Loureirin B inhibits the proliferation of hepatic stellate cells and the Wnt/β-catenin signaling pathway by regulating miR-148-3p

• Autorzy: Hu J. , Zhang R. , Tang M. , Li Y.
• Języki publikacji: EN

Abstrakty

EN

Background: We investigated the activity of loureirin B against liver fibrosis and the underlying molecular mechanisms. Methods: Hepatic stellate cells (HSCs) from Sprague-Dawley rats were treated with different concentrations of loureirin B. We used the MTT assay to determine HSC proliferation, flow cytometry to analyze apoptosis, and western blot to determine the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Real-time PCR was used to determine the expressions of Wnt1 and miR-148-3p. Results: The MTT assay showed that loureirin B treatment significantly inhibited the proliferation of HSCs in time- and dose-dependent manners. Loureirin B significantly promoted the apoptosis of HSCs, increased the expression of Bax and decreased the Bcl-2 level. Western blot analysis showed that the expressions of Wnt1 and β-catenin were obviously lower in the loureirin B treatment group than in the control group. We also found that loureirin B could decrease the Wnt1 mRNA level and increase miR-148-3p expression. Knockdown of miR-148-3p using inhibitor could reverse the effects of loureirin B on the proliferation and apoptosis of HSCs and the expressions of Bax, Bcl-2, Wnt1 and β-catenin. Conclusion: Our results suggest that loureirin B inhibited the proliferation and promoted the apoptosis of HSCs, and suppressed the Wnt/β-catenin signaling pathway via regulation of miR-148-3p.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-10,fig.,ref.

Twórcy

autor

Hu J.

• Department of Gastroenterology, the First People’s Hospital of Yunnan province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan province, China

autor

Zhang R.

• Department of Gastroenterology, the First People’s Hospital of Yunnan province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan province, China

autor

Tang M.

• Department of Gastroenterology, the First People’s Hospital of Yunnan province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan province, China

autor

Li Y.

• Department of Gastroenterology, the First People’s Hospital of Yunnan province, the Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan province, China

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Identyfikatory

DOI

10.1186/s11658-018-0098-9