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2014 | 23 | 5 |

Tytuł artykułu

Influence of aromatic, heteroaromatic, and alkane hydrocarbons on the lipase activity of Pseudomonas sp. in batch culture

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The induction of the lipolytic system through the use of some hydrocarbons as the only carbon source for the growth of Pseudomonas sp. was investigated. The results showed induction of the enzymatic activity using 4 of 11 hydrocarbons used at a concentration of 3,000 mg/L (butylbenzene, 1,2,3,4-tetrahydronaphthalene, hexane and benzene), which suggests that the enzymatic expression is a function of the hydrocarbon used. Furthermore, when the concentration of these hydrocarbons was increased to 5,000 mg/L the enzymatic activity was not detected. Additionally, the effect of an external source of energy, such as sucrose, and the effect of surfactant Triton X-100 were determined. The results revealed that the presence of these may benefit the induction of the enzyme or may affect it negatively and suppress it depending of the hydrocarbon being used. Therefore, it is not feasible to consider the enzymatic activity of lipase as a biomarker in the degradation of all petroleum hydrocarbons.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

23

Numer

5

Opis fizyczny

p.1507-1513,fig.,ref.

Twórcy

  • Department of Chemistry, Academic Coordination Altiplano Region – Autonomous University of San Luis Potosi, Road Cedral km. 5600 CP 78700, Matehuala, San Luis Potosi, Mexico
  • Department of Chemistry, Academic Coordination Altiplano Region – Autonomous University of San Luis Potosi, Road Cedral km. 5600 CP 78700, Matehuala, San Luis Potosi, Mexico
  • Department of Chemistry, Academic Coordination Altiplano Region – Autonomous University of San Luis Potosi, Road Cedral km. 5600 CP 78700, Matehuala, San Luis Potosi, Mexico
  • Faculty of Engineering - Institute of Metallurgy, Autonomous University of San Luis Potosi, Av. 550 Lomas Sierra Leone the 2nd Sec. CP 78210 San Luis Potosi, Mexico
  • National School of Biological Sciences – National Polytechnic Institute, Extending Carpio and Plan de Ayala s/n, Col. Santo Tomas Miguel Hidalgo CP 11340 Mexico, DF

Bibliografia

  • 1. MALIA M. P., CLOETE T.E. The use of biological activi­ties to monitor the removal of fuel contaminants-perspective for monitoring hydrocarbon contamination: a review. Int. Biodeter. Biodegr. 55, 1, 2005.
  • 2. MARGESIN R., ZIMMERBAUER A., SCHINNER F. Soil lipase activity - a useful indicator of oil biodegradation. Biotechnol. Tech. 13, 859, 1999.
  • 3. MARGESIN R., HAMMERLE M., TSCHERKO D. Microbial activity and community composition during bioremediation of diesel-oil-contaminated soil: effects of hydrocarbon concentration, fertilizers and incubation time. Microbial. Ecol. 53, 259, 2007.
  • 4. KIREEVA N. A., TARASENKO E. M., SHAMAEVA A. A., NOVOSELOVA E. I. Effect of oil and oil products on lipase activity in gray forest soil. Eurasian Soil Sci. 39, 905, 2006.
  • 5. CERVANTES-GONZÁLEZ E., SALAZAR-QUINTANIL- LA L. M., DÍAZ-FLORES P.E. Lipases induced by petrole­um hydrocarbons. Revista Internacional de Contaminación Ambiental 29, (2), 9, 2013.
  • 6. CERVANTES-GONZÁLEZ E., ROJAS-AVELIZAPA N. G., CRUZ-CARAMILLO R., GARCÍA-MENA J., ROJAS- AVELIZAPA L. I. Oil-removal enhancement in media with keratinous or chitinous wastes by hydrocarbon-degrading bacteria isolated from oil-polluted soils. Environ. Technol. 29, 171, 2008.
  • 7. CHILLCOTT R. P. Compendium of Chemical Hazards: Kerosene (Fuel Oil). Health Protection Agency (HPA). Chemical Hazards and Poisons Division (CHAPD HQ). Chilton, Didcot, Oxfordshire, OX11 0RQ, United Kingdom. 2006.
  • 8. MARGESIN R., FELLER G., HAMMERLE M., STEGNER U., SCHINNER F. A colorimetric method for the determina­tion of lipase activity in soil. Biotechnol. Lett. 24, 27, 2002.
  • 9. LIN S. F., CHIOU C. M., TSAI Y. C. Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoal- caligenes F-111. Biotechnol. Lett. 17, 959, 1995.
  • 10. BOEKEMA B. H. L., BESELIN A., BREUER M., HAUER B., KOSTER M., ROSENAU F., JAEGER K. E., TOM- MANSSEN J. Hexadecane and tween 80 stimulate lipase production in Burkholderia glumae by different mecha­nisms. Appl. Environ. Microb. 73, 3838, 2007.
  • 11. PLOU F.J., FERRER M., NUERO O. M., CALVO M. V., ALCALDE M., REYES F., BALLESTEROS A. Analysis of Tween 80 as an esterase/ lipase substrate for lipolytic activ­ity assay. Biotechnol. Tech. 12, 183, 1998.
  • 12. JING-LIANG, BING-HUNG. Surfactant-mediated biodegradation of polycyclic aromatic hydrocarbons. Materials. 2, (1), 76, 2009.
  • 13. PRENAFETA-BOLDU F. X., BALLERSTEDT H., GER- RITSE J., GROTENHUIS J.T.C. Bioremediation of BTEX hydrocarbons: Effect of soil inoculation with the toluene- growing fungus Cladophialophora sp. strain T1. Biodegradation 15, 59, 2004.
  • 14. JOSEPH B., UPADHYAYA S., RAMTEKE P. Production of cold-active bacterial lipases through semisolid state fermen­tation using oil cakes. Enzyme Res. 2011, 1, 2011.
  • 15. GITI EMTIAZI-MOHAMMAH H., HABIBI-AMIR R. Production of thermostable extracellular lipase by Pseudomonas grown on cotton cake and cod removal of sunflower oil waste. Fresen. Environ. Bull. 7, 704, 2007.
  • 16. BREUIL C., SHINDLER D. B., SIJHER J. S., KUSHNER D. J. Stimulation of lipase production during bacterial growth on alkanes. J. Bacteriol. 133, 60, 1978.
  • 17. KANWAR L., GOGOI, GOSWAMI P. Production of a Pseudomonas lipase in n-alkane substrate and its isolation using an improved ammonium sulfate precipitation tech­nique. Bioresource Technol. 84, 207, 2002.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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