The correlation analysis of WWOX expression and cancer related genes in neuroblastoma - a real time RT - PCR study

• Autorzy: Nowakowska M. , Pluciennik E. , Wujcicka W. I. , Sitkiewicz A. , Kazanowska B. , Zielinska E. , Bednarek A. K.
• Języki publikacji: EN

Abstrakty

EN

 Neuroblastoma is one of the most common paediatric cancers, described as unpredictable due to diverse patterns of behaviour. WWOX is a tumour suppressor gene whose expression is reduced in many tumour types. Loss of its expression was shown to correlate with more aggressive disease stage and mortality rate. The aim of this study was to investigate the role of the WWOX tumour suppressor gene in neuroblastoma formation. We performed real-time RT-PCR to analyse levels of WWOX expression in 22 neuroblastic tumour samples in correlation with genes involved in cell cycle regulation (CCNE1, CCND1), proliferation (MKI67), apoptosis (BCL2, BIRC5, BAX) and signal transduction (EGFR, ERBB4). We also evaluated two potential mechanisms - promoter methylation (MethylScreen method) and loss of heterozygosity (LOH) status, which could be connected with regulation of WWOX gene expression. We found a positive correlation between WWOX gene and BCL2 and HER4 JM-a and negative with cyclin D1 and E1. Our observations are consistent with previous findings and emphasise the role of WWOX in cell cycle and apoptosis regulation. Moreover, strong positive association with HER4 JM-a in this tumour type may indicate a role for WWOX in neuroblastoma cell differentiation. The presented results indicate that LOH in locus D16S3096 (located in intron 8) may be involved in the regulation of WWOX mRNAexpression. However, no association between methylation status of WWOX promoter and its expression was observed.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2014
• Tom: 61
• Numer: 1
• Opis fizyczny: p.91-97,fig.,ref.

Twórcy

autor

Nowakowska M.

• Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland

autor

Pluciennik E.

• Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland

autor

Wujcicka W. I.

• Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland

autor

Sitkiewicz A.

• Department of Pediatric Surgery and Oncology, Medical University of Lodz, Lodz, Poland

autor

Kazanowska B.

• Department of Bone Marrow Transplantation, Pediatric Oncology, and Hematology, University of Medicine, Wroclaw, Poland

autor

Zielinska E.

• Department of Pediatric Oncology and Hematology, Medical University of Lodz, Lodz, Poland

autor

Bednarek A. K.

• Department of Molecular Cancerogenesis, Medical University of Lodz, Lodz, Poland

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