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2014 | 63 | 1 |

Tytuł artykułu

Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
This study was carried out to determine the effects of novobiocin, a gyrase inhibitor, on the growth, survival, motility and whole cell proteins of S. Typhimurium dam and/or seqA strains. Our results showed that the dam and seqA/dam mutants are the most sensitive to novobiocin, compared to wild type and seqA strains. Surprisingly, the motility of seqA mutants increased after exposure to novobiocin only in stationary phase cells. All the other strains showed a significant decrease in their motility. The analysis of protein profiles of all strains demonstrated several modifications as manifested by the alteration of the expression levels of certain bands. Our work is therefore of great interest in understanding the effects of novobiocin on S. Typhimurium and the involvement of DNA methylation.

Wydawca

-

Rocznik

Tom

63

Numer

1

Opis fizyczny

p.51-56,fig.,ref.

Twórcy

autor
  • Laboratoire de traitement des eaux usees, Centre des Recherches et Technologies des Eaux, Technopole Borj-cedria, BP 273- Soliman 8020, Tunisie
autor
  • Laboratoire de Biochimie et Biologie moleculaire, Faculte des Sciences de Bizerte, Zarzouna 7021, Universite de Carthage, Tunisie
autor
  • Laboratoire de Biochimie et Biologie moleculaire, Faculte des Sciences de Bizerte, Faculte des Sciences de Bizerte, Zarzouna 7021, Universite de Carthage, Tunisie
autor
  • Laboratoire de Biochimie et Biologie moleculaire, Faculte des Sciences de Bizerte, Faculte des Sciences de Bizerte, Zarzouna 7021, Universite de Carthage, Tunisie
autor
  • Laboratoire de Biochimie et Biologie moleculaire, Faculte des Sciences de Bizerte, Faculte des Sciences de Bizerte, Zarzouna 7021, Universite de Carthage, Tunisie

Bibliografia

  • Badie G., D.M. Heithoff, R.L. Sinsheimer and M.J. Mahan. 2007. Altered levels of Salmonella DNA adenine methylase are associated with defects in gene expression, motility, flagellar synthesis, and bile resistance in the pathogenic strain 14028 but not in the laboratory strain LT2. J. Bacteriol. 189: 1556–1564.
  • Balbontín R., G. Rowley, M.G. Pucciarelli, J. López-Garrido,Y. Wormstone, S. Lucchini, F. García-del Portillo, J.C.D. Hinton and J. Casadesús. 2006. DNA adenine methylation regulates virulence gene expression in Salmonella enterica serovar Typhimurium. J. Bacteriol. 188: 8160–8168.
  • Boye E., T. Stokke, N. Kleckner and K. Skarstad. 1996. Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins. Proc. Natl. Acad. Sci. USA 93: 12206–12211.
  • Chatterji M., S. Unniraman, S. Mahadevan and V. Nagaraja. 2001. Effect of different classes of inhibitors on DNA gyrase from Mycobacterium smegmatis. J. Antimicrob. Chemother. 48: 479–485.
  • Chatti A., D. Daghfous and A. Landoulsi. 2007. Effect of seqA mutation on Salmonella typhimurium virulence. J. Infect. 54: e241–e245.
  • Chatti A. and A. Landoulsi. 2008. The DNA-methylation state regulates virulence and stress response of Salmonella. C.R. Biol. 331: 648–654.
  • Chatti A., N. Messaoudi, M. Mihoub and A. Landoulsi. 2012. Effect of hydrogen peroxide on the motility, catalase and superoxide dismutase of dam and/or seqA mutant of Salmonella Typhimurium. World J. Microbiol. Biotechnol. 28: 129–33.
  • Dove S.L. and C.J. Dorman. 1994. The site-specific recombination system regulating expression of the type 1 fimbrial subunit gene of Escherichia coli is sensitive to changes in DNA supercoiling. Mol. Microbiol. 14:975–988.
  • Gellert M., M.H. O’dea, T. ITOH and J-I. Tomizawa. 1976. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc. Natl. Acad. Sci. USA 73: 4474–4478.
  • Heithoff D.M., R.L. Sinsheimer, D.A. Low and M.J. Mahan. 1999. An essential role for DNA adenine methylation in bacterial virulence. Science 284: 967–970.
  • Jakomin M., D. Chessa, A.J. Baumler and J. Casadesus. 2008. Regulation of the Salmonella enterica std Fimbrial Operon by DNA Adenine Methylation, SeqA, and HdfR. J. Bacteriol. 190: 7406–7413.
  • Klungsøyr H.K. and K. Skarstad. 2004. Positive supercoiling is generated in the presence of Escherichia coli SeqA protein. Mol. Microbiol. 54: 123–31.
  • Lobner-Olesen A., M. Marinus and F. Hansen. 2003. Role of SeqA and Dam in Escherichia coli gene expression: a global microarray analysis. Proc. Natl. Acad. Sci. USA 100: 4672–4677.
  • Lowry O.H., N.J. Rosenbrough and A.L. Farr. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
  • Lu M., J.L. Campbell, E. Boye and N. Kleckner. 1994. SeqA: a negative modulator of replication initiation in E. coli. Cell 77: 413–426.
  • Norunn K.T. and K. Skarstad. 1999. Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC. EMBO J. 18: 4882–4888.
  • Onogi T., M. Yamazoe, C. Ichinose, H. Niki and S. Hiraga. 2000. Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants. J. Bacteriol. 182: 5898–5901.
  • Oshima T., C. Wada, Y. Kawagoe, T. Ara, M. Maeda, Y. Masuda, S. Hiraga and H. Mori. 2002. Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli. Mol. Microbiol. 45: 673–675.
  • Slater S., S. Wold, M. Lu, E. Boye, K. Skarstad and N. Kleckner. 1995. E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration. Cell 82: 927–926.
  • Schneider R., A. Travers and G. Muskhelishvili. 2000. The expression of the Escherichia coli fis gene is strongly dependent on the superhelical density of DNA. Mol. Microbiol. 38: 167–176.
  • Smith D.H. and B.D. Davis. 1967. Mode of Action of Novobiocin in Escherichia coli. J. Bacteriol. 93:71–79
  • Soutourina O.A., E.A. Semenova, V.V. Parfenova, A. Danchin and P. Bertin. 2001. Control of Bacterial Motility by Environmental Factors in Polarly Flagellated and Peritrichous Bacteria Isolated from Lake Baikal. App. Environ. Microbiol. 67: 3852–3859.
  • Soutourina O.A., E. Krin, C. Laurent-Winter, F. Hommais, A. Danchin and P.N. Bertin. 2002. Regulation of bacterial motility in response to low pH in Escherichia coli: the role of H-NS protein. Microbiology 148: 1543–1551.
  • Traversa A., R. Schneider and G. Muskhelishvili. 2001. DNA supercoiling and transcription in Escherichia coli : The FIS connection. Biochimie 83:213−217.
  • Wang J.C. and A.S. Lynch. 1993. Transcription and DNA supercoiling. Curr. Opin. Genet. Dev. 3: 764–768.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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