Determination of total mercury in fish and Cormorant using cold vapour atomic absorption spectrometry

• Autorzy: Konieczka P. , Misztal-Szkudlinska M. , Namiesnik J. , Szefer P.
• Języki publikacji: EN

Abstrakty

EN

One of the most frequently used methods for determining total mercury content in biological samples is cold vapour atomic absorption spectrometry (CV-AAS), which is extensively used in the biomonitoring of environmental pollution. Seabirds are often used as bioindicators of metal contamination because of their specific feeding habits, wide geographical ranges and long lifespan. This paper describes the validation of CV-AAS for determining the total mercury content in biological samples (whole fish, cormorant tissues). The development and optimization of the procedure is outlined, and the main objective of this study was to calculate its validation parameters. The selectivity of the method was documented; linearity (r>0.993) ranged from 0.29 to 100 ng of total mercury per sample mass. For a total Hg content of 80-1,000 ng, a polynomial calibration curve derived directly the Lambert-Beer law was used. The method showed good recoveries (average 98.0%) and a relative standard deviation for repeatability of < 10%. The limit of detection was calculated at 0.096 ng of total Hg per sample mass. The uncertainty budget was evaluated according to the ‘Guide to the Expression of Uncertainty in Measurement’ (GUM) [1]; the relative expanded uncertainty was estimated at < 13%.

Słowa kluczowe

EN

determination; total mercury; mercury content; fish; Cormorant; cold vapour atomic absorption spectrometry; validation parameter

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2010
• Tom: 19
• Numer: 5
• Opis fizyczny: p.931-936,fig.,ref.

Twórcy

autor

Konieczka P.

• Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

autor

Misztal-Szkudlinska M.

• Department of Food Sciences, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland

autor

Namiesnik J.

• Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

autor

Szefer P.

• Department of Food Sciences, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland

Bibliografia

• 1. Guide to the Expression of Uncertainty in Measurement (GUM), ISO, Geneva 1993.
• 2. BOENING D. W. Ecological effects, transport, and fate of mercury: a general review. Chemosphere 40, 1335, 2000.
• 3. DOWNS S.G., MACLEOD C. L., LESTER J. N. Mercury in Precipitation and Its Relation to Bioaccumulation in Fish: A Literature Review. Water Air Soil Pollut. 108, 149, 1998.
• 4. BOSZKE L, SIEPAK J, FALANDYSZ J. Total mercury contamination of selected organisms in the Puck Bay, Baltic Sea, Poland. Pol. J. Environ. Stud. 12, (3), 275, 2003.
• 5. HOUSEROVÁ P., HEDBAVNY J., MATEJICEK D., KRACMAR S., SITKO J., KUBÁŇ V. Determination of total mercury in muscle, intestines, liver and kidney tissues of cormorant (Phalacrocorax carbo), great crested grebe (Podiceps cristatus) and Eurasian buzzard (Buteo buteo). Vet. Med. - Czech 50, (2), 61, 2005.
• 6. HOUSEROVÁ P., KUBÁŇ V., KRACMAR S., SITKO J. Total mercury and mercury species in birds and fish in an aquatic ecosystem in the Czech Republic. Environ. Pollut. 145, (1), 185, 2007.
• 7. KIM E. Y., SAEKI K., TANABE S., TANAKA H., TATSUKAWA R. Specific accumulation of mercury and selenium in seabirds. Environ. Pollut. 94, (3), 261, 1996.
• 8. NAM D. H., ANAN Y., IKEMOTO T., OKABE Y., KIM E.- Y., SUBRAMANIAN A., SAEKI K., TANABE S. Specific accumulation of 20 trace elements in great cormorants (Phalacrocorax carbo) from Japan. Environ. Pollut. 134, (3), 503, 2005.
• 9. SAEKI K., OKABE Y., KIM E.-Y., TANABE S., FUKUDA M., TATSUKAWA R. Mercury and cadmium in common cormorants (Phalacrocorax carbo). Environ. Pollut. 108, (2), 249, 2000.
• 10. NAMIEŚNIK J. Modern trends in monitoring and analysis of environmental pollutants. Pol. J. Environ. Stud. 10, (3), 127, 2001.
• 11. NAMIEŚNIK J. Trends in environmental analytics and monitoring. Crit. Rev. Anal. Chem. 30, 221, 2000.
• 12. KOT A., NAMIEŚNIK J. The role of speciation in analytical chemistry. Trends Anal. Chem. 19, 69, 2000.
• 13. SIEPAK J., BOSZKE L. Analytical methods in determination of mercury species in environmental samples; an overview. Acta Toxicologica. 12, (2) 87, 2004.
• 14. BOSZKE L., GŁOSIŃSKA G., SIEPAK J. Some aspects of speciation of mercury in a water environment. Pol. J. Environ. Stud. 11, (4), 285, 2002.
• 15. BOSZKE L., KOWALSKI A., GŁOSIŃSKA G., SZAREK R., SIEPAK J. Environmental factors affecting the speciation of mercury in the bottom sediments: an overview. Pol. J. Environ. Stud. 12, (1) 5, 2003.
• 16. KONIECZKA P. The role of the place of method validation in the quality assurance and quality control (QA/QC) system. Crit. Rev. Anal. Chem. 37, 173, 2007.
• 17. HUBER L. Validation and Qualification in Analytical Laboratories, www.labcompliance.com, 1998.
• 18. AMBRUS A. Practical Approach to Validation of Methods for Analysis of Residues, FAO/IAEA Training and Reference Centre for Food and Pesticide Control, Draft 1, http://www.iaea.or.at/programmes/nafa/d5/trcfpc/d5-trcfpc.html, 1999.
• 19. International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use: Text on Validation of Analytical Procedures, ICH-Q2A, Geneva 1994.
• 20. International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use: Validation of Analytical Procedures: Metrology, ICH-Q2B, Geneva 1996.
• 21. United States Pharmacopeial Convention, United States Pharmacopeia 23, US Rockville, 1995.
• 22. EURACHEM Guide: The Fitness for Purpose of Analytical Methods, First Internet Version, 1998.
• 23. International Vocabulary of Basic General Terms in Metrology, 3rd Edition, Draft April 2004, http://www.ncsli.org/vim/VIM_final_fd_13april041.pdf
• 24. Mercury Analysis System, “Mercury/MA-2000,” Instruction Manual, Nippon Instruments Corporation, No. NIC-600- 2009-04.
• 25. VOGELGESANG J., HÄDRICH J. Limits of detection, identification and determination: a statistical approach for practitioners. Accred. Qual. Assur. 6, 242, 1998.
• 26. JOHNSTON R. K., VALENTE R. M. Specifying and Evaluating Analytical Chemistry Quality Requirements for Ecological Risk Assessment in Marine Environmental Support Office, Technical Memorandum 99-01, San Diego, 2000.
• 27. Analytical Detection Limit Guidance, Wisconsin Department of Natural Resources, 1996.
• 28. KONIECZKA P., NAMIEŚNIK J. Quality Assurance and Quality Control in the Analytical Chemical Laboratory: A Practical Approach, CRC Press, Francis and Taylor, 2009.