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2015 | 18 | 3 |
Tytuł artykułu

The experimental osteoporosis in sheep - clinical approach

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The implementation of new methods of oseoporotic therapy requires tests on an animal model. One of the best is the sheep, whose numerous advantages over other models are described in the literature. The aim of this study was induction of osteoporosis using steroids and ovariorectomy methods in sheep and description of the change in parameters with regard to healthy sheep. The study was performed on female „merino” breed sheep divided into three groups: Negative control (NC) healthy animals, positive control (PC) ovariorectomised animals and steroid group (SC) where methylprednisolone was implemented. Blood tests, diagnostic arthroscopy, quantitative computed tomography and X-Ray micro-tomography of bone were carried out. Blood tests revealed a decreased level of estrogens, progesterone and increased parathormone and cortisol levels in the SC group. A decrease in bone turnover markers and an increase in bone resorption markers in all groups were also noted. Diagnostic arthroscopy revealed osteoarthrosis in PC and SC groups. Radiological density tests showed a slight decrease in PC and NC groups whereas there was more than a triple decrease in SC. Results obtained from microCT showed quickly developing osteoporosis in the SC group, which is reflected in numerous parameters analysed in this study. The best effects for osteoporosis induction were obtained using ovariorectomised sheep with methylprednisolone injections.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
3
Opis fizyczny
p.645-654,fig.,ref.
Twórcy
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
autor
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
autor
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
autor
  • Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
autor
  • Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wroclaw, Wroclaw, Poland
autor
  • Department of Biomedical Engineering and Experimental Mechanics, Wroclaw University of Technology, Wroclaw, Poland
autor
  • Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, 50-370 Wroclaw, Poland
autor
  • Department of Biomedical Engineering and Experimental Mechanics, Wroclaw University of Technology, Wroclaw, Poland
Bibliografia
  • An YH, Gruber HE (2003) Introduction to Experimental Bone and Cartilage Histology. In: An YH, Martin KL (eds) Handbook of Histology Methods for Bone and Cartilage, 1st ed., Totowa, NJ, Human Press Inc, pp 3-31.
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  • Peichl P, Griesmacher A, Marteau R, Hejc S, Kumpan W, Muller MM, Broll H (2001) Serum crosslaps in comparison to serum osteocalcin and urinary bone resorption markers. Clin Biochem 34: 131-139.
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  • Roman-Bias JA, Castaneda S, Largo R, Herrero-Beaumont G (2009) Osteoarthritis associated with estrogen deficiency. Arthritis Res Ther 11: 241.
  • Schorlemmer S, Gohl Ch, Iwabu S, Ignatius A, Claes Lutz, Augat P (2003) Glucocorticoid Treatment of Ovariectomized Sheep Affects Mineral Density, Structure, and Mechanical Properties of Cancellous Bone. Bone Miner Res 18: 1010-1015.
  • Sigrist IM, Gerhardt C, Alini M, Schneider E, Egermann M (2007) The long-term effects of ovariectomy on bone metabolism in sheep. J Bone Miner Metab 25: 28-35.
  • Sweigart MA, Athanasiou KA (2004) Biomechanical characteristics of the normal medial and lateral porcine knee menisci. Proc I Mech E 219 Part H: JEngineering in Medicine.
  • Turner AS, Alvis M, Myers W, Stevens ML, Lundy MW (1995) Changes in bone mineral density and bone- alkaline phosphatase in ovariectomized ewes. Bone 17: 395-402.
  • Turner AS (2002)The sheep as a model for osteoporosis in humans. The Veterinary Journal 163: 232-239.
  • Uklejewski R, Winiecki M, Rogala P (2006) Zgodność strukturalno-adaptacyjna połączeń kości z porowatymi implantami na podstawie tradycyjnego jednofazowego I współczesnego dwufazowego porosprężystego modelu biomechaniczngo tkanki kostnej. Engineering of Biomaterials 1-13: 54-55
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-63814bd5-4729-4755-b67f-af1770c23577
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