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2014 | 23 | 5 |

Tytuł artykułu

PCB biodegradation and bphA1 gene expression induced by salicylic acid and biphenyl with pseudomonas fluorescence P2W and ralstonia eutropha H850

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Organic carbon substrate amendments are promising bioremediation strategies to induce polychlorinated biphenyls (PCB) aerobic degradation. However, their selective induction on PCB degraders has not been well studied. In this study, the substrate interaction effects of salicylic acid and biphenyl on PCB biodegradation were investigated with pure cultured isolates, including a newly isolated Pseudomonas fluorescence (P2W) and the veteran PCB degrader Ralstonia eutropha (H850). A significant biodegradation of lower-chlorinated PCB in H850 was induced by both salicylic acid and biphenyl amendments, while the biodegradation in P2W was induced only by salicylic acid. The binary substrates of salicylic acid and biphenyl resulted in a significantly inhibited effect on PCB removal in both strains. The expression of the functional gene bphA1 in the upper biphenyl degradation pathway was further investigated by quantitative reverse transcription PCR. Compared to H850, P2W had higher expression in the bphA1 gene induced mainly by salicylic acid rather than biphenyl. Particularly, the binary substrate induction led to an excessive expression of bphA1 gene in both strains, which was in good agreement with their biomass growth. These results suggested that the special induction of PCB biodegradation depends on the selection of organic carbon substrates and the acclimation of degrader strains.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

23

Numer

5

Opis fizyczny

p.1591-1598,fig.,ref.

Twórcy

autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
autor
  • Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

Bibliografia

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  • 2. GRABOWSKA I. Polychlorinated biphenyls (PCBs) in Poland: occurrence, determination and degradation. Pol. J. Environ. Stud. 19, 7, 2010.
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  • 5. JIA L.Y., ZHENG A.P., XU L., HUANG X.D., ZHANG Q., YANG F.L. Isolation and characterization of comprehensive biphenyl-degrading bacterium, Enterobacter sp LY402. J. Microbiol. Biotechnol. 18, 952, 2008.
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  • 12. LUO W.S., D'ANGELO E.M., COYNE M.S. Plant sec­ondary metabolites, biphenyl, and hydroxypropyl P- cyclodextrin effects on aerobic polychlorinated biphenyl removal and microbial community structure in soils. Soil Biol. Biochem. 39, 735, 2007.
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  • 20. PARK Y.I., SO J.S., KOH S.C. Induction by carvone of the polychlorinated biphenyl (PCB)-degradative pathway in Alcaligenes eutrophus H850 and its molecular monitoring. J. Microbiol. Biotechnol. 9, 804, 1999.
  • 21. ZHANG Y.C., HU C.H., LUO W.S. Influences of electron donor, bicarbonate and sulfate on bioreduction processes and manganese/copper redistributions among minerals in a water- saturated Sediment. Soil Sediment Contamin. 23, 94, 2014.
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  • 23. WANG Y.P., ZHU K., ZHENG Y.M., WANG H.T., DONG G.W., HE N., LI Q.B. The effect of recycling flux on the per­formance and microbial community composition of a biofilm hydrolytic-aerobic recycling process treating anthraquinone reactive dyes. Molecules 16, 9838, 2011.
  • 24. LUO W.S., HU C.H. Interactions of plant secondary metabolites and organic carbon substrates affected on biodegradation of polychlorinated biphenyl. J. Environ. Biol., 34, 337, 2013.
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  • 26. PARNELL J.J., PARK J., DENEF V., TSOI T., HASHSHAM S., QUENSEN J., TIEDJE J.M. Coping with polychlorinated biphenyl (PCB) toxicity: physiological and genome-wide responses of Burkholderia xenovorans LB400 to PCB-mediated stresss. Appl. Environ. Microbiol., 72, 6607, 2006.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-7763d323-1545-4f3b-ab08-29fbba7e9e5e
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