In vitro replication of recombinant Marek’S disease viruses constructed from field strains lacking meq and vtr oncogenes

• Autorzy: Wozniakowski G. , Samorek-Salamonowicz E.
• Języki publikacji: EN

Abstrakty

EN

The study describes construction of five recombinant very virulent (vv) and very virulent plus (vv+) strains lacking meq and viral telomerase (vTR). Deletion of both copies of meq and vTR was achieved by Red E/T recombination in GS1783 E. coli cells. The constructed bacterial artificial chromosome (BAC) clones reconstituted in chicken embryo fibroblasts were examined by immunofluorescence assay to compare the features of recombinant strains with wild-type viruses. The results demonstrated that recombinant BAC strains caused slightly reduced cytophatic effect and decreased level of the fluorescence obtained from the monoclonal antibody in comparison to the parental viruses. Generation of recombinant BAC clones may provide more detailed information on the function of Marek's disease virus oncogenes and the potential use of recombinants for the preparation of the new vaccine against Marek's disease.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 2
• Opis fizyczny: p.141-147,fig.,ref.

Twórcy

autor

Wozniakowski G.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Samorek-Salamonowicz E.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Baigent S.J., Petherbridge L.J., Smith L P., Zhao Y., Chesters P.M., Nair V.K.: Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek's disease. J Gen Virol 2006, 87, 769-776.
• 2. Becker Y., Asher Y., Tabor E., Davidson I., Malkinson M., Weisman Y.: Polymerase chain reaction for differentiation between pathogenic and non-pathogenic serotype 1 Marek's disease viruses (MDV) and vaccine viruses of MDV-serotypes 2 and 3. J Virol Methods 1992, 40, 307-322.
• 3. Brown A.C., Smith L.P., Kgosana L., Baigent S.J., Nair V., Allday M.J.: Homodimerization of the Meq viral oncoprotein is necessary for induction of T-cell lymphoma by Marek's disease virus. J Virol 2009, 83, 11142-11151.
• 4. Greider C.W., Blackburn E.H.: The telomere terminal transferase of tetrahymena is a ribonucleoprotein enzyme with 2 kinds of primer specificity. Cell 1987, 51, 887- 898.
• 5. Jarosiński K.W., Arndt S., Kaufer B.B., Osterrieder N.: Fluorescently tagged pUL47 of Marek's disease virus reveals differential tissue expression of the tegument protein in vivo. J Virol 2012, 86, 2428-2436.
• 6. Jarosinski K.W., Osterrieder N.: Marek's disease virus expresses multiple UL44 (gC) variants through mRNA splicing that are all required for efficient horizontal transmission. J Virol 2012, 86, 7896-7906.
• 7. Kaufer B.B., Arndt S., Trapp S., Osterrieder N., Jarosinski K.W.: Herpesvirus telomerase RNA (vTR) with a mutated template sequence abrogates herpesvirus- induced lymphomagenesis. PLoS Pathog 2011, 7, doi: 10.1371/journal.ppat. 1002333.
• 8. Kim T, Mays J., Fadly A., Silva R.F.: Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek's disease virus (MDV) alters expression of nearby MDV genes. Virus Genes 2011, 42, 369-376.
• 9. Messerle M., Crnkovic I., Hammerschmidt W., Ziegler H., Koszinowski U.H.: Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proc Nat Acad Sci USA 1997, 94, 14759- 14763.
• 10. Mays J.K., Silva R.F., Kim T., Fadly A.: Insertion of reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of a very virulent Marek's disease virus alters its pathogenicity. Avian Pathol 2012, 41, 259-265.
• 11. Nair V.: Evolution of Marek's disease - a paradigm for incessant race between the pathogen and the host. Vet J 2005, 170, 175-183.
• 12. Petherbridge L., Howes K., Baigent S.J., Sacco M.A., Evans S., Osterrieder N., Nair V.K.: Replication- competent bacterial artificial chromosomes of Marek's disease virus: novel tools for generation of molecularly defined herpesvirus vaccines. J Virol 2003, 77, 8712- 8718.
• 13. Petherbridge L., Brown A.C., Baigent S.J., Howes K., Sacco M.A., Osterrieder N., Nair V.K.: Oncogenicity of virulent Marek's disease virus cloned as bacterial artificial chromosomes. J Virol 2004, 78, 13376-13380.
• 14. Schumacher D., Tischer B.K., Fuchs W., Osterrieder N.: Reconstitution of Marek's disease virus serotype 1 (MDV-1) from DNA cloned as a bacterial artificial chromosome and characterization of a glycoprotein B- negative MDV-1 mutant. J Virol 2000, 74, 11088-11098.
• 15. Singh S.M., Baigent S.J., Petherbridge L.J., Smith L.P., Nair V.K.: Comparative efficacy of BAC-derived recombinant SB-1 vaccine and the parent wild type strain in preventing replication, shedding and disease induced by virulent Marek's disease virus. Res Vet Sci 2010, 89, 140-145.
• 16. Silva R.F., Dunn J.R., Cheng H.H., Niikura M.: A MEQ- deleted Marek's disease virus cloned as a bacterial artificial chromosome is a highly efficacious vaccine. Avian Dis 2010, 54, 862-869.
• 17. Sun A., Petherbridge L., Zhao Y., Li Y., Nair V.K., Cui Z.: A BAC clone of MDV strain GX0101 with REV- LTR integration retained its pathogenicity. Chin Sci Bull 2010, 54, 2641-2647.
• 18. Sun A., Xu X., Petherbridge L., Zhao Y., Nair V., Cui Z.: Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek's disease virus. Virology 2010, 397, 270-276.
• 19. Tischer B.K., Schumacher D., Beer M., Beyer J., Teifke J.P., Osterrieder K., Wink K., Zelnik V., Fehler F., Osterrieder N.: A DNA vaccine containing an infectious Marek's disease virus genome can confer protection against tumorigenic Marek's disease in chickens. J Gen Virol 2002, 83, 2367-2376.
• 20. Trapp S., Parcells M.S., Kamil J.P., Schumacher D., Tischer B.K., Kumar P.M., Nair V.K, Osterrieder N.: A virus-encoded telomerase RNA promotes malignant T cell lymphomagenesis. J Exp Med 2006, 203, 1307- 1317.
• 21. Witter R.L.: Increased virulence of Marek's disease virus field isolates. Avian Dis 1997, 41, 149-163.