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2019 | 59 | 1 |

Tytuł artykułu

Morphological, virulence and genetic variability of Ulocladium atrum causing potato leaf blight disease in Iran

Autorzy

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Potato leaf blight disease caused by Ulocladium atrum (Syn. Stemphylium atrum) is an important and epidemic disease in potato-growing regions of Iran. In this study, 30 isolates of the disease were collected from the main potato-growing regions of Iran and were analyzed on the basis of morphological characterization and pathogenicity. Based on morphological characteristics, all isolates were identified as U. atrum. Pathogenicity studies indicated that all 30 isolates were pathogenic on potato “Agria” to varying degrees. Five U. atrum isolates causing potato leaf blight disease, obtained from the Plant Pathology Laboratory, Isfahan Research Center for Agriculture and Natural Resources, Isfahan, Iran, were also examined in this study. A total of 35 isolates were genetically analyzed using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. Cluster analysis using the un-weighted pair group method with the arithmetic average (UPGMA) method for RAPD marker revealed no clear grouping of the isolates obtained from different geographical regions. The groupings, based on morphological characteristics, virulence variability and RAPD analysis, were not correlated. Cluster analysis using Jaccard’s coefficient for ISSR divided the U. atrum isolates into four main groups, in which there was no significant correlation between the isolate groupings regarding their geographic location and pathogenicity. Using molecular techniques genetic variability was detected among the accessions, with cophenetic correlation coefficients (CCC) of 0.80 for RAPDs and 0.89 for ISSRs. The RAPD and ISSR marker results corresponded well, with a correlation of 0.55.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

59

Numer

1

Opis fizyczny

p.41-49,fig.,ref.

Twórcy

  • Plant Protection Research Department, Isfahan for Agriculture and Natural Resources Research and Education Center, (AREEO) Isfahan, Iran

Bibliografia

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  • Bagherabadi S., Zafari D., Soleimani M.J. 2015. Genetic diversity of Alternaria alternata isolates causing potato brown leaf spot, using ISSR markers in Iran. Journal of Plant Pathology and Microbiology 6 (7): 1−6. DOI: https://doi:10.4172/2157-7471.1000286
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  • Noelting M.C., Sisterna M., Lovisolo M., Lori G., Sandova M.C., Sulyok M., Molina M.C. 2016. Discoloured seeds of amaranth plant infected by Alternaria alternata: physiological, histopathological alterations and fungal secondary metabolites associated or registered. Journal of Plant Protection Research 56 (3): 242−239. DOI: https://doi.org/10.1515/jppr-2016-0036.
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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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