Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips

• Autorzy: Kulus D. , Serocka M. , Mikula A.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.

Słowa kluczowe

EN

plant cultivation; mother chrysanthemum; Dendranthema grandiflora; Lady Orange cultivar; Lady Salmon cultivar; Richmond cultivar; plant cultivar; ornamental plant; flowering plant; chimera; shoot tip; explant; cryopreservation; dehydration; osmotic dehydration; morphogenetic response; sucrose concentration; preculture treatment

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2018
• Tom: 17
• Numer: 1
• Opis fizyczny: p.139-147,fig.,ref.

Twórcy

autor

Kulus D.

• Faculty of Agriculture and Biotechnology, University of Technology and Life Science in Bydgoszcz, Bernardynska 6, 85-029 Bydgoszcz, Poland

autor

Serocka M.

• Faculty of Agriculture and Biotechnology, University of Technology and Life Science in Bydgoszcz, Bernardynska 6, 85-029 Bydgoszcz, Poland

autor

Mikula A.

• Botanical Garden - Centre for Biological Diversity Conservation in Powsin, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland

Bibliografia

• Agbidinoukoun, A., Ahanhanzo, C., Adoukonou-Sagbadja, H., Adjassa, M., Djikpo-Tchibozo, M.A., Agbangla, C. (2013). Impact of osmotic dehydration on the encapsulated apices survival of two yams (Dioscorea spp.) genotypes from Benin. J. Appl. Biosci., 65, 4999–5007.
• Ai, P.F., Lu, L.P., Song, J.J. (2012). Cryopreservation of in vitro-grown shoot-tips of Rabdosia rubescens by encapsulation-dehydration and evaluation of their genetic stability. Plant Cell Tiss. Org. Cult., 108(3), 381–387.
• Fukai, S., Oe, M. (1990). Morphological observations of chrysanthemum shoot tips cultured after cryoprotection and freezing. J. Jpn. Soc. Hortic. Sci., 59(2), 383–387.
• Giladi, I., Altman, A., Goren, R. (1977). Differential effects of sucrose, abscisic acid and benzyladenine on shoot growth and callus formation in the abscission zone of excised Citrus buds. Plant Physiol., 59(6), 1161–1164.
• Halmagyi, A., Fischer-Kluver, G., Mix-Wagner, G., Schumacher, H.M. (2004). Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. Plant Cell Rep., 22(6), 371–375.
• Hitmi, A., Coudret, A., Barthomeuf, C., Sallanon, H. (2000). Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures. J. Plant Physiol., 157(1), 47–53.
• Jeon, M.S., Naing, A.H., Park, K.I., Kim, C.K. (2015a). The effect of Agbidinoukoun, A., Ahanhanzo, C., Adoukonou-Sagbadja, H., Adjassa, M., Djikpo-Tchibozo, M.A., Agbangla, C. (2013). Impact of osmotic dehydration on the encapsulated apices survival of two yams (Dioscorea spp.) genotypes from Benin. J. Appl. Biosci., 65, 4999–5007.
• Ai, P.F., Lu, L.P., Song, J.J. (2012). Cryopreservation of in vitro-grown shoot-tips of Rabdosia rubescens by encapsulation-dehydration and evaluation of their genetic stability. Plant Cell Tiss. Org. Cult., 108(3), 381–387.
• Fukai, S., Oe, M. (1990). Morphological observations of chrysanthemum shoot tips cultured after cryoprotection and freezing. J. Jpn. Soc. Hortic. Sci., 59(2), 383–387.
• Giladi, I., Altman, A., Goren, R. (1977). Differential effects of sucrose, abscisic acid and benzyladenine on shoot growth and callus formation in the abscission zone of excised Citrus buds. Plant Physiol., 59(6), 1161–1164.
• Halmagyi, A., Fischer-Kluver, G., Mix-Wagner, G., Schumacher, H.M. (2004). Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. Plant Cell Rep., 22(6), 371–375.
• Hitmi, A., Coudret, A., Barthomeuf, C., Sallanon, H. (2000). Role of intracellular water retention strength in freezing tolerance of Chrysanthemum cinerariaefolium Vis. cell cultures. J. Plant Physiol., 157(1), 47–53.
• Jeon, M.S., Naing, A.H., Park, K.I., Kim, C.K. (2015a). The effect of antifreeze protein on the cryopreservation of chrysanthemums. Plant Cell Tiss. Org. Cult., 123(3), 665–671.
• Jeon, S.M., Arun, M., Lee, S.Y., Kim, C.K. (2015b). Application of encapsulation-vitrification in combination with air dehydration enhances cryotolerance of Chrysanthemum morifolium shoot tips. Sci. Hortic., 194, 91–99.
• Jevremović, S., Trifunović, M., Nikolić, M., Subotić, A., Radojević, L. (2006). Clonal fidelity of chrysanthemum regenerated from long term cultures. Genetika, 38, 243–249.
• Karimi, M.Z., Amin, M.N., Azad, M.A.K., Begum, F., Islam, M.M., Alam, R. (2002). Effect of different plant growth regulators on in vitro shoot multiplication of Chrysanthemum morifolium. OnLine J. Biol. Sci., 3(6), 553–560.
• Kulus, D., Zalewska, M. (2014a). Cryopreservation as a tool used in long-term storage of ornamental species – a review. Sci. Hortic., 168, 88–107.
• Kulus, D., Zalewska, M. (2014b). In vitro plant recovery from alginate encapsulated Chrysanthemum × grandiflorum /Ramat./Kitam. shoot tips. Propag. Ornam. Plants, 14(1), 3–12.
• Lee, Y.G., Popova, E., Cui, H.Y., Kim, H.H., Park, SU, Bae, CH, Lee, S.C., Engelmann, F., 2011. Improved cryopreservation of chrysanthemum (Chrysanthemum morifolium) using droplet-vitrification. CryoLetters, 32(6), 486–497.
• Martín, C., González-Benito, E. (2009). Which role plays osmotic stress on (epi-genetic) instability. CryoLetters, 30(5), 396–397.
• Mikuła, A., Tykarska, T., Kuraś, M. (2005). Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters, 26(6), 367–378.
• Moges, A.D., Shibli, R.A., Karam, N.S. (2004). Cryopreservation of african violet (Saintpaulia ionantha Wendl.) shoot tips. In Vitro Cell Dev. – Plant, 40(4), 389–395.
• Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant., 15, 473–497.
• Nilanthi, D., Yang, Y.S. (2014). Effects of sucrose and other additives on in vitro growth and development of purple poneflower (Echinacea purpurea L.). Adv. Biol., 402309, 1–4.
• Osorio-Saenz, A., Mascorro-Gallardo, J.O., Rodríguez de la, O.J.L., López, C.M., González-Arnao, M.T. (2011). Cryopreservation of chrysanthemum shoot-tips (Dendranthema grandiflorum Kitam) by encapsulationdehydration and vitrification. Rev. Chapingo Ser Hortic., 17, 33–43.
• Reed, B.M., Uchendu, E. (2008). Controlled rate cooling. Plant cryopreservation: a practical guide. Springer, New York, 77–92.
• Roycowicz, P., Malay, J.E. (2012). Dissecting the effects of nitrate, sucrose and osmotic potential on Arabidopsis root and shoot system growth in laboratory assays. Phylosoph. Transact. Roy. Soc. B, 367(1595), 1489–1500.
• Sakai, A., Matsumoto, T., Hirai, D., Niino, T. (2000). Newly development encapsulation-dehydration protocol for plant cryopreservation. CryoLetters, 21(1), 53–62.
• Shatnawi, M.A., Johnson, K.A. (2004). Cryopreservation by encapsulation-dehydration of ‘Christams bush’ (Ceratopetalum gummiferum) shoot tips. In Vitro Cell. Dev. – Plant, 40(2), 239–244.
• Teixeira da Silva, J., Shinoyama, H., Aida, R., Matsuhita, J., Raj, S.K., Chen, F. (2013). Chrysanthemum biotechnology: Quo vadis? Cr. Rev. Plant Sci., 32(1), 21–52.
• Verleysen, H., Van Bockstaele, E., Debergh, P. (2005). An encapsulation-dehydration protocol for cryopreservation of the azalea cultivar ‘Nordlicht’ (Rhododendronsimsii Planch.). Sci. Hortic., 106(3), 402–414.
• Wang, R.R., Gao, X.X., Chen, L., Huo, L.Q., Li, M.F., Wang, Q.C. (2014). Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification. Sci. Hortic., 176, 330–339.
• Yang, D.H., Yun, P.Y., Park, S.Y., Plaha, P., Lee, D.S., Lee, I.S., Hwang, Y.S., Kim, Y.A., Lee, J.S., Han, B.H., Lee, S.Y., Suh, E.J., Lim, Y.P. (2005). Cloning, characterization and expression of a lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura). Plant Physiol. Biochem., 43(12), 1044–1051.
• Yaseen, M., Ahmad, T., Sablok, G., Standardi, A., Hafiz, I.A. (2013). Review: role of carbon sources for in vitro plant growth and development. Mol. Biol. Rep., 40(4), 2837–2849.
• Zalewska, M., Kulus, D. (2014). Improvement of Chrysanthemum × grandiflorum /Ramat./Kitam. encapsulationdehydration cryopreservation protocol. Acta Sci. Pol. Hortorum Cultus, 13(2), 97–108.
• Zalewska, M., Lema-Rumińska, J., Miler, N. (2007). In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum. Sci. Hortic., 113, 70–73.
• Zalewska, M., Tymoszuk, A., Miler, N. (2011). New chrysanthemum cultivars as a result of in vitro mutagenesis with the application of different explant types. Acta Sci. Pol. Hortorum Cultus, 10(2), 109–123. antifreeze protein on the cryopreservation of chrysanthemums. Plant Cell Tiss. Org. Cult., 123(3), 665–671.
• Jeon, S.M., Arun, M., Lee, S.Y., Kim, C.K. (2015b). Application of encapsulation-vitrification in combination with air dehydration enhances cryotolerance of Chrysanthemum morifolium shoot tips. Sci. Hortic., 194, 91–99.
• Jevremović, S., Trifunović, M., Nikolić, M., Subotić, A., Radojević, L. (2006). Clonal fidelity of chrysanthemum regenerated from long term cultures. Genetika, 38, 243–249.
• Karimi, M.Z., Amin, M.N., Azad, M.A.K., Begum, F., Islam, M.M., Alam, R. (2002). Effect of different plant growth regulators on in vitro shoot multiplication of Chrysanthemum morifolium. OnLine J. Biol. Sci., 3(6), 553–560.
• Kulus, D., Zalewska, M. (2014a). Cryopreservation as a tool used in long-term storage of ornamental species – a review. Sci. Hortic., 168, 88–107.
• Kulus, D., Zalewska, M. (2014b). In vitro plant recovery from alginate encapsulated Chrysanthemum × grandiflorum /Ramat./Kitam. shoot tips. Propag. Ornam. Plants, 14(1), 3–12.
• Lee, Y.G., Popova, E., Cui, H.Y., Kim, H.H., Park, SU, Bae, CH, Lee, S.C., Engelmann, F., 2011. Improved cryopreservation of chrysanthemum (Chrysanthemum morifolium) using droplet-vitrification. CryoLetters, 32(6), 486–497.
• Martín, C., González-Benito, E. (2009). Which role plays osmotic stress on (epi-genetic) instability. CryoLetters, 30(5), 396–397.
• Mikuła, A., Tykarska, T., Kuraś, M. (2005). Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters, 26(6), 367–378.
• Moges, A.D., Shibli, R.A., Karam, N.S. (2004). Cryopreservation of african violet (Saintpaulia ionantha Wendl.) shoot tips. In Vitro Cell Dev. – Plant, 40(4), 389–395.
• Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant., 15, 473–497.
• Nilanthi, D., Yang, Y.S. (2014). Effects of sucrose and other additives on in vitro growth and development of purple poneflower (Echinacea purpurea L.). Adv. Biol., 402309, 1–4.
• Osorio-Saenz, A., Mascorro-Gallardo, J.O., Rodríguez de la, O.J.L., López, C.M., González-Arnao, M.T. (2011). Cryopreservation of chrysanthemum shoot-tips (Dendranthema grandiflorum Kitam) by encapsulationdehydration and vitrification. Rev. Chapingo Ser Hortic., 17, 33–43.
• Reed, B.M., Uchendu, E. (2008). Controlled rate cooling. Plant cryopreservation: a practical guide. Springer, New York, 77–92.
• Roycowicz, P., Malay, J.E. (2012). Dissecting the effects of nitrate, sucrose and osmotic potential on Arabidopsis root and shoot system growth in laboratory assays. Phylosoph. Transact. Roy. Soc. B, 367(1595), 1489–1500.
• Sakai, A., Matsumoto, T., Hirai, D., Niino, T. (2000). Newly development encapsulation-dehydration protocol for plant cryopreservation. CryoLetters, 21(1), 53–62.
• Shatnawi, M.A., Johnson, K.A. (2004). Cryopreservation by encapsulation-dehydration of ‘Christams bush’ (Ceratopetalum gummiferum) shoot tips. In Vitro Cell. Dev. – Plant, 40(2), 239–244.
• Teixeira da Silva, J., Shinoyama, H., Aida, R., Matsuhita, J., Raj, S.K., Chen, F. (2013). Chrysanthemum biotechnology: Quo vadis? Cr. Rev. Plant Sci., 32(1), 21–52.
• Verleysen, H., Van Bockstaele, E., Debergh, P. (2005). An encapsulation-dehydration protocol for cryopreservation of the azalea cultivar ‘Nordlicht’ (Rhododendronsimsii Planch.). Sci. Hortic., 106(3), 402–414.
• Wang, R.R., Gao, X.X., Chen, L., Huo, L.Q., Li, M.F., Wang, Q.C. (2014). Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification. Sci. Hortic., 176, 330–339.
• Yang, D.H., Yun, P.Y., Park, S.Y., Plaha, P., Lee, D.S., Lee, I.S., Hwang, Y.S., Kim, Y.A., Lee, J.S., Han, B.H., Lee, S.Y., Suh, E.J., Lim, Y.P. (2005). Cloning, characterization and expression of a lateral suppressor-like gene from chrysanthemum (Dendranthema grandiflorum Kitamura). Plant Physiol. Biochem., 43(12), 1044–1051.
• Yaseen, M., Ahmad, T., Sablok, G., Standardi, A., Hafiz, I.A. (2013). Review: role of carbon sources for in vitro plant growth and development. Mol. Biol. Rep., 40(4), 2837–2849.
• Zalewska, M., Kulus, D. (2014). Improvement of Chrysanthemum × grandiflorum /Ramat./Kitam. encapsulationdehydration cryopreservation protocol. Acta Sci. Pol. Hortorum Cultus, 13(2), 97–108.
• Zalewska, M., Lema-Rumińska, J., Miler, N. (2007). In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum. Sci. Hortic., 113, 70–73.
• Zalewska, M., Tymoszuk, A., Miler, N. (2011). New chrysanthemum cultivars as a result of in vitro mutagenesis with the application of different explant types. Acta Sci. Pol. Hortorum Cultus, 10(2), 109–123.

Identyfikatory

DOI

10.24326/asphc.2018.1.13