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2013 | 35 | 06 |

Tytuł artykułu

A Bowman–Birk protease inhibitor with antifeedant and antifungal activity from Dolichos biflorus

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
In the present study, 11 varieties of Dolichos biflorus exhibited both protease inhibitor activities as well as in vitro inhibitory activity against Helicoverpa armigera gut protease. A Bowman–Birk protease inhibitor showing activity against trypsin and α-chymotrypsin has been purified from D. biflorus seeds using multi-step strategy. The purified inhibitor revealed a single band on SDSPAGE corresponding to molecular mass of 16 kDa. The inhibitory constants for the interaction of purified PI with trypsin and α-chymotrypsin were 0.04 and 0.48 lM, respectively. The purified inhibitor was stable over a pH range of 2–12 and up to a temperature of 100 ºC for 20 min. The results of insect bioassay against H. armigera revealed 68 % decline in larval weight after 7 days of feeding on artificial diet containing the inhibitor. The larval growth and % leaf area eaten were drastically reduced in the presence of inhibitor. The observed cumulative mortality from larval to adult was 51.21 %. The inhibitor displayed antifungal activity against Alternaria alternata, Fusarium oxysporum, and Aspergillus niger with minimum inhibitory concentration as 0.4, 0.6, and 1.2 μg mL-1, respectively. This is the first report of anti-feedant and anti-fungal activities of D. biflorus protease inhibitor on a single protein, which might be important for developing transgenic plants resistant to insect pests and fungal pathogens.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

06

Opis fizyczny

p.1887-1903,fig.,ref.

Twórcy

autor
  • Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India
autor
  • National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute (PUSA), New Delhi, India
  • Division of Entomology, Indian Agricultural Research Institute (PUSA), New Delhi, India
  • National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute (PUSA), New Delhi, India
autor
  • Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India
autor
  • Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India

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Bibliografia

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