Effects of medical plant extracts on the growth of the fish parasite Spironucleus vortens

• Autorzy: Puk K. , Guz L.
• Języki publikacji: EN

Abstrakty

EN

The economic importance of Spironucleus diseases led the authors to study the effect of aqueous and ethanol plant extracts on the growth of S. vortens. In the present study 0.025 g L⁻¹, 0.05 g L⁻¹ and 0.10 g L⁻¹ (w/v) aquatic and ethanol extracts of tetterwort (Chelidonium majus), purple coneflower (Echinacea purpurea), garlic (Allium sativum), chestnut (Aesculus hippocastanum), horseradish (Armoracia rusticana), Bryophyllum pinnatum (Kolanchoe pinnata), oregano (Origanum vulgare), tansy (Tanacetum vulgare), thyme (Thymus vulgaris), and yarrow (Achillea millefolium) were tested against in vitro growth of Spironucleus vortens isolated from the digestive tract of discus (Symphysodon discus). The extracts of chestnut, garlic, horseradish, oregano and tansy were found to be the most effective. The 0.10 g L⁻¹ extracts of these plants attained a high level of over 90% parasite growth inhibition, while their 0.025 g L⁻¹ extracts, with the exception of the oregano, attained 60% parasite growth inhibition. The results of the study confirmed that natural products are potential sources of new agents for the treatment and control of spironucleosis.

Słowa kluczowe

EN

medicinal plant; plant extract; growth; fish; animal parasite; parasite; Spironucleus vortens; ornamental fish

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 03
• Opis fizyczny: p.165-168,ref.

Twórcy

autor

Puk K.

• Institute of Biological Bases of Animal Diseases, Sub-department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland

autor

Guz L.

• Institute of Biological Bases of Animal Diseases, Sub-department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland

Bibliografia

• 1. Baker D. G., Malineni S., Taylor H. W.: Experimental infection of inbred mouse strains with Spironucleus muris. Vet. Parasitol. 1998, 77, 305-310.
• 2. Behnia M., Haghighi A., Komeylizadeh H., Tabaei S. J. S., Abadi A.: Inhibitory effects of Iranian Thymus vulgaris extracts on in vitro growth of Entamoeba histolytica. Korean J. Parasitol. 2008, 46, 153-156.
• 3. Bel’kova N. L., Dzyuba E. V., Sukhanova E. V.: Molecular-genetic detection of a nonpathogenic genotype of Spironucleus barkhanus (Diplomonadida: Hexamitidae) in the bleck Baikal grayling (Thymallus arcticus baicalensis Dybowski, 1874). Biology Bull. 2008, 35, 219-221.
• 4. Cooper G. L., Charlton B. R., Bickford A. A., Nordhausen R.: Hexamita meleagridis (Spironucleus meleagridis) infection in chukar partridges associated with high mortality and intracellular trophozoites. Avian Dis. 2004, 48, 706-710.
• 5. Edwards D. I.: Nitroimidazole drugs – action and resistance mechanisms. I. Mechanisms of action. J. Antimicrob. Chemother. 1993, 31, 9-20.
• 6. Guz L., Szczepaniak K.: Intestinal amoebiasis in Heckel discus Symphysodon discus – A case report. Bull. Eur. Ass. Fish Pathol. 2009, 29, 28-33.
• 7. Harris J. C., Plummer S., Turner M. P., Lloyd D.: The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology 2000, 146, 3119-3127.
• 8. Jørgensen A., Alfjorden A., Henriksen K., Sterud E.: Phylogenetic analysis of the SSU rRNA gene from the piscine diplomonad Spironucleus torosus (Diplomonadida: Hexamitinae). Folia Parasitol. 2007, 54, 277-282.
• 9. Jørgensen A., Sterud D.: The marine pathogenic genotype of Spironucleus barkhanus from farmed salmonids redescribed as Spironucleus salmonicida n. sp. J. Eukaryot. Microbiol. 2006, 53, 531-541.
• 10. Jørgensen A., Torp K., Bjørland M. A., Poppe T. T.: Wild Arctic char Salvelinus alpinus and trout Salmo trutta: hosts and reservoir of the salmonid pathogen Spironucleus salmonicida (Diplomonadida; Hexamitidae). Dis. Aquat. Org. 2011, 97, 57-63.
• 11. Keister D. B.: Axenic culture of Giardia lamblia in TYI-S- 33 medium supplemented with bile. Trans. R. Soc. Trop. Med. 1983, 77, 487-488.
• 12. Kolisko M., Cepicka I., Hampl V., Kulda J., Flegr J.: The phylogenetic position of enteromonads: a challenge for the present models of diplomonad evolution. Int. J. Syst. Evol. Microbiol. 2005, 55, 1729-1733.
• 13. Lanzky P. F., Halling-Sorensen B.: The toxic effect of the antibiotic metronidazole on aquatic organisms. Chemosphere 1997, 1, 2553-2561.
• 14. Lloyd D., Kristensen B.: Metronidazole inhibition of hydrogen production in vivo in drug-sensitive and resistant strains of Trichomonas vaginalis. J. Gen. Microbiol. 1985, 131, 849-853.
• 15. Lom J., Dykova I.: Protozoan Parasites in Fishes. Elsevier, Amsterdam 1992.
• 16. Luize P. S., Tiuman T. S., Morello L. G., Maza P. K., Ueda-Nakamura T., Filho B. P. D., Cortez D. A. G., deMello C. P., Nakamura C. V.: Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Braz. J. Pharm. Sci. 2005, 41, 85-95.
• 17. Lun Z. R., Burri C., Menzinger M., Kaminsky R.: Antiparasitic activity of diallyl trisulfide (Dasuansu) on human and animal pathogenic protozoa (Trypanosoma sp., Entamoeba histolytica and Giardia lamblia) in vitro. Ann. Soc. Belg. Med. Trop. 1994, 74, 51-59.
• 18. Millet C. O. M.: Growth, metabolism, ultrastructure and chemotherapy of Spironucleus vortens. PhD thesis, Cardiff University 2009.
• 19. Millet C. O. M., Lloyd D., Williams C., Williams D., Evans G., Saunders R. A., Cable J.: Effect of garlic and allium-derived products on the growth and metabolism of Spironucleus vortens. Exp. Parasitol. 2011, 127, 490-499.
• 20. Muelas-Serrano S., Nogal J. J., Martinez-Diaz R. A., Escario J. A., Martinez-Fernández A. R., Gómez-Barrio A.: In vitro screening of American plant extracts on Trypanosoma cruzi and Trichomonas vaginalis. J. Ethnopharmacol. 2000, 71, 101-107.
• 21. Murnigsih T., Subeki, Matsuura H., Takahashi K., Yamasaki M., Yamato O., Maede Y., Katakura K., Suzuki M., Kobayashi S., Chairul, Yoshihara T.: Evaluation of the inhibitory activities of the extracts of Indonesian traditional medicinal plants against Plasmodium falciparum and Babesia gibsoni. J. Vet. Med. Sci. 2005, 67, 829-831.
• 22. Muzitano M. F., Luzineide W. T., Catherine G., Kaiser C. R., Ross-Bergmann B., Costa S. S.: The antileishmanial activity assessment of unusual flavonoids from Kalanchoe pinnata. Phytochemistry 2006, 67, 2071-2077.
• 23. Nok A. J., Williams S., Onyenekwe P. C.: Allium sativum – induced death of African trypanosomes. Parasitol. Res. 1996, 82, 634-637.
• 24. Paull G. C., Matthews R. A.: Spironucleus vortens, a possible cause of hole-in-the-head disease in cichlids. Dis. Aquat. Org. 2001, 45, 197-202.
• 25. Payne M. A., Baynes R. E., Sundlof S. F., Craigmill A., Webb A. I., Riviere J. E.: Drugs prohibited from extralabel use in food animals. JAVMA 1999, 215, 28-32.
• 26. Poynton S. L., Fraser W., Francis-Floyd R., Rutledge P., Reed P., Nerad T. A.: Spironucleus vortens n. sp. from the freshwater angelfish Pterophyllum scalare morphology and culture. J. Eukaryot. Microbiol. 1995, 42, 731-742.
• 27. Rasoloson D., Tomkova E., Cammack R., Kulda J., Tachezy J.: Metronidazole resistant strains of Trichomonas vaginalis display increased sensitivity to oxygen. Parasitology 2001, 123, 45-56.
• 28. Sangmaneedet S., Smith S. A.: Efficacy of various chemotherapeutic agents on the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish. Dis. Aquat. Org. 1999, 38, 47-52.
• 29. Soudi S., Hashemi S. M., Hosseini A. Z., Ghaemi A., Jafarabadi M. A.: Antileishmanial effect of Echinacea purpurea root extract cultivated in Iran. Iran. J. Pharm. Res. 2000, 6, 147-149.
• 30. Sterud E., Mo T. A., Poppe T. T.: Systemic spironucleosis in sea-farmed Atlantic salmon Salmo salar, caused by Spironucleus barkhanus transmitted from feral Arctic char Salvelinus alpinus? Dis. Aquat. Org. 1998, 33, 63-66.
• 31. Sterud E., Poynton S. L.: Spironucleus vortens (Diplomonadida) in the ide, Leuciscus idus (L.) (Cyprinidae): a warm water hexamitid flagellatefound in north Europe. J. Eukaryot. Microbiol. 2002, 49, 137-145.
• 32. Tojo J. L., Santamarina M. T.: Oral pharmacological treatments for parasitic diseases of rainbow trout Oncorhynchus mykiss. I: Hexamita salmonis. Dis. Aquat. Org. 1998, 33, 51-56.
• 33. Treves-Brown K. M.: Availability of medicine for fish. Fish Vet. J. 1999, 4, 40-55.
• 34. Wassmann C., Hellberg A., Tannich E., Bruchhaus I.: Metronidazole resistance in the protozoan parasite Entamoeba histolytica is associated with increased expression of iron-containing superoxide dismutase and peroxiredoxin and decreased expression of ferredoxin 1 and flavin reductase. J. Biol. Chem. 1999, 274, 26051-26056.
• 35. Williams C. F., Lloyd D., Kolarich D., Alagesan K., Duchêne M., Cable J., Williams D., Leitsch D.: Disrupted intracellular redox balance of the diplomonad fish parasite Spironucleus vortens by 5-nitroimidazoles and garlic-derived compounds. Vet. Parasitol. 2012, 190, 62-73.
• 36. Williams C. F., Lloyd D., Poynton S. L., Joergensen A., Millet C. O. M., Cable J.: Spironucleus species: economically-important fish pathogens and enigmatic single-celled eukaryotes. J. Aquac. Res. Dev. 2011, 52, S2:002. doi:10.4172/2155-9546.S2-002.
• 37. Yao J. Y., Li X., Shen J., Pan X., Hao G., Xu Y., Ying W., Ru H., Liu X.: Isolation of bioactive components from Chelidonium majus L. with activity against Trichodina sp. Aquaculture 2011, 318, 235-238.