The influence of type and orientation of explants on in vitro growth and development of Cosmos atrosanguineus (Hook.) Voss

• Autorzy: Kozak D. , Pogroszewska E. , Szmagara M.

Warianty tytułu

PL

Wpływ rodzaju i sposobu ułożenia eksplantatu na wzrost i rozwój onętkaa krwistoczerwonego Cosmos atrosanguineus (Hook.) Voss in vitro

• Języki publikacji: EN

Abstrakty

EN

Cosmos atrosanguineus is a tuberous, tender perennial with velvety, dahlialike, brownish-red flowers that have a chocolate aroma. It is sterile, so no viable seeds are produced. This species is particularly suitable for a border plant in any perennial garden. A study was undertaken to determine whether shoot apex and leaf removal as well as explant orientation had an influence on axillary shoot induction. Explants were prepared from shoots taken from aseptically grown shoot cluster and were cultured for 6 weeks on Murashige and Skoog medium containing BA (1 mg·dm-3). The study results showed that the removal of the apex from the non-defoliated shoot tip improved axillary branching. The removal of developed leaves from the shoot tip with the apex removed caused a reduction in axillary shoot production. The defoliated shoot tip inserted vertically in the medium in an inverted position regenerated the highest number of axillary shoots characterized by the highest fresh weight. The defoliated shoot tip after removal of the apex, placed vertically with the base down, produced axillary shoots characterized by the greatest elongation growth and the highest percentage of shoots that reached a length of more than 1 cm.

PL

Cosmos atrosanguineus jest bulwiastą byliną wrażliwą na mróz. Posiada welwetowe, podobne do dalii, brązowo-czerwone kwiaty o zapachu czekolady. Jest rośliną sterylną (nie wytwarza nasion). Ma zastosowanie głównie jako roślina obwódkowa do ogrodów bylinowych. Podjęto badania, których celem było określenie wpływu usunięcia wierzchołka pędu i liści oraz sposobu ułożenia eksplantatów na indukcję pędów kątowych. Eksplantaty pobrano z zespołów pędów uzyskanych w kulturach sterylnych i prowadzono na pożywce Murashige i Skooga uzupełnionej BA 1 mg·dm-3. Wyniki badań wykazały, że usunięcie wierzchołka wzrostu z ulistnionych fragmentów pędów stymulowało ich rozkrzewianie. Usunięcie liści z przyciętych wierzchołkowych fragmentów pędów powodowało zahamowanie wytwarzania pędów kątowych. Największą liczbę pędów kątowych charakteryzujących się największą świeżą masą uzyskano z pozbawionych liści wierzchołków pędów ułożonych w pozycji odwrotnej do naturalnej. Przycięte i pozbawione liści wierzchołkowe fragmenty pędów ułożone w pozycji naturalnej wytwarzały pędy charakteryzujące się najsilniejszym wzrostem elongacyjnym i najwięcej pędów osiągało długość powyżej 1 cm.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2013
• Tom: 12
• Numer: 1
• Opis fizyczny: p.41-53,fig.,ref.

Twórcy

autor

Kozak D.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, ul.Leszczynskiego 58, 20-068 Lublin, Poland

autor

Pogroszewska E.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, ul.Leszczynskiego 58, 20-068 Lublin, Poland

autor

Szmagara M.

• Institute of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, ul.Leszczynskiego 58, 20-068 Lublin, Poland

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