Somatic embryogenesis from immature male flowers and molecular analysis of regenerated plants in banana "Lal Kela" (AAA)

• Autorzy: Meenakshi S. , Shinde B. N. , Suprasanna P.

Warianty tytułu

PL

Embriogeneza somatyczna z niedojrzalych kwiatow meskich i analiza molekularna zregenerowanych roslin banana odmiany „Lal Kela‟ (AAA)

• Języki publikacji: EN

Abstrakty

EN

Development of embryogenic cultures having high regeneration efficiency from important, commercial varieties of banana is a prerequisite for genetic manipulation and for in vitro propagation. In the present study, we have studied the induction of somatic embryogenesis from young immature male inflorescences of the banana cul­tivar Lal Kela (red banana) on M2 medium (Ma et al., 1991) supplemented with 2,4-D. Cell suspension cultures initiated from this callus exhibited embryogenic stages. So­matic embryos developed into plantlets on / strength MS basal medium with 100 mg l-1 ME + 0.1% AC and 0.2% gelrite. Plants regenerated through somatic embryogenesis showed minor variation when assessed by randomly amplified polymorphic DNA (RAPD) and sequence characterized amplified regions (SCAR) markers.

PL

Rozwój kultur embriogenicznych mających wysoką zdolność regeneracyjną i po­chodzących z odmian bananów ważnych pod względem handlowym, jest niezbędny do manipulacji genetycznej i rozmnażania in vitro. Badania miały na celu indukcję somatycznej embriogenezy z męskich niedojrzałych kwiatostanów banana odmiany Lal Kela (czerwony banan) wyszczepionych na pożywce M2 (Ma i in., 1991) wzbo­gaconej 2,4-D. Zawiesina kultur komórkowych powstała z wytworzonego kalusa wy­tworzyła wczesne stadia embryonalne. Zarodki somatyczne rozwinęły się w roślinki na pożywce podstawowej MS dwukrotnie i uzupełnionej 100 mg l-1 ME + 0.1% AC i 0.2% gelritu. Rośliny zregenerowane w wyniku embriogenezy somatycznej wykaza­ły niewielkie zróżnicowanie, które określone było z użyciem markerów RAPD i SCAR

Słowa kluczowe

EN

somatic embryogenesis; male flower; molecular analysis; banana; Musa; Lel Kela cultivar; genetic fidelity; plant regeneration; randomly amplified polymorphic DNA; sequence characterized amplified region

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2011
• Tom: 19
• Numer: 2
• Opis fizyczny: p.15-30,fig.,ref.

Twórcy

autor

Meenakshi S.

• Department of Horticulture, Marathwada Agricultural University, Parbhani, India

autor

Shinde B. N.

autor

Suprasanna P.

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