The influence of the salt composition of basal medium and growth regulators on in vitro growth and development of Tibouchina urvilleana (DC.) Cogn

• Autorzy: Kozak D. , Wnuk K.

Warianty tytułu

PL

Wpływ zestawu soli mineralnych i regulatorów wzrostu na wzrost i rozwój Tibouchina urvilleana (DC.) Cogn. in vitro

• Języki publikacji: EN

Abstrakty

EN

Tibouchina urvilleana is a very popular small tree or shrub known for its beautiful velvety foliage and violet-blue flowers. It can be used in the mild climate in any landscape or as an indoor container plant in cooler regions. The aim of the present study was to determine the effect of mineral salt composition and growth regulators (BA and IAA) on the growth and development of Tibouchina urvilleana shoots. Shoot tips, obtained from aseptically grown shoots, were cultured on Murashige and Skoog (MS) (full and half strength), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA at 2.5 mg·dm⁻³ and IAA at 0.1 mg·dm⁻³. Also, the effects of BA (1.0, 2.5, 5.0 mg·dm⁻³) and IAA (0.1 or 0.5 mg·dm⁻³) applied alone or in combination were investigated. Explants cultured on medium without growth substances were used as a control. The study results showed that the application of full-strength Murashige and Skoog salt mixture has a more beneficial effect on the growth of the main shoot and the induction of axillary shoots of Tibouchina urvilleana compared to ½ MS, WPM and NN salt mixtures. The number of axillary shoots increases with the increasing concentration of BA (from 1 to 5 mg·dm⁻³), but they are characterized by a smaller length. The combined application of BA and IAA does not have a significant effect on the induction of axillary shoots. The addition of IAA at a concentration of 0.5 mg·dm⁻³ to the media containing BA (1 to 5 mg·dm⁻³) has a beneficial effect on elongation growth of axillary shoots.

PL

Tibouchina urvilleana jest bardzo popularnym niskim drzewem lub krzewem o pięknym welwetowym ulistnieniu i fioletowoniebieskich kwiatach. Może być uprawiana w gruncie w klimacie łagodnym lub w pomieszczeniu w pojemnikach w chłodnych rejonach. Celem badań było określenie wpływu zestawu soli mineralnych oraz regulatorów wzrostu (BA, IAA) na wzrost i rozwój pędów Tibouchina urvilleana. Wierzchołkowe fragmenty pędów, uzyskane z kultur aseptycznych, kultywowano na pożywce Murashige i Skooga (MS) (pełny zestaw i ½ zestawu soli), Nitsch i Nitsch (NN) lub Lloyd i McCown (WPM) zawierającej BA w stężeniu 2,5 mg·dm⁻³ i IAA w ilości 0,1 mg·dm⁻³. Zbadano również wpływ BA (1,0; 2,5; 5,0 mg·dm⁻³) i IAA (0,1 lub 0,5 mg·dm⁻³) zastosowanych pojedynczo oraz w kombinacji. Eksplantaty wyłożone na pożywkę bez regulatorów wzrostu stanowiły kontrolę. Wyniki badań wykazały, że zastosowanie pełnego zestawu soli mineralnych wg Murashige i Skooga, korzystniej wpływa na wzrost pędu głównego i indukcję pędów kątowych Tibouchina urvilleana, w porównaniu z zestawem soli ½ MS, WPM i NN. Wraz ze wzrostem stężenia BA (od 1 do 5 mg·dm⁻³) rośnie liczba pędów kątowych, lecz charakteryzują się one mniejszą długością. Łączne zastosowanie BA i IAA nie ma istotnego wpływu na indukcję pędów kątowych. Dodatek IAA w stężeniu 0,5 mg·dm⁻³ do pożywek zawierających BA (1–5 mg·dm⁻³) wpływa korzystnie na wzrost wydłużeniowy pędów kątowych.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 6
• Opis fizyczny: p.59-68,fig.,ref.

Twórcy

autor

Kozak D.

• Department of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, Leszczynskiego 58, 20-068 Lublin, Poland

autor

Wnuk K.

• Department of Ornamental Plants and Landscape Architecture, University of Life Sciences in Lublin, Leszczynskiego 58, 20-068 Lublin, Poland

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