Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

• Autorzy: Chrobak D. , Kizerwetter-Swida M. , Rzewuska M. , Binek M.
• Języki publikacji: EN

Abstrakty

EN

A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius- specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

Słowa kluczowe

EN

antibiotic resistance; dog; Staphylococcus intermedius; practical implication; mecA gene; prevalence

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2011
• Tom: 14
• Numer: 2
• Opis fizyczny: p.213-218,ref.

Twórcy

autor

Chrobak D.

• Division of Bacteriology and Molecular Biology, Department of Pre-Clinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Kizerwetter-Swida M.

autor

Rzewuska M.

autor

Binek M.

Bibliografia

• Bannoehr J, Ben Zakour NL, Waller AS, Guardabassi L, Thoday KL, van den Broek AH, Fitzgerald JR (2007) Population genetic structure of the Staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains. J Bacteriol 189: 8685-8692.
• Bannoehr J, Franco A, Iurescia M, Battisti A, Fitzgerald JR (2009) Molecular diagnostic identification of Staphylococcus pseudintermedius. J Clin Microbiol 47: 469-471.
• Bes M, Saidi SL, Becharnia F, Meugnier H, Vandenesch F, Etienne J, Freney J (2002) Population diversity of Staphylococcus intermedius isolates from various host species: typing by 16S-23S intergenic ribosomal DNA spacer polymorphism analysis. J Clin Microbiol 40: 2275-2277.
• Carlotti DN, Guaguere E, Pin D, Jasmin P, Thomas E, Guiral V (1999) Therapy of difficult cases of canine pyoderma with marbofloxacin: a report of 39 dogs. J Small Anim Pract 40: 265-270.
• Clinical Laboratory Standards Institute (CLSI) (2008) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard – Third edition. M31-A3, Vol.28, No.8. CLSI, Wayne PA.
• Devriese LA, Hermans K, Baele M, Haesebrouck F (2008) Staphylococcus pseudintermedius versus Staphylococcus intermedius. Vet Microbiol 133: 206-207.
• Dowling PM (1996) Antimicrobial therapy of skin and ear infections. Can Vet J 37: 695-699.
• Epstein CR, Yam WC, Peiris JS, Epstein RJ (2009) Methicillin-resistant commensal staphylococci in healthy dogs as a potential zoonotic reservoir for community-acquired antibiotic resistance. Infect Genet Evol 9: 283-285.
• Finlay JE, Miller LA, Poupard JA (1997) Interpretive criteria for testing susceptibility of staphylococci to mupirocin. Antimicrob Agents Chemother 41: 1137-1139.
• Fulham KS, Lamarie SL, Hosgood G, Dick HL (2011) In vitro susceptibility testing of meticillin-resistant and meticillin-susceptible staphylococci to mupirocin and novobiocin. Vet Dermatol 22: 88-94.
• Futagawa-Saito K, Suzuki M, Ohsawa M, Ohshima S, Sakurai N, Ba-Thein W, Fukuyasu T (2004) Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons. J Appl Microbiol 96: 1361-1366.
• Gortel K, Campbell KL, Kakoma I, Whittem T, Schaeffer DJ, Weisiger RM (1999) Methicillin resistance among staphylococci isolated from dogs. Am J Vet Res 60: 1526-1530.
• Guardabassi L, Schwarz S, Lloyd DH (2004) Pet animals as reservoirs of antimicrobial-resistant bacteria. J Antimicrob Chemother 54: 321-332.
• Hájek V (1976) Staphylococcus intermedius, a new species isolated from animals. Int J Syst Bacteriol 26: 401-408.
• Hanselman BA, Kruth S, Weese JS (2007) Methicillin-resistant staphylococcal colonization in dogs entering a veterinary teaching hospital. Vet Microbiol 126: 277-281.
• Hartmann FA, White DG, West SE, Walker RD, Deboer DJ (2005) Molecular characterization of Staphylococcus intermedius carriage by healthy dogs and comparison of antimicrobial susceptibility patterns to isolates from dogs with pyoderma. Vet Microbiol 108: 119-131.
• Hauschild T, Wójcik A (2007) Species distribution and properties of staphylococci from canine dermatitis. Res Vet Sci 82: 1-6.
• Kizerwetter-Świda M, Chrobak D, Rzewuska M, Binek M (2009) Antibiotic resistance patterns and occurence of mecA gene in Staphylococcus intermedius strains of canine origin. Pol J Vet Sci 12: 9-13.
• Loeffler A, Linek M, Moodley A, Guardabassi L, Sung JM, Winkler M, Weiss R, Lloyd DH (2007) First report of multiresistant, mecA-positive Staphylococcus intermedius in Europe: 12 cases from a veterinary dermatology referral clinic in Germany. Vet Dermatol 18: 412-421.
• Lyskova P, Vydrzalova M, Mazurova J (2007) Identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa. J Vet Med A Physiol Pathol Clin Med 54: 559-563.
• Mahoudeau I, Delabranche X, Prevost G, Monteil H, Piemont Y (1997) Frequency of isolation of Staphylococcus intermedius from humans. J Clin Microbiol 35: 2153-2154.
• Malayeri HZ, Jamshidi S, Salehi TZ (2010) Identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs. Vet Res Commun 34: 435-444.
• Malicki K, Binek M (2004) Outline of Clinical Veterinary Bacteriology (in Polish). 1st ed., Warsaw University of Life Sciences (SGGW) Press, Warsaw.
• Międzobrodzki J, Kasprowicz A, Białecka A, Jaworska O, Polakowska K, Władyka B, Dublin A (2010) The first case of a Staphylococcus pseudintermedius infection after joint prothesis implantation in a dog. Pol J Microbiol 59: 133-135.
• Oliveira NE, Cardozo AP, Marques Ede A, dos Santos KR, Giambiagi-de Marval M (2007) Interpretive criteria to differentiate low-and high-level mupirocin resistance in Staphylococcus aureus. J Med Microbiol 56: 937-939.
• Oliveira LC, Leite CA, Brilhante RS, Carvalho CB (2008) Comparative study of the microbial profile from bilateral canine otitis externa. Can Vet J 49: 785-788.
• Perreten V, Kadlec K, Schwarz S, Andersson UG, Finn M, Greko C, Moodley A, Kania SA, Frank LA, Bemis DA, Franco A, Iurescia M, Battisti A, Duim B, Wagenaar JA, van Duijkeren E, Weese JS, Fitzgerald JR, Rossano A, Guardabassi L (2010) Clonal spread of methicillin-resistant Staphylococcus pseudintermedius in Europe and North America: an international multicentre study. J Antimicrob Chemother 65: 1145-1154.
• Prescott JF, Hanna WJ, Reid-Smith R, Drost K (2002) Antimicrobial drug use and resistance in dogs. Can Vet J 43: 107-116.
• Ruscher C, Lubke-Becker A, Wleklinski CG, Soba A, Wieler LH, Walther B (2009) Prevalence of methicillin-resistant Staphylococcus pseudintermedius isolated from clinical samples of companion animals and equidaes. Vet Microbiol 136: 197-201.
• Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K (2007) Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital. J Clin Microbiol 45: 1118-1125.
• Strommenger B, Kettlitz C, Werner G, Witte W (2003) Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J Clin Microbiol 41: 4089-4094.
• Tanner MA, Everett CL, Youvan DC (2000) Molecular phylogenetic evidence for noninvasive zoonotic transmission of Staphylococcus intermedius from canine pet to a human. J Clin Microbiol 38: 1628-1631.
• van Duijkeren E, Houwers DJ, Schoormans A, Broekhuizen-Stins MJ, Ikawaty R, Fluit AC, Wagenaar JA (2008) Transmission of methicillin-resistant Staphylococcus intermedius between humans and animals. Vet Microbiol 128: 213-215.
• Vanni M, Tognetti R, Pretti C, Crema F, Soldani G, Meucci V, Intorre L (2009) Antimicrobial susceptibility of Staphylococcus intermedius and Staphylococcus schleiferi isolated from dogs. Res Vet Sci 87: 192-195.
• Vengust M, Anderson ME, Rousseau J, Weese JS (2006) Methicillin-resistant staphylococcal colonization in clinically normal dogs and horses in the community. Lett Appl Microbiol 43: 602-606.
• Wakita Y, Kawano J, Shimizu A, Hajek V, Tomisaka E, Yasuda R, Matsudo E (2002) Development of a PCR test for the identification of Staphylococcus intermedius based on the 16S rDNA sequence. J Vet Med Sci 64: 603-605.
• Wettstein K, Descloux S, Rossano A, Perreten V (2008) Emergence of methicillin-resistant Staphylococcus pseudintermedius in Switzerland: three cases of urinary tract infections in cats. Schweiz Arch Tierheilkd 150: 339-343.
• Zubeir IE, Kanbar T, Alber J, Lämmler C, Akineden O, Weiss R, Zscho¨ck M (2007) Phenotypic and genotypic characteristics of methicillin/oxacillin-resistant Staphylococcus intermedius isolated from clinical specimens during routine veterinary microbiological examinations. Vet Microbiol 121: 170-176.