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2009 | 69 | 3 |
Tytuł artykułu

HUCB-NSCS growth control by biofunctionalized surfaces

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cell growth platforms with biofunctionalized surfaces were fabricated to control and direct HUCB-NSC fate decisions. Two different nano/micro techniques: microcontact printing and piezoelectric non-contact spotting were used to allocate biomolecules (poly-Llysine and fi bronectin) on cell-repellent, non adhesive substrate. Such methods allow controlling the spatial distribution and content of the biomolecules on the microarray and governing cell adhesion in unspecifi c (electrostatic) or specifi c (receptor-mediated) manner. Patterning of biomolecules in different conditions on the single growth platforms enables to infl uence and compare stem cells developmental processes (proliferation /differentiation) at variable environments. To refl ect/mimic stem cell niche we applied functional domains containing ECM protein spotted together with the small signaling molecules (notch, wnt, shh). Such approach enable directing of neural stem cell developmental program by inducing intracellular molecular pathways leading to either self renewing- or differentiating- (neuronal or astrocytic) state. We characterized active biofunctionalized domains on fabricated microarrays by applying two methods of surface analysis: ellipsometry measurement and surface plasmon resonance system. This type of bioengineered cell growth platforms can be used for screening the mechanisms governing neural stem cell fate decisions and adverse reactions upon environmental stimuli. Grant No 0141/B/ P01/2008/35.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.294
Twórcy
autor
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • In Vitro Toxicology Methods Unit, Institure fo Health and Consumer Protection, European Commission, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
autor
  • Nanobiosciences Unit, Joint Research Centre, Ispra, Italy
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
autor
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-6b2ed5d8-c956-4231-ab9a-b43337a56f02
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