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2009 | 69 | 3 |
Tytuł artykułu

Study of the role of alpha-synuclein phosphorylation at Ser 129 in dopaminergic neurodegeneration in parkinson's disease

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Studies during the last decade have established a central role in Parkinson’s disease (PD) neurodegeneration for the protein alphasynuclein (α-syn). Among the different post-translational modifi - cations that human a-syn can undergo, phosphorylation has been related to the disease. α-syn phosphorylation at Ser 129 seems to be related to the disease progress in PD, since S129P-α-syn is found in low levels in healthy humans and it is related to increased inclusion formation. In order to elucidate the role of phosphorylation of α-syn at Ser 129 in the disease process we used a rat PD model based on the local injection of recombinant adeno-associated viral vectors (rAAV). We created mutations in α-syn at Ser129, replacing the serine either with alanine (S129A) to block phosphorylation or with aspartate (S129D) to mimic phosphorylation. We overexpressed wt, S129A and S129D α-syn in rat midbrain and thereafter have compared the effect of the overexpression on dopaminergic neurons at different time points post-injection. Our results show that pseudophosphorylated α-syn S129D induced faster motor defi cit compared to S129A. On our hands, the phosphorylation of α-syn at Ser129 seems to have a role in the progression of the disease but is not necessary for pathology, motor defi cits or cell death to occur.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.343
Twórcy
autor
  • Department of Medical Biochemistry, Aarhus University, Aarhus, Denmark
autor
  • Department of Medical Biochemistry, Aarhus University, Aarhus, Denmark
autor
  • B.R.A.I.N.S. Department of Experimental Medical Science, Lund University, Lund, Sweden
  • Department of Medical Biochemistry, Aarhus University, Aarhus, Denmark
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-6ef3282e-d676-441e-8866-24acb9fd7ea0
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