Fractal and stereological analyses of insulin - induced rat exocrine pancreas remodelling

• Autorzy: Pajevic M. , Aleksic M. , Golic I. , Markelic M. , Otasevic V. , Jankovic A. , Stancic A. , Korac B. , Korac A.
• Języki publikacji: EN

Abstrakty

EN

Background: The effect of insulin on the endocrine pancreas has been the subject of extensive study, but quantitative morphometric investigations of the exocrine pancreas are scarce. This study was therefore undertaken to investigate the effect of acute and chronic insulin administration (two doses, 0.4 IU and 4 IU) on the morphology of rat pancreas acini. Materials and methods: Semi-fine sections stained with methylene blue and basic fuchsine or haematoxylin and eosin-stained 5-micrometer thick paraffin sections were used for fractal and stereological analysis of exocrine acini. Acute insulin treatment, independent of applied doses increased fractal dimension in line with decreased lacunarity of pancreas acini. Chronic low dose insulin decreased fractal dimension and increased lacunarity of pancreas acini, but a high dose had the opposite effect. The volume densities (Vv) of cytoplasm, granules and nucleus are affected differently: acute low dose and high chronic dose significantly decreased granules Vv, and in line increased cytoplasmic Vv, whereas other examined structures showed slight changes without statistical significance. Results: The results obtained from this investigation indicate that insulin treatment induced structural remodelling of the exocrine pancreas suggesting a substantial role of insulin in its functioning. Conclusions: Additionally, we showed that fine architectural changes in acini could be detected by fractal analysis, suggesting this method as an alternative or addition to routine stereology. (Folia Morphol 2018; 77, 3: 478–484)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2018
• Tom: 77
• Numer: 3
• Opis fizyczny: p.478–484,fig.,ref.

Twórcy

autor

Pajevic M.

• Department of Histopathology, Clinical Centre of Serbia, Belgrade, Serbia

autor

Aleksic M.

• Center for Electron Microscopy, University of Belgrade, Faculty of Biology, Belgrade, Serbia

autor

Golic I.

• Center for Electron Microscopy, University of Belgrade, Faculty of Biology, Belgrade, Serbia

autor

Markelic M.

• Center for Electron Microscopy, University of Belgrade, Faculty of Biology, Belgrade, Serbia

autor

Otasevic V.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia

autor

Jankovic A.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia

autor

Stancic A.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia

autor

Korac B.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia

autor

Korac A.

• Center for Electron Microscopy, University of Belgrade, Faculty of Biology, Belgrade, Serbia

Bibliografia

• 1. Al-Mrabeh A, Hollingsworth KG, Steven S, et al. Morphology of the pancreas in type 2 diabetes: effect of weight loss with or without normalisation of insulin secretory capacity. Diabetologia. 2016; 59(8): 1753–1759, doi: 10.1007/s00125-016-3984-6, indexed in Pubmed: 27179658.
• 2. Ashcroft FM, Rorsman P. Diabetes mellitus and the b cell: the last ten years. Cell. 2012; 148(6): 1160–1171, doi: 10.1016/j.cell.2012.02.010, indexed in Pubmed: 22424227.
• 3. Aughsteen AA, Mohammed FI. Insulin enhances amylase and lipase activity in the pancreas of streptozotocin-diabetic rats. An in vivo study. Saudi Med J. 2002; 23(7): 838–844, indexed in Pubmed: 12174237.
• 4. Bani D, Magnani L, Sacchi TB, et al. The exocrine pancreas in patients with hyperinsulinemic hypoglycemia. A morphometrical and ultrastructural study. Int J Pancreatol. 1989; 5(3): 239–248, indexed in Pubmed: 2550561.
• 5. Barreto SG, Carati CJ, Toouli J, et al. The islet-acinar axis of the pancreas: more than just insulin. Am J Physiol Gastrointest Liver Physiol. 2010; 299(1): G10–G22, doi: 10.1152/ajpgi.00077.2010, indexed in Pubmed: 20395539.
• 6. Bizzarri M, Giuliani A, Cucina A, et al. Fractal analysis in a systems biology approach to cancer. Semin Cancer Biol. 2011; 21(3): 175–182, doi: 10.1016/j.semcancer.2011.04.002, indexed in Pubmed: 21514387.
• 7. Blanco-Molina A, González-Reyes JA, Torre-Cisneros J, et al. Effects of hypothyroidism on the ultrastructure of rat pancreatic acinar cells: a stereological analysis. Histol Histopathol. 1991; 6(1): 37–42, indexed in Pubmed: 1806054.
• 8. Campbell-Thompson M, Rodriguez-Calvo T, Battaglia M. Abnormalities of the exocrine pancreas in type 1 diabetes. Curr Diab Rep. 2015; 15(10): 79, doi: 10.1007/s11892-015-0653-y, indexed in Pubmed: 26318606.
• 9. Cano DA, Hebrok M, Zenker M. Pancreatic development and disease. Gastroenterology. 2007; 132(2): 745–762, doi: 10.1053/j.gastro.2006.12.054, indexed in Pubmed: 17258745.
• 10. Cordain L, Eades MR, Eades MD. Hyperinsulinemic diseases of civilization: more than just Syndrome X. Comp Biochem Physiol A Mol Integr Physiol. 2003; 136(1): 95–112, indexed in Pubmed: 14527633.
• 11. Corkey BE. Banting lecture 2011: hyperinsulinemia: cause or consequence? Diabetes. 2012; 61(1): 4–13, doi: 10.2337/db11-1483, indexed in Pubmed: 22187369.
• 12. Ferro DP, Falconi MA, Adam RL, et al. Fractal characteristics of May-Grünwald-Giemsa stained chromatin are independent prognostic factors for survival in multiple myeloma. PLoS One. 2011; 6(6): e20706, doi: 10.1371/journal.pone.0020706, indexed in Pubmed: 21698234.
• 13. Gheonea DI, Streba CT, Vere CC, et al. Diagnosis system for hepatocellular carcinoma based on fractal dimension of morphometric elements integrated in an artificial neural network. Biomed Res Int. 2014; 2014: 239706, doi: 10.1155/2014/239706, indexed in Pubmed: 25025042.
• 14. Gonzalez E, Flier E, Molle D, et al. Hyperinsulinemia leads to uncoupled insulin regulation of the GLUT4 glucose transporter and the FoxO1 transcription factor. Proc Natl Acad Sci U S A. 2011; 108(25): 10162–10167, doi: 10.1073/pnas.1019268108, indexed in Pubmed: 21646544.
• 15. Gudea AI, Stefan AC. Histomorphometric, fractal and lacunarity comparative analysis of sheep (Ovis aries), goat (Capra hircus) and roe deer (Capreolus capreolus) compact bone samples. Folia Morphol. 2013; 72(3): 239–248, indexed in Pubmed: 24068686.
• 16. Hamamoto N, Ashizawa N, Niigaki M, et al. Morphological changes in the rat exocrine pancreas after pancreatic duct ligation. Histol Histopathol. 2002; 17(4): 1033–1041, doi: 10.14670/HH-17.1033, indexed in Pubmed: 12371130.
• 17. Hayden MR, Patel K, Habibi J, et al. Attenuation of endocrineexocrine pancreatic communication in type 2 diabetes: pancreatic extracellular matrix ultrastructural abnormalities. J Cardiometab Syndr. 2008; 3(4): 234–243, doi: 10.1111/j.1559-4572.2008.00024.x, indexed in Pubmed: 19040593.
• 18. Hotar V, Salac P. Surface evaluation by estimation of fractal dimension and statistical tools. Sci World J. 2014; 2014: 435935, doi: 10.1155/2014/435935, indexed in Pubmed: 25250380.
• 19. Jo J, Hörnblad A, Kilimnik G, et al. The fractal spatial distribution of pancreatic islets in three dimensions: a selfavoiding growth model. Phys Biol. 2013; 10(3): 036009, doi: 10.1088/1478-3975/10/3/036009, indexed in Pubmed: 23629025.
• 20. Johnson JD, Alejandro EU. Control of pancreatic beta-cell fate by insulin signaling: The sweet spot hypothesis. Cell Cycle. 2008; 7(10): 1343–1347, doi: 10.4161/cc.7.10.5865, indexed in Pubmed: 18418065.
• 21. Mehran AE, Templeman NM, Brigidi GS, et al. Hyperinsulinemia drives diet-induced obesity independently of brain insulin production. Cell Metab. 2012; 16(6): 723–737, doi: 10.1016/j.cmet.2012.10.019, indexed in Pubmed: 23217255.
• 22. Metze K. Fractal dimension of chromatin: potential molecular diagnostic applications for cancer prognosis. Expert Rev Mol Diagn. 2013; 13(7): 719–735, doi: 10.1586/14737159.2013.828889, indexed in Pubmed: 24063399.
• 23. Motta PM, Macchiarelli G, Nottola SA, et al. Histology of the exocrine pancreas. Microsc Res Tech. 1997; 37(5-6): 384–398, doi: 10.1002/(SICI)1097-0029(19970601)37:5/6<384::AIDJEMT3>3.0.CO;2-E, indexed in Pubmed: 9220418.
• 24. Pantic I, Nesic D, Stevanovic D, et al. Effects of ghrelin on the structural complexity of exocrine pancreas tissue architecture. Microsc Microanal. 2013; 19(3): 553–558, doi: 10.1017/S1431927613000524, indexed in Pubmed: 23628379.
• 25. Pap A. Effects of insulin and glucose metabolism on pancreatic exocrine function. Int J Diabetes Metab. 2004; 12: 30–34.
• 26. Piciucchi M, Capurso G, Archibugi L, et al. Exocrine pancreatic insufficiency in diabetic patients: prevalence, mechanisms, and treatment. Int J Endocrinol. 2015; 2015: 595649, doi: 10.1155/2015/595649, indexed in Pubmed: 25892991.
• 27. Rhodes CJ, White MF. Molecular insights into insulin action and secretion. Eur J Clin Invest. 2002; 32 Suppl 3: 3–13, indexed in Pubmed: 12028370.
• 28. Saito A, Williams JA, Kanno T. Potentiation of cholecystokinin-induced exocrine secretion by both exogenous and endogenous insulin in isolated and perfused rat pancreata. J Clin Invest. 1980; 65(4): 777–782, doi: 10.1172/JCI109727, indexed in Pubmed: 6987265.
• 29. Samuel VT, Shulman GI. Mechanisms for insulin resistance: common threads and missing links. Cell. 2012; 148(5): 852–871, doi: 10.1016/j.cell.2012.02.017, indexed in Pubmed: 22385956.
• 30. Shanik MH, Xu Y, Skrha J, et al. Insulin resistance and hyperinsulinemia: is hyperinsulinemia the cart or the horse? Diabetes Care. 2008; 31 Suppl 2: S262–S268, doi: 10.2337/dc08-s264, indexed in Pubmed: 18227495.
• 31. Sirri R, Bianco C, De Vico G, et al. Proliferation, apoptosis, and fractal dimension analysis for the quantification of intestinal trophism in sole (Solea solea) fed mussel meal diets. BMC Vet Res. 2014; 10: 148, doi: 10.1186/1746-6148-10-148, indexed in Pubmed: 24997003.
• 32. Slavin BG, Zarow C, Warden CH, et al. Histological, immunocytochemical, and morphometrical analyses of pancreatic islets in the BSB mouse model of obesity. Anat Rec (Hoboken). 2010; 293(1): 108–116, doi: 10.1002/ar.21019, indexed in Pubmed: 19798700.
• 33. Taga R, Faverão MR, Cestari TM, et al. Morphometric dimensions of Syrian golden hamster pancreas of both sexes. Okajimas Folia Anat Jpn. 1999; 76(1): 41–46, indexed in Pubmed: 10409844.
• 34. Weibel ER, Kistler GS, Scherle WF. Practical stereological methods for morphometric cytology. J Cell Biol. 1966; 30(1): 23–38, indexed in Pubmed: 5338131.
• 35. West BJ. Fractal physiology and the fractional calculus: a perspective. Front Physiol. 2010; 1: 12, doi: 10.3389/fphys.2010.00012, indexed in Pubmed: 21423355.
• 36. Wilcox G. Insulin and insulin resistance. Clin Biochem Rev. 2005; 26(2): 19–39.
• 37. Williams JA, Goldfine ID. The insulin-pancreatic acinar axis. Diabetes. 1985; 34(10): 980–986, indexed in Pubmed: 2412919.
• 38. Williams JA. Regulation of acinar cell function in the pancreas. Curr Opin Gastroenterol. 2010; 26(5): 478–483, doi: 10.1097/ MOG.0b013e32833d11c6, indexed in Pubmed: 20625287.
• 39. Yang X, Mei S, Gu H, et al. Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol. 2014; 221(3): 469–480, doi: 10.1530/JOE-14-0117, indexed in Pubmed: 24741073.

Identyfikatory

DOI

10.5603/FM.a2017.0106