Stress-induced delta1-pyrroline-5-carboxylate synthetase (P5CS) gene confers tolerance to salt stress in transgenic sugarcane

• Autorzy: Guerzoni J.T.S. , Belintani N.G. , Moreira R.M.P. , Hoshino A.A. , Domingues D.S. , Filho J.C.B. , Vieira L.G.E.
• Języki publikacji: EN

Abstrakty

EN

High salinity interferes in sugarcane growth and development, affecting not only crop yield but also reducing sucrose concentration in culms. Sugarcane plants submitted to salt stress can accumulate compatible solutes, such as proline, which may counteract the effects of salt accumulation in the vacuole and scavenge reactive oxygen species. The objective of this study was to evaluate the response to salt stress of sugarcane plants transformed with the Vigna aconitifolia P5CS gene, which encodes Δ1- pyrroline-5-carboxylate synthetase, under the control of a stress-induced promoter AIPC (ABA-inducible promoter complex). For this, 4-month-old clonally multiplied sugarcane plants from two transformation events were irrigated every 2 days with 1/10 Hoagland’s solution supplemented with 100, 150 and 200 NaCl, progressively, during 28 days. Transgenic lines showed increased transgene expression in 3.75-fold when compared with the control plants after 9 days of irrigation with saline water, which can explain the higher proline concentration found in these plants. At the end of the experiment (day 28), the transgenic lines accumulated up to 25 % higher amounts of proline when compared with non-transformed control plants. Stress response in transgenic plants was also accompanied by a reduction of malondialdehyde (MDA) derived from cellular lipid peroxidation in leaves, lower Na⁺ accumulation in leaves and maintenance of photochemical efficiency of PSII. Thus, proline contributed to the protection of the photosynthetic apparatus and the prevention of oxidative damage in transgenic sugarcane under salt stress.

Słowa kluczowe

EN

plant stress; delta-1-pyrroline-5-carboxylate synthetase; p5cs gene; plant tolerance; salt stress; transgenic plant; sugar-cane

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 09
• Opis fizyczny: p.2309-2319,fig.,ref.

Twórcy

autor

Guerzoni J.T.S.

• Plant Biotechnology Laboratory, Instituto Agronomico do Parana (IAPAR), CP 481, Londrina, PR CEP 86001-970, Brazil
• Graduation Program Agronomy, Sector de Ciencias Agrarias, Universidade Federal do Parana (UFPR), Curitiba, PR, Brazil

autor

Belintani N.G.

• Plant Biotechnology Laboratory, Instituto Agronomico do Parana (IAPAR), CP 481, Londrina, PR CEP 86001-970, Brazil

autor

Moreira R.M.P.

• Departamento de Biologia Geral, Centro de Ciencias Biologicas, Universidade Estadual de Londrina (UEL), Londrina, PR, Brazil

autor

Hoshino A.A.

• Plant Biotechnology Laboratory, Instituto Agronomico do Parana (IAPAR), CP 481, Londrina, PR CEP 86001-970, Brazil

autor

Domingues D.S.

• Plant Biotechnology Laboratory, Instituto Agronomico do Parana (IAPAR), CP 481, Londrina, PR CEP 86001-970, Brazil

autor

Filho J.C.B.

• Plant Biotechnology Laboratory, Instituto Agronomico do Parana (IAPAR), CP 481, Londrina, PR CEP 86001-970, Brazil

autor

Vieira L.G.E.

• Graduation Program in Agronomy, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, SP, Brazil

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Identyfikatory

DOI

10.1007/s11738-014-1579-8