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2018 | 67 | 2 |

Tytuł artykułu

The usefulness of chromogenic media for qualitative and semi-quantitative diagnostic of urinary tract infections

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to evaluate the usefulness of chromogenic media for isolation of bacteria from urine and direct identification of UTI pathogens. A total of 100 urine specimens were inoculated on blood agar and MacConkey agar as a reference method and on the following media to be tested: chromID® CPS® Elite (CPSE, bioMérieux), CHROMagarTM Orientation (BioMaxima), BD CHROMagar Orientation Medium (ORI, Becton Dickinson), CHROMagarTM Orientation (ORIE, Graso) and Brillance UTI Clarity Agar (UTI C, Oxoid). After a 24-hour incubation period, 47 Gram-positive cocci and 62 Gram-negative rods were observed. The specificity and sensitivity of all chromogenic media was 97.3% and 93.5% respectively for qualitative diagnostic; and 81.9% and 81.3% respectively for semi-quantitative diagnostic. The mean PPV and NPV of the chromogenic media were 98.7% and 87.7% for qualitative UTI diagnostic, and 90.9% and 71.9% respectively for semi-quantitative diagnostic.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

67

Numer

2

Opis fizyczny

p.213-218,ref.

Twórcy

  • Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland

Bibliografia

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  • Aspevall O., B. Osterman, R. Dittmer, L. Stén, E. Lindbäck and U. Forsum. 2002. Performance of four chromogenic urine culture media after one or two days of incubation compared with reference media. J. Clin. Microbiol. 40: 1500–1503.
  • Chan W.W. 2016. Urine cultures, pp. 3.12–1–3.12–2. In: Leber A.L. (ed.). Clinical microbiology procedures handbook. ASM Press, Washington, DC.
  • Chang J.C., M.L. Chien, H.M. Chen, J.J. Yan and J.J. Wu. 2008. Comparison of CPS ID 3 and CHROMagar Orientation chromogenic agars with standard biplate technique for culture of clinical urine samples. J. Microbiol. Immunol. Infect. 41: 422–427.
  • Fallon D., G. Ackland, N. Andrews, D. Frodsham, S. Howe, K. Howells, K.J. Nye and R.E. Warren. 2003. A comparison of the performance of commercially available chromogenic agars for the isolation and presumptive identification of organisms from urine. J. Clin. Pathol. 56: 608–612.
  • Filius P.M.G., D. van Netten, P.J.E. Roovers, A.G. Vulto, I.C. Gyssens, H.A. Verbrugh and H.P. Endtz. 2003. Comparative evaluation of three chromogenic agars for detection and rapid identification of aerobic Gram-negative bacteria in the normal intestinal microflora. Clin. Microbiol. Infect. 9: 912–918.
  • Flores-Mireles A.L., J.N. Walker, M. Caparon and S.J. Hultgren. 2015. Urinary tract infections: epidemiology, mechanisms of infection, and treatment options. Nat. Rev. Microbiol. 13: 269–284.
  • Green L.H. 2009. Culturing and Preserving Microorganisms, pp. 31–35. In: Goldman E. and L.H. Green (eds). Practical Handbook of Microbiology. CRC Press, Boca Raton, FL.
  • Jolkkonen S., E.L. Paattiniemi, P. Kärpänoja and H. Sarkkinen. 2010. Screening of urine samples by flow cytometry reduces the need for culture. J. Clin. Microbiol. 48: 3117–3121.
  • Kuch A., E. Stefaniuk, T. Ozorowski and W. Hryniewicz. 2009. New selective and differential chromogenic agar medium, chromID VRE, for screening vancomycin-resistant Enterococcus species.J. Microbiol. Methods. 77: 124–126.
  • Ojanen T., S. Kiiskinen, Y. Björkman, H. Laitinen, M.J. Virtanen and A. Siitonen. 2016. Use of external quality assessment to evaluate the reliability of quantitative urine culture. Arch. Clin. Microbiol. 7: 2–7.
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  • Rigaill J., P.O. Verhoeven, C. Mahinc, M. Jeraiby, F. Grattard, N. Fonsale, B. Pozzetto and A. Carricajo. 2015. Evaluation of new bioMérieux chromogenic CPS media for detection of urinary tract pathogens. J. Clin. Microbiol. 53: 2701–2702.
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  • Sharp S.E. and R.B. Clark (eds). 2009. Cumitech 31A,Verification and validation of procedures in the Clinical Microbiology Laboratory. ASM Press. Washington, DC.
  • Stefaniuk E., A. Baraniak, M. Fortuna, W. Hryniewicz. 2016. Usefulness of CHROMagar Candida medium, biochemical methods – API ID32C and VITEK 2 Compact and two MALDI-TOF MS Systems for Candida spp. identification. Polish J. Microbiol. 65: 111–114.
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  • Strauss S. and P.P. Bourbeau. 2015. Impact of introduction of the BD Kiestra InoquIA on urine culture results in a hospital clinical microbiology laboratory. J. Clin. Microbiol. 53: 1736–1740.
  • Yarbrough M.L., M.A. Wallace, C. Marshall, E. Mathias andA.-A.D. Burnham. 2016. Culture of urine specimens by use of chromID CPS Elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory. J. Clin. Microbiol. 54: 2767–2773.

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Bibliografia

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