PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 32 | 5 |

Tytuł artykułu

Ectopic overexpression of Arabidopsis AtmiR393a gene changes auxin sensitivity and enhances salt resistance in tobacco

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
To characterize the biological function of microRNA miR393 in tobacco, AtmiR393a gene was isolated from Arabidopsis using PCR and fused downstream to CaMV 35S promoter to make a plant expression construct 35S::AtmiR393a. The resultant construct was then introduced into tobacco with Agrobacterium-mediated transformation. Transgenic tobacco lines ectopically overexpressing AtmiR393a were successfully obtained. Transgenic lines L1 (a weak line), L2 (a middle line), and L3 (a strong line) were confirmed using stem-loop RT-PCRs and used to characterize the function of miR393 in tobacco. The results showed that L1, L2, and L3 exhibited reduced plant size and root length related to the WT control. In addition, seedling growth was less sensitive to IAA treatment and NaCl stress in three transgenic lines than the non-transgenic WT control. Furthermore, L1, L2, and L3 showed reduced phototropism relative to WT. Therefore, the biological function of miR393 is conserved in tobacco, just like in Arabidopsis. It regulates plant growth and development as well as the responses to environmental cues by influencing auxin sensitivity.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

32

Numer

5

Opis fizyczny

p.997-1003,fig.,ref.

Twórcy

autor
  • State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
autor
  • State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
autor
  • State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
autor
  • State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China
autor
  • State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, Shandong, China

Bibliografia

  • Brodersen P, Voinnet O (2009) Revisiting the principles of microRNA target recognition and mode of action. Nature 10:141–148
  • Buhtz A, Springer F, Chappell L, Baulcombe DC, Kehr J (2008) Identification and characterization of small RNAs from the phloem of Biassica napus. Plant J 53:739–749
  • Chen PY, Wang CK, Soong SC, To KY (2003) Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breed 11:287–293
  • Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33(20):e179
  • Dharmasiri N, Dharmasiri S, Estelle M (2005) The F-box protein TIR1 is an auxin receptor. Nature 435:441–445
  • Ding X, Cao Y, Huang L, Zhao J, Xu C, Li X, Wang S (2008) Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansion expression and promotes salicylate- and jasmonate-independent basal immunity in rice. Plant Cell 20:228–240
  • Guo H, Xie Q, Fei J, Chua N (2005) MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development. Plant Cell 17:1376–1386
  • Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
  • Jones-Rhoades MW, Bartel DP (2004) Computational identification of plant microRNAs and their targets, including a stress induced miRNA. Cell 14:787
  • Jones-Rhoades MW, Bartel DP, Bartel B (2006) MicroRNAs and their regulatory roles in plants. Curr Opin Plant Biol 57:19–53
  • Mallory AC, Bartel DP, Bartelc B (2005) MicroRNA-directed regulation of arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early Auxin response genes. Plant Cell 17:1360–1375
  • Mathieu J, Yant LJ, Mürdter F, Küttner F, Schmid M (2009) Repression of flowering by the miR172 target SMZ. PLoS Boil 7(7):e1000148
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jonathan DG (2006) A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science 312:436–439
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
  • Shukla LI, Chinnusamy V, Sunkar R (2008) The role of microRNAs and other endogenous small RNAs in plant stress responses. Biochim Biophys Acta 1779:743–748
  • Sunkar R, Chinnusamy V, Zhu J, Zhu JK (2007) Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci 12:301–309
  • Vanneste S, Friml J (2009) Auxin: a trigger for change in plant development. Cell 136:1005–1016
  • Wang J, Wang L, Mao Y, Cai W, Xue H, Chen X (2005) Control of root cap formation by microRNA-targeted auxin response factors in Arabidopsis. Plant Cell 17:2204–2216
  • Zhou X, Wang G, Sutoh K, Zhu JK, Zhang W (2008) Identification of cold-inducible miroRNAs in plants by transcriptome analysis. Biochim Biophys Acta 1779:780–788

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-8a9ffe25-d6a4-4a00-9fcc-a9f6ffd12581
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.