Species-specific sensitivity of coagulase-negative staphylococci to single antibiotics and their combinations

• Autorzy: Szymanska G. , Szemraj M. , Szewczyk E. M.
• Języki publikacji: EN

Abstrakty

EN

The activity of β-lactam antibiotics (oxacillin, cloxacillin, cephalotin), vancomycin, gentamicin and rifampicin applied in vitro individually and in combination against 37 nosocomial methicillin-resistant strains of coagulase-negative staphylococci (CNS) was assessed to demonstrate the heterogeneity of this group of bacteria and estimate the chance of the efficacy of such therapy. The strains belonged to four species: Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus cohnii, Staphylococcus hominis. They originated from a hospital environment and from the skin of medical staff of the intensive care unit of a paediatric ward at a university hospital. All strains were methicillin-resistant, according to CLSI standards, but individual strains differed in MICOX values. Susceptibility to other tested antibiotics was also characteristic for the species. The increased susceptibility to antibiotics in combinations, tested by calculating the fractional inhibitory concentration (FIC) index, concerned 26 out of 37 investigated strains and it was a feature of a particular species. Combinations of vancomycin and cephalotin against S. epidermidis and oxacillin with vancomycin were significant, as well as cephalotin and rifampicin in growth inhibition of multiresistant S. haemolyticus strains.

Słowa kluczowe

EN

Staphylococcus; antibiotic synergism; methicillin resistance; coagulase; antibiotic; combination; infection; treatment

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 2
• Opis fizyczny: p.155-161,fig.,ref.

Twórcy

autor

Szymanska G.

• Department of Biosynthesis of Drugs, Medical University of Lodz, Lodz, Poland

autor

Szemraj M.

autor

Szewczyk E. M.

Bibliografia

• Bourgeois I., M. Pestel-Caron, J. Lemeland, J.L. Pons and F. Caron. 2007. Tolerance to the glycopeptides vancomycin and teicoplanin in coagulase-negative staphylococci. Antimicrob. Agents Chemiother. 51: 740–743.
• Chandran A.U. and R. Rennie. 2005. Routine antimicrobial susceptibility testing of coagulase-negative staphylococci isolated from blood cultures: is it necessary? Clin. Microbiol. Infect. 11: 1037–1040.
• Climo M.W, R.L. Patron and G.L. Archer. 1999. Combinations of vancomycin and β-lactams are synergistic against staphylococci with reduced susceptibilities to vancomycin. Antimicrob. Agents Chemiother. 43: 1747–1753.
• Clinical and Laboratory Standards Institute. 2008. Performance standards for antimicrobial Susceptibility testing. Eighteenth Informational Supplement. M100-S18. CLSI, Wayne, PA, USA.
• Dawis M.A., H.D.Isenberg, K.A. France and S.G. Jenkins. 2003. In vitro activity of gatifloxacin alone and in combination with cefepime, meropenem, piperacillin and gentamicin against multidrug-resistant organism. J. Antimicrob. Chemother. 51: 1203–1211.
• Finkelstein R., R. Fusman, I. Oren, I. Kassis and N. Hashman. 2002. Clinical and epidemiologic significance of coagulase-negative staphylococci bacteremia in a tertiary care university Israeli hospital. Am. J. Infect. Control. 30: 21–5.
• Fox P.M., R.J. Lampen, K.S. Stumpf, G.L. Archer and M.W. Climo. 2006. Succesful therapy of experimental endocarditis caused by vancomycin-resistant Staphylococcus aureus with a combination of vancomycin and β-lactam antibiotics. Antimicrob. Agents Chemiother. 50: 2951–2956.
• Frigatto E.A., A.M. Machado, A.C. Pignatari and A.C. Gales. 2005. Is the cefoxitin disk test reliable enough to detect oxacillin resistance in coagulase-negative staphylococci? J. Clin. Microbiol. 43: 2028–2029.
• Guerrero M.L.F. and M. Górgolas. 2006. Comparative activity of cloxacillin and vancomycin against methicillin-susceptible Staphylococcus aureus experimental endocarditis. J. Antimicrob. Chemother. 58: 1066–1069.
• Hamels S., J.L. Gala, S. Dufour, P. Vannuffel, N. Zammatteo and J. Remacle. 2001. Consensus PCR and microarray for diagnosis of the genus Staphylococcus species, and methicillin resistance. Biotechniq. 31: 1364–6.
• John M.A., C. Pletch and Z. Hussain. 2002. In vitro activity of quinupristin/dalfopristin, linszolid, telithromycin and comparator antimicrobial agents against 13 species of coagulase-negative staphylococci. J. Antimicrob. Chemother. 50: 933–938.
• Kloos W.E. 1997. Taxonomy and systematic of staphylococci indigenous to humans. pp. 123–125. In: Crossley K.B. and G.L. Archer (eds). The Staphylococci in Human Disease. Churchill Livingstone, London.
• Kloos W.E. and T.L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol. Rev. 7: 117–140.
• Krediet T.G., M.E. Jones, L.J. Gerards and A. Fleer. 1999. Clinical outcome of cephalotin versus vancomycin therapy in the treatment of coagulase-negative staphylococcal septisemia in neonates: Relation to methicillin resistance and mec A gene carriage of blood isolates. Pediatrics 103: 3.
• Kuti J.L., C.R.V. Kiffer, C.M.F. Mendes and D.P. Nicolau. 2008. Pharmacodynamics comparsion of linezolid, teicoplanin and vancomycin against clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci collected from hospitals in Brasil. Clin. Microbiol. Infect. 14: 116–123.
• Lawrence S.L., V. Roth, R. Slinger, B. Toye, I. Gaboury and B. Lemyre. 2005. Cloxacillin versus vancomycin for presumed lateonset sepsis in the Neonatal Intensive care Unit and the impact upon outcome of coagulase negative staphylococcal bacteremia: a retrospective cohort study. BMC Pediatrics 5: 49.
• Lee D., H. Chun, D. Yim, S.M. Choi, J.H. Choi, J.H. Yoo, W.S. Shin and M.W. Kang. 2003. Efficacies of vancomycin, arbekacin, and gentamicin alone or in combination against methicillin-resistant Staphylococcus aureus in an in vitro infective endocarditis model. Antimicrob. Agents Chemiother. 47: 3768–3773.
• Martins A. and R.S. Cunha Maria de Lourdes. 2007. Methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci: Epidemiological and molecular aspects. Microbiol. Immunol. 51: 787–795.
• Miranda-Nowales G., B.E. Leanos-Miranda, M. Vilchis-Perez and F. Solórzano-Santos. 2006. In vitro activity effects of combinations of cephalotin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains. Ann. Clin. Microb. Antimicrob. 5: 25.
• Nowak T, E. Balcerczak, M. Mirowski and E.M. Szewczyk. 2006. Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci. Pol. J. Microbiol. 55: 339–343.
• Szewczyk E.M., A. Piotrowski and M. Różalska. 2000. Predominant staphylococci in the intensive care unit of a pediatric hospital. J. Hosp. Infec. 45: 145–154.
• Szewczyk E.M., M. Różalska, T. Cieślikowski and T. Nowak. 2004. Plasmids of Staphylococcus cohnii isolated from the intensive-care unit. Folia Microbiol. 49: 123–131.
• Tacconelli E., E.M.C. D’Agata and A.W. Karchmer. 2003. Epidemiological comparison of true methicillin-resistant and methicillinsusceptible coagulase-negative staphylococcal bacteremia at hospital admission. Clin. Infect. Dis. 37: 644–649.
• Tan T.Y., S.Y. Ng, W.X. Ng. 2006. Clinical significance of coagulase-negative Staphylococci recovered from nonsterile sites. J. Clin. Microbiol. 44: 3413–3414.
• Van Griethuysen A., M. Pouw, van Leewen M. Heck, P. Willemse, A. Buiting, and J. Kluytmans. 1999. Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 2789–2792.
• Walsh T.R., A. Bolmström, A. Qwärnström, P. Ho, M. Wootton, R.A. Howe, A.P. MacGowan, and D. Diekema. 2001. Evaluation of current methods for detection of Staphylococci with reduced susceptibility to glycopeptides. J. Clin. Microbiol. 39: 2439–2444.
• Wang Y.C. and M. Lipstich. 2006. Upgrading antibiotic use within a class: Tradeoff between resistance and treatment success. PNAS. 103: 9655–9660.
• Weinstein M.P., S. Mirrett, L. van Pelt, M. Mc-Kinnon, B.L. Zimmer, W. Kloos and L.B. Reller. 1998. Clinical importance of identifying coagulase-negative staphylococci isolated from blood cultures: Evaluation of microscan rapid and dried overnight gram-positive panels versus a conventional reference method. J. Clin. Microbiol. 36: 2089–2092.