Virulence factors in coagulase-negative staphylococci isolated from cows with subclinical mastitis

• Autorzy: Malinowski E. , Klossowska A. , Zastempowska E.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the presence of virulence genes in 46 coagulase-negative staphylococci (CNS) strains isolated from 46 udder quarters of 46 cows with subclinical mastitis. The isolates were examined phenotypically (cultural features, Gram stain, catalase, coagulase, API tests) and genotypically for: icaA, icaAB, icaD, atlE, bhp, agrA, sarA, mecA, and blaZ genes. These isolates had no nuc and coa genes, and were identified as S. warneri (11 strains), S. chromogenes (10), S. xylosus (seven), S. epidermidis (one), S. hyicus (one), and other Staphylococcus species (16). The single virulence genes or their combinations were detected in nine (19.6 %) of isolates. The following genes were found: blaZ (in four isolates), mecA (3), sarA (3), agrA (3), and atlE (three). None of the strains harboured the bhp, icaA, icaD, and icaAB genes. In conclusion, subclinical mastitis in cows can be caused by different species of CNS that harbour different virulence factors.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.681-684,ref.

Twórcy

autor

Malinowski E.

• Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, 85-090 Bydgoszcz, Poland

autor

Klossowska A.

autor

Zastempowska E.

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