Petal: a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L.

• Autorzy: Mohammadi-Dehcheshmeh M. , Khalighi A. , Naderi R. , Sardari M. , Ebrahimie E.
• Języki publikacji: EN

Abstrakty

EN

Wild populations of Fritillaria imperialis L. are facing extinction and need urgent conservation. This paper presents an efficient system for in vitro direct bulblet regeneration of these populations by petal culturing of flower buds. Petals at different developmental stages, green-closed flower bud (before nectar secretion) and redclosed flower bud (beginning of nectar secretion), were used as explants, and the effects of various proportions of cytokinin to auxin on direct bulblet regeneration pathway were evaluated. More explants switched on direct regeneration pathway in combination of auxins (0.6 mg l⁻¹ NAA + 0.4 mg l⁻¹ IAA) with higher level of cytokinin (1 mg l⁻¹ BAP). In contrast, auxins (0.6 mg l⁻¹ NAA + 0.4 mg l⁻¹ IAA) with lower level of cytokinin (0.1 mg l⁻¹ BAP) produced more bulblets per regenerated explant. In green-closed flower bud stage, direct bulblets regenerated from the end of petal where it was connected to the receptacle, while nectar secretion site was the place of bulblet formation in red-closed flower bud stage. In addition, genotype-dependency of direct bulblet regeneration pathway was investigated by using two different wild populations of Fritillaria imperialis. This plant regeneration procedure was applicable to different Fritillaria genotypes and regenerated bulblets were normal.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 3
• Opis fizyczny: p.395-399,fig.,ref.

Twórcy

autor

Mohammadi-Dehcheshmeh M.

• Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, 31587-11167 Karaj, Iran

autor

Khalighi A.

• Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, 31587-11167 Karaj, Iran

autor

Naderi R.

• Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, 31587-11167 Karaj, Iran

autor

Sardari M.

• Agricultural and Natural Resources Research Center, Shahrekord, Chahar Mahal va Bakhtiari, Iran

autor

Ebrahimie E.

• Department of Crop Production and Plant Breeding, Faculty of Agriculture, Shiraz University, Shiraz, Iran

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