Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications

• Autorzy: Kandefer-Gola M. , Madej J.A. , Dzimira S. , Nowak M. , Janus I. , Ciaputa R.
• Języki publikacji: EN

Abstrakty

EN

The study aimed at immunohistochemical analysis of various markers of cell proliferation and comparison of the results with canine mast cell tumours grading systems according to the Patnaik and Kiupel. Tissue sections were stained using classical technique with haematoxylin and eosin, and immunohistochemical studies were performed with Ki-67, PCNA and MCM-3 antibodies. Additionally the mitotic index was assessed. Statistical analysis including rank correlation Spearman's and ANOVA Friedman analysis was performed. The significance was set at p<0.05. Expression of all examined antigens was detected. The results obtained allow concluding that there is a strong relationship between all the cell markers. However, due to the very strong response and positive reaction in the majority of tumours PCNA is not recommended as a prognostic indicator. Ki-67 and MCM-3 can be successfully used in the evaluation of canine mast cell tumours.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 2
• Opis fizyczny: p.241-247,fig.,ref.

Twórcy

autor

Kandefer-Gola M.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Madej J.A.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Dzimira S.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Nowak M.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Janus I.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Ciaputa R.

• Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

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Identyfikatory

DOI

10.1515/pjvs-2015-0031