Preliminary assessment of ELISA, MAT, and LAT for detecting Toxoplasma gondii antibodies in pigs

• Autorzy: Sroka J. , Cencek T. , Ziomko I. , Karamon J. , Zwolinski J.
• Języki publikacji: EN

Abstrakty

EN

Applicability and sensitivity of the commercial tests MAT (modified agglutination test), LAT (latex agglutination test), in comparison to in-house serologic assay (ELISA), and reference test (indirect fluorescence antibody test, IFAT) to detect specific antibodies against Toxoplasma gondii in pigs were investigated. One hundred and six blood samples were examined. The most specific test, as compared to IFAT, was MAT (100.0%) and the most sensitive test was ELISA (88.9%). LAT showed the least specificity and sensitivity at 91.4%, and 47.2%, respectively. After analysis of the tests with Cohen's Kappa agreement, it was found that IFAT, ELISA and MAT were in high agreement, and LAT was moderately consistent with these tests. Similar results were achieved using Spearman's rank correlation coefficient. Our study confirmed the usefulness of the ELISA and MAT, much greater than LAT, for the detecting of T. gondii antibodies in pigs.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.545-549,fig.,ref.

Twórcy

autor

Sroka J.

• Department of Parasitology, National Veterinary Research Institute, 24-100 Pulawy, Poland
• Department of Occupational Biohazards, Institute of Agricultural Medicine, 20-950 Lublin, Poland

autor

Cencek T.

• Department of Parasitology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Ziomko I.

• Department of Parasitology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Karamon J.

• Department of Parasitology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zwolinski J.

• Department of Occupational Biohazards, Institute of Agricultural Medicine, 20-950 Lublin, Poland

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