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2019 | 26 |

Tytuł artykułu

The effectivity of metabolite secondary extract of bacteria associated with sea grass (Cymodocea rotundata) for Vibriosis treatment in tiger shrimp (Penaeus monodon)

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The natural occurrence of the shrimp (Penaeus monodon Fabricius, 1798) and seagrass (Cymodocea rotundata Asch. & Schweinf.) is found in the area of Indian Ocean, Indonesia, for central Pacific. Vibriosis can cause death in larvae, juvenile and adult shrimp almost close to 100%. Antibacterial substances from secondary metabolites are produced by plants and animals to reduce the use of synthetic antibiotics. The purpose of this research was to determine the ability of secondary metabolites contained in the bacterial extract of the association of sea grass Cymodocea rotundata (isolate code BA.1) to treat vibriosis in tiger shrimp (Penaeus monodon). This research was conducted at the Laboratory of Integrated Biotechnology and Laboratory of Aquaculture, Fisheries and Marine Sciences Faculty, Universitas Padjadajaran, and at the Brackish and Southern Ocean Aquaculture Development Center Pangandaran, from June to September 2018. The research method used was experimental, with completely randomized design (CRD) consisting of five treatments with three replications including; control treatment (concentration of 0 mg·L–1), 150 mg.L–1, 300 mg·L–1 , 450 mg·L–1 and 600 mg·L–1. Observations made include clinical symptoms (morphology, behavior) and survival rate. Clinical symptoms of infected tiger shrimp during the in vivo test were red spots on the abdomen and necrosis of some leg and tail segments. The behavior of tiger shrimp mostly shows less responsive movements to fish-feed on the first day, and showing responsive and active behavior on the fifth day. In vivo test results for 14 days showed that the highest survival rate of 51.67% was in case of the addition of antibacterial extracts of BA.1 with a concentration of 300 mg·L–1.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

26

Opis fizyczny

p.96-105,fig.,ref.

Twórcy

autor
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
autor
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
autor
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia
autor
  • Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Sumedang, Indonesia

Bibliografia

  • [1] F. Khamesipour, E. Noshadi, M. Moradi and M. Raissy. Detection of Vibrio spp. in Shrimp from Aquaculture Site in Iran Using Polimerase Chain Reaction (PCR). AACL Bioflux. Vol. 7 No. 1 (2014) 1-7.
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  • [3] J. Perez, C.C Shen, C.Y. Ragasa. Chemical Constituents of Cymodocea rotundata Asch. and Schweinf. Pharmacognosy Journal, 10(4) (20180 620-621.
  • [4] N. Artanti, F. Maryani, H. Mulyani, R. T. Dewi, V. Saraswati and T. Murniasih, Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges. Annales Bogorienses, Vol. 20 No. 1 (2016) 23-28.
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  • [6] S.V. Alavandi, V. Manoranjita, K.K. Vijayan, N. Kalaimani, T.C. Santiago. Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letters in Applied Microbiology Volume 43, Issue 5 November 2006 Pages 566-570, https://doi.org/10.1111/j.1472-765X.2006.01986.x
  • [7] W. W. Davis, T. S. Strout. Disk Plate Method of Microbiological Antibiotic Assay: I. Factor Influencing Variability and Error 1. Appl. Microbiol., 22(4) (1971) 695-665.
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  • [9] Sarjito, N.E.W. Ningrum., O.K. Radjasa, and S.B. Prayitno. Application of Repetitive Sequence-Based PCR on the Richness of Vibrio on the Tiger Shrimp (Penaeus monodon Fab.). Journal of Coastal Development, 15(3) (2012) 303-309.
  • [10] P. Montero, A. Avalos, and M. Perez Mateos. Characterization of polyphenoloxidase of prawns (Penaeus japonicus). Alternatives to inhibition: additives and high pressure treatment. Food Chem., 75 (2001) 317-324.
  • [11] S. Y. Hung, D. G. Boucias. Phenoloksidase Activity in Hemolymph of Naïve and Bauveria bassiana-Infected Spodoptera Exigua Larvae. Academic Press, Inc., Florida (1996).
  • [12] G. Aguirre-Guzman, H. M. Ruiz and F. Ascencio. A review of extracellular virulence product of Vibrio species important in diseases of cultivated shrimp. Aquac. Res., 35 (2004) 1395-1404.
  • [13] K.M. Spann, J.E. Vickers, & R.J.G Lester. Lymphoid organ virus of Penaeus monodon from Australia. Diseases of Aquatic Organisms, 23 (1995) 127-134.
  • [14] C. Chantanachookin, S. Boonyaratpalin, J. Kasornchandra, S. Direkbusarakom, U. Ekpanithanpong, K. Supamataya, S. Siurairatana, & T.W. Flegel. Histology and ultrastructure reveal a new granulosis-like virus in Penaeus monodon affected by ‘yellow-head’ disease. Diseases of Aquatic Organisms, 17 (1993) 145-157.
  • [15] Md. A. Aziz, A.S. Alam, A.A. Ema, M. Akter. Analysis of Secondary Metabolites, Antibacterial, Brine Shrimp Lethality & Larvicidal Potentiality of Microcos paniculata Fruits. IOSR Journal of Pharmacy and Biological Sciences, Volume 9, Issue 3 Ver. V (May -June 2014), PP 50-58.
  • [16] F.E. Raquel. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochimica et Biophysica Acta (BBA) - Biomembranes, Volume 1768, Issue 10, October 2007, pp. 2500-2509.
  • [17] S. Boonyaratpalin, K. Supamataya, J. Kasornchandra, S. Direkbusarakom, U. Aekpanithanpong, & C. Chantanachookhin. Non-occluded baculo-like virus the causative agent of yellow-head disease in the black tiger shrimp Penaeus monodon. Fish Pathology, 28 (1993) 103-109.
  • [18] P.S. Chang, C.-F. Lo, G.-H. Kou, C.C. Lu, & S.N. Chen. Purification and amplification of DNA from Penaeus monodon-type baculovirus (MBV). Journal of Invertebrate Pathology, 62 (1993) 116-120

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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