First molecular characterisation and identification of a Chlamydophila felis from urban cats in Wroclaw, Poland

• Autorzy: Ploneczka-Janeczko K. , Kielbowicz Z. , Kuczkowski M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to investigate the prevalence of Chlamydophila felis infection in cats from Wroclaw city. Fiftytwo cats with ocular signs of chronic conjunctivitis, selected on the basis of anamnesis, ophthalmological examination, and clinical symptoms, were included in the screening study. Using cotton swabs, conjunctival samples were collected from all animals. The samples were analysed for the presence of Chlamydophila felis using PCR and sequencing analysis. Feline DNA was demonstrated in all samples. Samples from two cats (3.8%) were confirmed to contain chlamydial DNA. The results of sequence analysis of the 600-bp OMP2 gene fragment revealed 100% identity with Russian strains of Chlamydophila felis and showed very high similarity with strains from Italy and Great Britain.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 2
• Opis fizyczny: p.193-198,fig.,ref.

Twórcy

autor

Ploneczka-Janeczko K.

• Department of Epizootiology with Clinic for Birds and Exotic Animals, Wroclaw University of Environment and Life Sciences, 50-375 Wroclaw, Poland

autor

Kielbowicz Z.

autor

Kuczkowski M.

Bibliografia

• 1. Bannasch M., Foley J.: Epidemiologic evaluation of multiple respiratory pathogens in cats in animal shelters. J Feline Med Surg 2005, 7, 109-119.
• 2. Cai Y., Fukushi H., Koyasu S., Kuroda E., Yamaguchi T., Hirai K.: An etiological investigation of domestic cats with conjunctivitis and upper respiratory tract disease in Japan. J Med Vet Sci 2002, 64, 215-219.
• 3. Dean R., Harley R., Helps C., Caney S., Gruffyd-Jones T.: Use of quantitative real-time PCR to monitor the response of Chlamydophila felis infection to doxycycline treatment. J Clin Microbiol 2005, 43, 1858-1864.
• 4. Demkin V.V., Zimin A.L.: A new amplification target for PCR-RFLP detection and identification of Chlamydiaceae species. Arch Microbiol 2005, 183, 169- 175, LOCUS AY 286314 submitted 2003 to the EMBL/GenBank/DDBJ databases.
• 5. Di Francesco A., Baldelli R.: PCR amplification and sequencing of the outer membrane protein 2 gene of Chlamydophila felis, (unpublished), LOCUS AF 484215, submitted 2002 to the EMBL/GenBank/DDBJ databases.
• 6. Di Francesco A., Donati M., Carelle M.S., Baldelli R., Cevenini R.: Molecular characterization of a Chlamydophila felis isolate from a cat in Italy. Vet Rec 2004, 154, 239-240, LOCUS AY 184289, submitted 2002 to the EMBL/GenBank/DDBJ databases.
• 7. Di Francesco A., Piva S., Baldelli R.: Prevalence of Chlamydophila felis by PCR among healthy pet cats in Italy. New Microbiol 2004, 27, 199-202.
• 8. Gastón J. Z., Stengel C., Harbour D., Krieger S., Stampf S., Hartmann K.: Prevalence of feline herpesvirus-1, feline calicivirus and Chlamydophila felis in multiple cat households. Kleintierpraxis 2005, 49, 689-698.
• 9. Hartley J.C., Kaye S., Stevenson S., Bennett J., Ridgway G.: PCR detection and molecular identification of Chlamydiaceae species. J Clin Microbiol 2001, 39, 3072-3079, LOCUS AF 367407, submitted 2001 to the EMBL/GenBank/DDBJ databases.
• 10. Hartley J.C., Stevenson S., Robinson A.J., Littlewood J.D., Carder C., Cartledge J., Clark C., Ridgway G.L.: Conjunctivitis due to Chlamydophila felis (Chlamydophila psittaci feline pneumonitis agent) acquired from a cat: case report with molecular characterization of isolates from the patient and cat, (unpublished), LOCUS AF 367406 and AF 367405, submitted 2001 to the EMBL/GenBank/DDBJ databases.
• 11. Holst Ström B., Englund L., Palacios S., Renström L., Berndtsson L.T.: Prevalence of antibodies against feline coronavirus and Chlamydophila felis in Swedish cats. J Feline Med Surg 2006, 8, 207-211.
• 12. Low H.C., Powell C.C., Veir J.K., Hawley J.R., Lappin M.R.: Prevalence of feline herpesvirus 1, Chlamydophila felis, and Mycoplasma spp. DNA in conjunctival cells collected from cats with and without conjunctivitis. Am J Vet Res 2007, 68, 643-648.
• 13. Marsilio F., Di Martino B., Di Francesco C: Use of a duplex-PCR assay to screen for feline herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats. New Microbiol 2004, 27, 287-292.
• 14. McDonald M., Willett B.J., Jarrett O., Addie D.D.: A comparison of DNA amplification, isolation and serology for the detection of Chlamydia psittaci infection in cats. Vet Rec 1998, 143, 97-101.
• 15. Ohya K., Takahara Y., Etsuko Kuroda E., Koyasu S., Hagiwara S., Sakamoto M., Hisaka M., Morizane K., Ishiguro S., Yamaguchi T., Fukushi H.: Chlamydophila felis CF0218 is a novel TMH family protein with potential as a diagnostic antigen for diagnosis of C. felis Infection. Clin Vaccine Immunol 2008, 15, 1606-1615.
• 16. Rampazzo, A., Appino, S., Pregel, P., Tarducci A., Zini, E., Biolatti, B.: Prevalence of Chlamydophila felis and feline herpesvirus 1 in cats with conjunctivitis in Northern I Italy. J Vet Int Med 2003, 17, 799-807.
• 17. Sachse K., Vretou E., Livingstone M., Borel N., Pospischil A., Longbottom D.: Recent developments in the laboratory diagnosis of chlamydial infections. Vet Microbiol 2009, 135, 2-21.
• 18. Siarkou V., Angelopoulou K.: Sequence analysis of the large cystein-rich 60 kDa membrane complex protein gene (omc B) from Greek Chlmaydophila abortus strains, (unpublished), LOCUS DQ 494813, submitted 2006 to I the EMBL/GenBank/DDBJ databases.
• 19. Sjödahl-Essen T., Tidholm A., Thoren P., Person-Wadman A., Bölske G., Aspan A., Treiberg-Berndtsson L.: Evaluation of different sampling methods and results of real-time PCR for detection of feline herpes virus-1, Chlamydophila felis and Mycoplasma felis in cats. Vet Ophthalmol 2008, 11, 375-380.
• 20. Sykes J.E, Anderson G.A., Studdert V.P., Browning G.F.: Prevalence of feline Chlamydia psittaci and feline herpesvirus 1 in cats with upper respiratory tract disease. J Vet Int Med 1999, 13, 153-162.
• 21. Sykes J.E: Feline chlamydophilosis. Clin Tech Small Anim Pract 2005, 20, 129-134.
• 22. von Bomhard W., Polkinghorne A., Lu Z.H., Vaughan L., Vogtlin A., Zimmermann D.R., Spiess B., Pospischil A.: Detection of novel chlamydiae in cats with ocular disease. Am J Vet Res 2003, 64, 1421-1428.
• 23. Wieliczko A.K., Płoneczka-Janeczko K.: Feline herpesvirus 1 and Chlamydophila felis prevalence in cats with chronic conjunctivitis. Polish J Vet Sci 2010, 13, 381-383.