Cytogenetic analysis of MSRV pol, gag, env sequences and genome instability in multiple sclerosis

• Autorzy: Zawada M. , Liwen I. , Januszkiewicz-Lewandowska D. , Nowicka K. , Jaworska-Kubicka B. , Rembowska I. , Hetmanowska K. , Wander W. , Nowak J.
• Języki publikacji: EN

Abstrakty

EN

Among of the potential agents causing multiple sclerosis MSRV virus (multiple sclerosis-associated retrovirus) is often taken into consideration. Aims of the study were (1) an assessment of MSRV potential role in MS, and (2) test of genome instability in MS patients. The material was peripheral blood lymphocytes from 92 patients with MS, 12 patients with myasthenia and 20 healthy persons. The FISH studies with labeled PCR products of pol gag and env MSRV genes in nuclei, chromosomes and chromatin fi - bers were done. Classical cytogenetic techniques were introduced into karyotypes and micronuclei analyses. MSRV pol, gag and env sequences were found in both MS patients and controls. The copy number of MSRV pol sequence was signifi cantly greater in MS patients (6ñ24 copies on nucleus) than in myasthenia (4ñ5 copies) and normal individuals (3ñ6 copies). MSRV gag sequence was found in a range of 5ñ20, 4ñ5, and 2ñ4 copies in MS patients, patients with myasthenia and healthy donors, respectively. MSRV env was found in a range of 6ñ22, 4ñ5, and 2ñ4 copies in MS patients, patients with myasthenia and healthy donors, respectively. Moreover, the number of spontaneous micronuclei was signifi cantly greater in MS patients compared to control. In patients with MS diversity of chromosome aberrations was observed. In conclusion, evident difference in MSRV pol, gag and env copy number between MS patients and control suggests that MSRV may play some role in the etiology of multiple sclerosis (latent viral infection). The presence of chromosome aberrations and high amount of micronuclei in MS patients shows that the instability in MS genome often occurs. Scientifi c work has been supported by Ministry of Scientifi c Research and Information Technology funds (Grant No 2 PO5A 139 28).

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.89-90

Twórcy

autor

Zawada M.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Liwen I.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Januszkiewicz-Lewandowska D.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
• University of Medical Sciences, Poznan, Poland

autor

Nowicka K.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Jaworska-Kubicka B.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Rembowska I.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

autor

Hetmanowska K.

• Department of Neurology, State Hospital, Poznan, Poland

autor

Wander W.

• Neuroimmunological Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, Poznan, Poland

autor

Nowak J.

• Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland