Report on overcoming the poor quality of ApaI pulsotypes with a short review on PFGE for Listeria monocytogenes

• Autorzy: Kusar D. , Kavalic M. , Ocepek M. , Zdovc I.
• Języki publikacji: EN

Abstrakty

EN

Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared. The obtained pulsotypes showed that restriction digestion with ApaI from New England BioLabs should be complemented with a subsequent overnight incubation of PFGE plugs in TE buffer for better performance.

Słowa kluczowe

EN

overcoming; poor quality; ApaI pulsotype; pulsed-field gel electrophoresis; Listeria monocytogenes; listeriosis; food-borne pathogen

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 3
• Opis fizyczny: p.307-309,fig.,ref.

Twórcy

autor

Kusar D.

• Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

autor

Kavalic M.

• Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

autor

Ocepek M.

• Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

autor

Zdovc I.

• Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

Bibliografia

• Boerlin P. and J.C. Piffaretti. 1991. Typing of human, animal food and environmental isolates of Listeria monocytogenes by multilocus enzyme electrophoresis. Appl. Environ. Microbiol. 57: 1624–1629.
• Carriere C., A. Allardet-Servent, G. Bourg, A. Audurier and M. Ramuz. 1991. DNA polymorphism in strains of Listeria monocytogenes. J. Clin. Microbiol. 29: 1351–1355.
• Cooper K. 2010. PFGE: general overview and troubleshooting tips; Available from: www.pulsenetinternational.org/SiteCollectionDocuments/pfge/PFGE_troubleshooting.pdf; 16th April 2013, date last accessed.
• European Food Safety Authority. 2010. The community summary report on trends and sources of zoonoses, zoonotic agents and foodborne outbreaks in the European Union in 2008. EFSA Journal 8: 1496. Parma, Italy. EFSA; Available from: www.efsa.europa.eu/en/efsajournal/doc/1496.pdf; 16th April 2013, date last accessed.
• Félix B., T.T. Dao, B. Lombard, A. Asséré, A. Brisabois and S. Roussel. 2012. The use of pulsed field gel electrophoresis in Listeria monocytogenes sub-typing – harmonization at the European Union level, pp. 241–254. In: Magdeldin S. (ed). Gel Electrophoresis – Principles and Basics. InTech, Rijeka, Croatia; Available from: www.intechopen.com/books/gel-electrophoresis-principles-andbasics/harmonization-of-listeria-monocytogenes-pfge-sub-typing-at-european-union-level; 16th April 2013, date last accessed.
• Fenlon D.R. 1999. Listeria monocytogenes in the natural environment, pp. 21–37. In: Ryser E.T. and E.H. Marth (eds). Listeria, Listeriosis, and Food Safety. 2nd ed. Marcel Dekker Inc., New York, USA.
• Freeman M. 2011. PFGE: tips and tricks to success and interpretation of results for foodborne outbreak investigations – BioNumerics workshop for PulseNet participants, 15 April 2011; Available from: www.pulsenetinternational.org/SiteCollectionDocuments/bionumerics/15-PFGE_tips_tricks_interpretation2011_MF.pdf; 16th April 2013, date last accessed.
• Freeman M. 2012. 2012 PulseNet PFGE laboratory update: everything old is new again; Available from: www.aphl.org/conferences/proceedings/Documents/2012/2012-PulseNet-OutbreakNet/016-Freeman.pdf; 16th April 2013, date last accessed.
• Giovannacci I., C. Ragimbeau, S. Queguiner, G. Salvat, J.-L. Vendeuvre, V. Carlier and G. Ermel. 1999. Listeria monocytogenes in pork slaughtering and cutting plants: use of RAPD, PFGE and PCRREA for tracking and molecular epidemiology. Int. J. Food Microbiol. 53: 127–140.
• Graves L.M. and B. Swaminathan. 2001. PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int. J. Food Microbiol. 65: 55–62.
• Graves L.M., B. Swaminathan and S.B. Hunter. 2009. Subtyping Listeria monocytogenes, pp. 283–304. In: Ryser E.T. and E.H. Marth (eds). Listeria, Listeriosis, and Food Safety. 3rd ed. CRC Press, Taylor and Francis Group, Boca Raton, FL, USA.
• Halpin J.L., N.M. Garrett, E.M. Ribot, L.M. Graves and K.L. Cooper. 2010. Re-evaluation, optimization, and multilaboratory validation of the PulseNet-standardized pulsed-field gel electrophoresis protocol for Listeria monocytogenes. Foodborne Pathog. Dis. 7: 293–298.
• Hitchins A.D. and R.C. Whiting. 2001. Food-borne Listeria monocytogenes risk assessment. Food Addit. Contam. 18: 1108–1117.
• Hunter S.B., P. Vauterin, M.A. Lambert-Fair, M.S. Van Duyne, K. Kubota, L. Graves, D. Wrigley, T. Barrett and E. Ribot. 2005. Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J. Clin. Microbiol. 43: 1045–1050.
• Painter J. and L. Slutsker. 2007. Listeriosis in humans, pp. 85–109. In: Ryser E.T. and E.H. Marth (eds). Listeria, Listeriosis, and Food Safety. 3rd ed. CRC Press, Taylor and Francis Group, Boca Raton, FL, USA.
• Tompkin R.B. 2002. Control of Listeria monocytogenes in the food-processing environment. J. Food Prot. 65: 709–725.
• Wesley I.V. 2007. Listeriosis in animals, pp. 55–84. In: Ryser E.T. and E.H. Marth (eds). Listeria, Listeriosis, and Food Safety. 3rd ed. CRC Press, Taylor and Francis Group, Boca Raton, FL, USA.