Isolation and fluorometric determination of homovanillic acid in urine and tissues

• Autorzy: Maruchin J.E. , Olesinski J.
• Języki publikacji: EN

Abstrakty

EN

Maruchin, J. E. and Olesiński, J.: Isolation and fluorometric determination of homovanillic acid in urine and tissues. Acta physiol, pol., 1988, 39 (2): 129-135. The fluorometric method is described for determination of the final product of dopamine metabolism homovanillic acid in urine, amniotic fluid and tissue extracts. The metabolit was isolated using three chromatographic columns (1-Amberlit CG-50, 2-Dowex AG 1X2, 8-Aluminium oxid). Oxidation of eluates to emitter was done with potassium ferricyanide in alkalic medium. The use of internal standards made high recovery of compound and a good specificity and sensitivity of detection.

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1988
• Tom: 39
• Numer: 2
• Opis fizyczny: p.129-135,fig.,ref.

Twórcy

autor

Maruchin J.E.

• Department of Biochemistry, Medical Center of Postgraduate Education, Warsaw, Poland

autor

Olesinski J.

• Department of Biochemistry, Medical Center of Postgraduate Education, Warsaw, Poland

Bibliografia

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