The effects of grafting on glycolysis and the tricarboxylic acid cycle in Ca(NO3)2-stressed cucumber seedlings with pumpkin as rootstock

• Autorzy: Li L. , Xing W.-w. , Shao Q.-s. , Shu S. , Sun J. , Guo S.-r.
• Języki publikacji: EN

Abstrakty

EN

In this research, we investigated the effects of grafting on intermediate metabolites and key enzymes of glycolysis and the tricarboxylic acid (TCA) cycle in selfgrafted and salt-tolerant pumpkin rootstock-grafted cucumber seedlings supplied with nutrient solution and subjected to 80 mM Ca(NO₃)₂ stress for 6 days. Ca(NO₃)₂ stress induced accumulation of 3-phosphoglycerate (3- PGA) and phosphoenolpyruvate (PEP) in the leaves of selfgrafted cucumber seedlings and enhanced the activities of phosphoenolpyruvate carboxylase (PEPC) and enolase (ENO). Succinic acid and malic acid contents and isocitrate dehydrogenase, succinate dehydrogenase (SDH), and malate dehydrogenase (MDH) activities in self-grafted seedlings were significantly decreased by Ca(NO₃)₂ stress. In addition, activities of PEPC, ENO, SDH, and MDH and contents of glycolysis intermediate metabolites (citric, succinic, and malic acids) were significantly higher in leaves of rootstock-grafted seedlings compared with those in self-grafted seedlings under saline conditions. Furthermore, leaf adenosine triphosphate (ATP) content of rootstock- grafted seedlings was relatively higher than that in self-grafted plants under salt stress, with an opposite effect observed on adenosine diphosphate content. These results indicate that rootstock grafting alleviates Ca(NO₃)₂ stressinduced inhibition of the glycolytic pathway and the TCA cycle in cucumber seedling leaves, which may aid the respiratory metabolism of cucumber seedlings and help maintain a high ATP synthesis level, thereby increasing the biomass of cucumber seedlings and enhancing their salt tolerance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 12
• Opis fizyczny: Article: 259 [10 p.], fig.,ref.

Twórcy

autor

Li L.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

autor

Xing W.-w.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

autor

Shao Q.-s.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

autor

Shu S.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

autor

Sun J.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China
• Facility Horticulture Institute, Nanjing Agricultural University, Suqian, 223800, Jiangsu, China

autor

Guo S.-r.

• Key Laboratory of Southern Vegetables Genetic Improvement of Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China
• Facility Horticulture Institute, Nanjing Agricultural University, Suqian, 223800, Jiangsu, China

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Identyfikatory

DOI

10.1007/s11738-015-1978-5