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2011 | 71 | S |
Tytuł artykułu

Hyperbaric preconditioning combined with post-ischemic hyperbaric therapy prevents ischemia-evoked damage to CA1 hippocampal neurons of Mongolian gerbils

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Języki publikacji
EN
Abstrakty
EN
Lack of the clinically effective pharmacological neuroprotection in different forms of brain ischemia increased the interest in alternative methods of therapy, like hypothermia or induction of brain tolerance by pre- and post-conditioning. The hyperbaric oxygen (HBO) therapy (2.5 atm) applied after ischemia or traumatic brain injury is one of the proposed but still controversial methods. The aim of this study was to find whether HBO and hyperbaric air (HBA) preconditioning followed by hyperbaric treatment applied for 60 min at different times after 3 min forebrain ischemia in gerbils will give a significant protection. The effects of both treatments on brain temperature and animal behaviour were also examined. A telemetric system to measure brain temperature was used and for behavioural observations a nest building test. The density of viable CA1 pyramidal neurons was also quantified. Our results show that HBO preconditioning combined with HBO postischemic therapy significantly reduced ischemia-evoked increase of brain temperature. HBA was also effective. Both treatments significantly increased gerbils’ ability to build a nest in comparison to untreated animals. The best effect was observed when postischemic therapy was applied 1 h after ischemia, but it was also effective 3 h after ischemia. Morphological analysis showed that HBO preconditioning combined with HBO postischemic treatment applied 1 h after ischemia significantly reduced neuronal damage in CA1 region of hippocampus resulting in 85% of surviving neurons compared to 18% of surviving CA1 neurons in the brains of animals subjected to ischemia but not treated with HBO. Our results show that HBO preconditioning combined with HBO therapy after forebrain ischemia in gerbils gives morphological protection which is accompanied by good behavioral results. Apart from inducing tolerance mediated by mild oxidative stress, HBO may affect blood oxygenation and other factors instrumental in brain protection.
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Wydawca
-
Rocznik
Tom
71
Numer
S
Opis fizyczny
p.73
Twórcy
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre PAS, Warsaw, Poland
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Typ dokumentu
Bibliografia
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