Influence of dietary soy isoflavones on immature hamster uterotrophic and Hershberger assays

• Autorzy: Minta M. , Radko L. , Stypula-Trebas S. , Wozniak B. , Zmudzki J.
• Języki publikacji: EN

Abstrakty

EN

To select appropriate diet for hamsters used in the uterotrophic and Hershberger assays two rodent diets were compared: Murigran (Agropol, Poland) and Altromin 7010 (Altromin Spezialfutter GmbH&Co., Germany). The contents of bioactive compounds in feeds were evaluated by liquid chromatography, and their oestrogenic activity by yeast enhanced green fluorescent protein assay. In opposition to Altromin, Murigran contained high amounts (µg/kg) of genistein (765 600) and daidzein (132 000), and the oestrogenic activity of these compounds, expressed as 17ß-oestradiol equivalent concentration (EEQ), was found to be 9.54 µg EEQ/kg. In in vivo study, Murigran induced a high degree of oestrogenisation in immature hamsters, and females failed to exhibit a normal uterine response to recommended dose of a model oestrogen agonist 17α-ethinyloestradiol. There was no influence of the diet on the weight of five accessory sex organs (ASO): ventral prostate, seminal vesicles with coagulating glands, levator ani bulbocavernosus muscles, Cowper's glands, and glans penis of control males. However, the impact on ASO response to model androgen agonist, testosterone propionate was observed. The obtained results provide the evidence that phytooestrogen-rich feed modulates the oestrogenic and androgenic response to chemicals.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 4
• Opis fizyczny: p.579-585,fig.,ref.

Twórcy

autor

Minta M.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Radko L.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Stypula-Trebas S.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wozniak B.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzki J.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.2478/bvip-2013-0099