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2015 | 18 | 3 |
Tytuł artykułu

Cytomorphometry of serosal effusion in dogs

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Cytomorphometry made on cytological slides is the quantitative method of precise analysis of cellular structures, including both cytoplasm and nucleus. The aim of this study was to describe cytomorphometric parameters of mesothelial cells in the course of benign reactive and malignant proliferation and to compare them to carcinomas and adenocarcinomas located within serosal cavities in dogs. The second aim was to evaluate applicability of cytomorphometry to diagnostics of diseases causing accumulation of effusion in serosal cavities. Cytological samples of normal and non-malignant mesothelium, mesothelioma and various carcinomas were collected from dogs. Cytomorphometry was made on the smears stained with Giemsa solution. Mean nuclear and cellular perimeter, mean nuclear and cellular area, mean nuclear and cellular diameter, and mean nuclear and cellular roundness were determined. Moreover, nuclear to cytoplasmic ratio (N/C) was calculated. The data revealed statistically significant differences for all parameters, excluding mean nuclear perimeter, between compared groups. Normal mesothelium cells and their nuclei were significantly smaller and more elongated than cells and nuclei of both benign reactive and malignant neoplastic mesothelium. Only a few differences were observed between benign reactive mesothelium cells and mesothelioma cells – mean nuclear area and mean nuclear diameter of benign reactive mesothelium cells were significantly larger and N/C ratio was higher in comparison to mesothelioma cells. Even though some significant differences were observed, considerable overlap of these cytomorphometric parameters in animals with different diseases limited practical role of these observations. Cytomorphometric analysis of cellular samples collected from dogs with proliferative processes affecting serosal cavities can be only an auxiliary method increasing accuracy of preoperative diagnosis.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
3
Opis fizyczny
p.481-487,fig.,ref.
Twórcy
  • Department of Pathology and Veterinary Diagnostics, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland
autor
  • Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland
  • Department of Pathology and Veterinary Diagnostics, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland
Bibliografia
  • Baker R, Lumsden JH. Pleural and peritoneal fluids. In: Baker R, Lumsden JH (2000) Color atlas of cytology of the dog and cat. Mosby, St. Louis, pp 161-162.
  • Barbosa DFL, Paraventi MD, Strefezzi RF (2014) Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors – intra- and interobserver variability. Vet Clin Pathol 43: 469-472.
  • Bertazzolo W, Bonfanti U, Mazzotti S, Gelain ME (2012) Cytologic features and diagnostic accuracy of analysis of effusions for detection of ovarian carcinoma in dogs. Vet Clin Pathol 41: 127-132.
  • Brisson BA, Reggeti F, Bienzle D (2006) Portal site metastasis of invasive mesothelioma after diagnostic thoracoscopy in a dog. J Am Vet Med Assoc 229: 980-983.
  • Charney SC, Bergman PJ, McKnight JA, Ferelly J, Novosad CA, Leibman NF, Camps-Palau MA (2005) Evaluation of intracavitary mitoxantrone and carboplatin for treatment of carcinomatosis, sarcomatosis and mesothelioma, with or without malignant effusions: A retrospective analysis of 12 cases (1997-2002). Vet Comp Oncol 3: 171-181.
  • De Vico G, Sfacteria A, Maiolino P, Mazzullo G (2002) Comparison of nuclear morphometric parameters on cytologic smears and histologic sections of spontaneous canine tumors. Vet Clin Pathol 31: 16-18.
  • De Vico G, Maiolino P, Cataldi M, Mazzullo G, Restucci B (2007) Nuclear morphometry in relation to lymph node status in canine mammary carcinomas. Vet Res Commun 31: 1005-1011.
  • Geninet C, Bernex F, Rakotovao F, Crespeau FL, Parodi AL, Fontaine JJ (2003) Sclerosing peritoneal mesothelioma in a dog – a case report. J Vet Med A 50: 402-405.
  • Glińska K (2009) Evaluation of the usefulness of abdominal fluid examination in diagnosing the etiology of ascites in dogs. Med Weter 65: 40-45.
  • Gumber S, Fowlkes N, Cho DY (2011) Disseminated sclerosing peritoneal mesothelioma in a dog. J Vet Diagn Invest 23: 1046-1050.
  • Maiolino P, Restucci B, Papparella S, De Vico G (2002) Nuclear morphometry in squamous cell carcinomas of canine skin. J Comp Pathol 127: 114-117.
  • Ordonez NG (2006) The diagnostic utility of immunohistochemistry and electron microscopy in distinguishing between peritoneal mesotheliomas and serous carcinomas: a comparative study. Mod Pathol 19: 34-48.
  • Paździor-Czapula K, Otrocka-Domagała I, Rotkiewicz T, Gesek M (2014) Cytomorphometry of canine cutaneous histiocytoma. Pol J Vet Sci 17: 413-420.
  • Przeździecki R, Sapierzyński R (2014) Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs. Pol J Vet Sci 17: 149-159.
  • Sato T, Miyoshi T, Shibuya H, Fujikura J, Koie H, Miyazaki Y (2005) Peritoneal biphasic mesothelioma in a dog. J Vet Med A 52: 22-25.
  • Simeonov R, Simeonova G (2006) Computerized morphometry of mean nuclear diameter and nuclear roundness in canine mammary gland tumors on cytologic smears. Vet Clin Pathol 35: 88-90.
  • Simeonov R, Simeonova G (2007a) Computerized cytomorphometric analysis of nuclear area, nuclear perimeter and mean nuclear diameter in spontaneous canine mammary gland tumours. Vet Res Commun 31: 553-558.
  • Simeonov RS, Simeonova GP (2007b) Use of quantitative analysis as a method for differentiation between canine cutaneous apocrine adenomas and carcinomas on cytologic smears. J Vet Med A 54: 542-544.
  • Simeonov R, Simeonova G (2008) Quantitative analysis in spontaneous canine anal sac gland adenomas and carcinomas. Res Vet Sci 85: 559-562.
  • Simeonov R, Simeonova G (2009) Nuclear cytomorphometry in feline mammary gland epithelial tumours. Vet J 179: 296-300.
  • Simeonov R, Simeonova G (2010) Comparative morphometric analysis of recurrent and nonrecurrent canine basal cell carcinomas: a preliminary report. Vet Clin Pathol 39: 96-98.
  • Smith DA, Hill FW (1989) Metastatic malignant mesothelioma in a dog. J Comp Pathol 100: 97-101.
  • Strefezzi RF, Xavier JG, Kleeb SR, Catao-Dias JL (2009) Nuclear morphometry in cytopathology: a prognostic indicator for canine cutaneous mast cell tumors. J Vet Diagn Invest 21: 821-825.
  • Szczepulska-Wójcik E, Langfort R, Roszkowski-Śliz K (2007) A comparative evaluation of immunohistochemical markers for the differential diagnosis between malignant mesothelioma, non-small cell carcinoma involving the pleura, and benign reactive mesothelial cell proliferation. Pneumonol Alergol Pol 75: 57-69.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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