitro research of bovine immune response against liver fluke, although this model still requires validation and further characterization.

Słowa kluczowe

EN

Fasciola hepatica; immune response; antigen; liver fluke; infection; interferon gamma; interleukin 4; tumour necrosis factor-alpha; gene expression

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 4
• Opis fizyczny: p.453-462,fig.,ref.

Twórcy

autor

Baska P.

• Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Zawistowska-Deniziak A.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

autor

Zdziarska A. M.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Wasyl K.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Wisniewski M.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Cywinska A.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 159C Nowoursynowska Street, 02-776 Warsaw, Poland

autor

Klockiewicz M.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences – SGGW, 8 Ciszewskiego Street, 02-786 Warsaw, Poland

autor

Januszkiewicz K.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

autor

Wedrychowicz H.

• Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland

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