Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran

• Autorzy: Sarkari B. , Mansouri M. , Khabisi S.A. , Mowlavi G.
• Języki publikacji: EN

Abstrakty

EN

This study presents the first molecular and serological evaluation of Echinococcus granulosus infections in wild boars in Iran. Twenty five wild boars were collected in south-western Iran, during authorized hunting program, from March to October 2013, necropsied and examined for E. granulosus infection. Furthermore, seroprevalence of cystic echinococcosis in hunted boars was evaluated by an ELISA system. A fertile hydatid cyst due to E. granulosus was detected in the lung of one of the animals. Genotype analysis of the isolate was determined by analyzing a mitochondrial gene, cytochrome C oxidase subunit 1 (co1). DNA was extracted from the cyst sample and polymerase chain reaction amplification and DNA sequencing of the specific region of the co1 gene was performed. Molecular evaluation confirmed the presence of a sheep strain, the G1 genotype, in the wild boar in south-western Iran. This is the first report of the presence of G1 genotype of E. granulosus in wild boar in Iran. Serological evaluation of hydatid cyst by antigen-B ELISA revealed E. granulosus antibodies in 5 (20%) of 25 wild boars. A statistically significant difference was observed between the prevalence of E. granulosus antibodies and gender while the difference between the seroprevalence of E. granulosus and age was insignificant. Findings of this study might have important implications for the prevention and control of cystic echinococcosis.

Słowa kluczowe

EN

molecular characteristics; seroprevalence; Echinococcus granulosus; wild boar; genotype; Sus scrofa; Iran

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2015
• Tom: 61
• Numer: 4
• Opis fizyczny: p.269–273,fig.,ref.

Twórcy

autor

Sarkari B.

• Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
• Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

autor

Mansouri M.

• Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

autor

Khabisi S.A.

• Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

autor

Mowlavi G.

• Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Bibliografia

• [1] Eckert J., Deplazes P. 2004. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clinical Microbiology Reviews 17: 107-135.
• [2] Sadjjadi S.M. 2006. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitology International 55: S197-S202.
• [3] Sarkari B., Sadjjadi S.M., Beheshtian M.M., Aghaee M., Sedaghat F. 2010. Human cystic echinococcosis in Yasuj District in Southwest of Iran: an epidemiological study of seroprevalence and surgical cases over a ten-year period. Zoonoses and Public Health 57: 146-150.
• [4] Eslami A., Farsad-Hamdi S. 1992. Helminth parasites of wild boar, Sus scrofa, in Iran. Journal of Wildlife Diseases 28: 316-318.
• [5] Foata J., Culioli J.L., Marchand B. 2005. Helminth fauna of wild boar in Corsica. Acta Parasitologica 50: 168-170.
• [6] Martin-Hernando M.P., Gonzalez L.M., Ruiz-Fons F., Garate T., Gortazar C. 2008. Massive presence of Echinococcus granulosus (Cestoda, Taeniidae) cysts in a wild boar (Sus scrofa) from Spain. Parasitology Research 103: 705-707.
• [7] Senlik B., Cirak V.Y., Girisgin O., Akyol C.V. 2011. Helminth infections of wild boars (Sus scrofa) in the Bursa province of Turkey. Journal of Helminthology 85: 404-408.
• [8] Sanchez E., Caceres O., Naquira C., Miranda E., Samudio F., Fernandes O. 2012. Echinococcus granulosus genotypes circulating in alpacas (Lama pacos) and pigs (Sus scrofa) from an endemic region in Peru. Memórias do Instituto Oswaldo Cruz 107: 275-278.
• [9] Onac D., Gyorke A., Oltean M., Gavrea R., Cozma V. 2013. First detection of Echinococcus granulosus G1 and G7 in wild boars (Sus scrofa) and red deer (Cervus elaphus) in Romania using PCR and PCRRFLP techniques. Veterinary Parasitology 193: 289-291.
• [10] Solaymani-Mohammadi S., Mobedi I., Rezaian M., Massoud J., Mohebali M., Hooshyar H., Ashrafi K., Rokni M.B. 2003. Helminth parasites of the wild boar, Sus scrofa, in Luristan province, western Iran and their public health significance. Journal of Helminthology 77: 263-267.
• [11] Umhang G., Richomme C., Hormaz V., Boucher J.M., Boue F. 2014. Pigs and wild boar in Corsica harbor Echinococcus canadensis G6/7 at levels of concern for public health and local economy. Acta Tropica 133: 64-68.
• [12] Kedra A., Tkach V.V., Swiderski Z., Pawlowski Z., Emets A., Pawlowski J. 2000. Molecular characterisation of Echinococcus granulosus from a wild boar. Acta Parasitologica 45: 121-122.
• [13] Bowles J., Blair D., McManus D.P. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54:165-73.
• [14] Sadjjadi S.M., Abidi H., Sarkari B., Izadpanah A., Kazemian S. 2007. Evaluation of enzyme linked immunosorbent assay, utilizing native antigen B for serodiagnosis of human hydatidosis. Iran Journal of Immunology 4: 167-172.
• [15] Sarkari B., Sadjjadi S.M., Abidi H., Izadpanah A., Kazemian S., Rafati A. 2007. Application of western blotting using native antigen B for serodiagnosis of human cystic echinococcosis. Iranian Journal of Parasitology 2: 7-12.
• [16] Mohammadzadeh T., Sako Y., Sadjjadi S. M., Sarkari B., Ito A. 2012. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 106: 371-375.
• [17] McManus D.P. 2013. Current status of the genetics and molecular taxonomy of Echinococcus species. Parasitology 140: 1617-1623.
• [18] Nikmanesh B., Mirhendi H., Ghalavand Z., Alebouyeh M., Sharbatkhori M., Kia E., Mohebali M., Eghbali M., Rokni M.B. 2014. Genotyping of Echinococcus granulosus isolates from human clinical samples based on sequencing of mitochondrial genes in Iran, Tehran. Iranian Journal of Parasitology 9: 20-27.
• [19] Daniel M.K., Ponce-Gordo F., Cuesta-Bandera C. 2004. Genetic identification and host range of the Spanish strains of Echinococcus granulosus. Acta Tropica 91: 87-93.

Identyfikatory

DOI

10.17420/ap6104.18