Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study

• Autorzy: Oguz A. , Boyuk A. , Ekinci A. , Alabalik U. , Turkoglu A. , Tuncer M.C. , Ekingen A. , Deveci E. , Gulturk B. , Aday U.
• Języki publikacji: EN

Abstrakty

EN

Background: The aim of the study was to investigate the protective effects of rosmarinic acid in rats exposed to hepatic ischaemia/reperfusion (I/R) injury. Materials and methods: Thirty-two rats were randomly classified into four groups of 8 rats each: laparotomy without medication, rosmarinic acid (dose of 50 mg/kg via oral gavage) followed by laparotomy, laparotomy followed by hepatic I/R, and hepatic I/R with rosmarinic acid. Serum aspartate aminotransferase, alanine aminotransferase, and malondialdehyde levels and total oxidant activity and total antioxidant capacity levels of the liver, lung, and kidney were assessed. The histopathologic assessment was also performed. Results: Rosmarinic acid significantly reduced liver function test parameters and decreased oxidative stress and abnormal histopathologic findings in the liver. The oxidative stress in the lung significantly increased in the I/R group but significantly decreased in the I/R + rosmarinic acid group due to the addition of rosmarinic acid. Rosmarinic acid led to no reduction in oxidative stress in kidney following hepatic I/R injury. There were no statistically significant differences among the groups regarding histopathologic changes in kidney and lung sections. Conclusions: Rosmarinic acid has antioxidant properties and is an effective hepatoprotective agent. However, although rosmarinic acid provides useful effects in the lung by increasing antioxidant capacity and reducing oxidative stress after I/R injury, it does not ameliorate histopathologic changes. These findings suggest that rosmarinic acid is likely to provide favourable outcomes in the treatment of hepatic I/R injury. (Folia Morphol 2020; 79, 2: 288–295)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2020
• Tom: 79
• Numer: 2
• Opis fizyczny: p.288-295,fig.,ref.

Twórcy

autor

Oguz A.

• Department of General Surgery, Faculty of Medicine, University of Dicle, Diyarbakır, Turkey

autor

Boyuk A.

• Department of General Surgery, University of Health Sciences Elazig Training and Research Hospital, Elazig, Turkey

autor

Ekinci A.

• Department of Biochemistry, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey

autor

Alabalik U.

• Department of Pathology, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey

autor

Turkoglu A.

• Department of General Surgery, Medical Park Gaziantep Hospital, Gaziantep, Turkey

autor

Tuncer M.C.

• Department of Anatomy, Faculty of Medicine, University of Dicle, Diyarbakir, Turkey

autor

Ekingen A.

• Vocational High School of Health Services, University of Batman, Baman, Turkey

autor

Deveci E.

• Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey

autor

Gulturk B.

• Department of General Surgery, Elazıg Fethi Sekin City Hospital, Elazıg, Turkey

autor

Aday U.

• Department of General Surgery, Elazıg Fethi Sekin City Hospital, Elazıg, Turkey

Bibliografia

• 1. Aktan B, Taysi S, Gumustekin K, et al. Evaluation of oxidative stress in erythrocytes of guinea pigs with experimental otitis media and effusion. Ann Clin Lab Sci. 2003; 33(2): 232–236, indexed in Pubmed: 12817629.
• 2. Arab HA, Sasani F, Rafiee MH, et al. Histological and biochemical alterations in early-stage lobar ischemia-reperfusion in rat liver. World J Gastroenterol. 2009; 15(16): 1951–1957, doi: 10.3748/wjg.15.1951, indexed in Pubmed: 19399926.
• 3. Bolukbas C, Bolukbas FF, Horoz M, et al. Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis. 2005; 5: 95, doi: 10.1186/1471-2334-5-95, indexed in Pubmed: 16262897.
• 4. Böyük A, Onder A, Kapan M, et al. Ellagic acid ameliorates lung injury after intestinal ischemia-reperfusion. Pharmacogn Mag. 2011; 7(27): 224–228, doi: 10.4103/0973-1296.84236, indexed in Pubmed: 21969793.
• 5. Chatterjee PK, Patel NSA, Kvale EO, et al. Inhibition of inducible nitric oxide synthase reduces renal ischemia/reperfusion injury. Kidney Int. 2002; 61(3): 862–871, doi: 10.1046/j.1523-1755.2002.00234.x, indexed in Pubmed: 11849439.
• 6. Choi HR, Choi JS, Han YN, et al. Peroxynitrite scavenging activity of herb extracts. Phytother Res. 2002; 16(4): 364–367, doi: 10.1002/ptr.904, indexed in Pubmed: 12112294.
• 7. Chu X, Ci X, He J, et al. Effects of a natural prolyl oligopeptidase inhibitor, rosmarinic acid, on lipopolysaccharide-induced acute lung injury in mice. Molecules. 2012; 17(3): 3586–3598, doi: 10.3390/molecules17033586, indexed in Pubmed: 22441336.
• 8. Domitrović R, Skoda M, Vasiljev Marchesi V, et al. Rosmarinic acid ameliorates acute liver damage and fibrogenesis in carbon tetrachloride-intoxicated mice. Food Chem Toxicol. 2013; 51: 370–378, doi: 10.1016/j.fct.2012.10.021, indexed in Pubmed: 23116643.
• 9. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12): 1103–1111, doi: 10.1016/j.clinbiochem.2005.08.008, indexed in Pubmed: 16214125.
• 10. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004; 37(2): 112–119, doi: 10.1016/j.clinbiochem.2003.10.014, indexed in Pubmed: 14725941.
• 11. Hammouda el-R A, Khalil MM, Salem A. Lipid peroxidation products in pleural fluid for separation of transudates and exudates. Clin Chem. 1995; 41(9): 1314–1315, doi: 10.1093/clinchem/41.9.1314.
• 12. Han S, Yang S, Cai Z, et al. Anti-Warburg effect of rosmarinic acid via miR-155 in gastric cancer cells. Drug Des Devel Ther. 2015; 9: 2695–2703, doi: 10.2147/DDDT.S82342, indexed in Pubmed: 26056431.
• 13. Jordán MJ, Lax V, Rota MC, et al. Relevance of carnosic acid, carnosol, and rosmarinic acid concentrations in the in vitro antioxidant and antimicrobial activities of Rosmarinus officinalis (L.) methanolic extracts. J Agric Food Chem. 2012; 60(38): 9603–9608, doi: 10.1021/jf302881t, indexed in Pubmed: 22957812.
• 14. Kapan M, Gumus M, Onder A, et al. The effects of ellagic acid on the liver and remote organs’ oxidative stress and structure after hepatic ischemia reperfusion injury caused by pringle maneuver in rats. Bratisl Lek Listy. 2012; 113(5): 274–281, doi: 10.4149/bll_2012_064, indexed in Pubmed: 22616584.
• 15. Kesik V, Guven A, Vurucu S, et al. Melatonin and 1400 W ameliorate both intestinal and remote organ injury following mesenteric ischemia/reperfusion. J Surg Res. 2009; 157(1): e97–e9e105, doi: 10.1016/j.jss.2008.12.024, indexed in Pubmed: 19394656.
• 16. Koksel O, Yildirim C, Cinel L, et al. Inhibition of poly(ADP-ribose) polymerase attenuates lung tissue damage after hind limb ischemia-reperfusion in rats. Pharmacol Res. 2005; 51(5): 453–462, doi: 10.1016/j.phrs.2004.11.007, indexed in Pubmed: 15749460.
• 17. Lee HJ, Cho HS, Park E, et al. Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced apoptosis. Toxicology. 2008; 250(2-3): 109–115, doi: 10.1016/j.tox.2008.06.010, indexed in Pubmed: 18644421.
• 18. Lee HT, Park SW, Kim M, et al. Acute kidney injury after hepatic ischemia and reperfusion injury in mice. Lab Invest. 2009; 89(2): 196–208, doi: 10.1038/labinvest.2008.124, indexed in Pubmed: 19079326.
• 19. Man K, Fan ST, Ng IO, et al. Prospective evaluation of Pringle maneuver in hepatectomy for liver tumors by a randomized study. Ann Surg. 1997; 226(6): 704–11; discussion 711, doi: 10.1097/00000658-199712000-00007, indexed in Pubmed: 9409569.
• 20. Mariani E, Cornacchiola V, Polidori MC, et al. Antioxidant enzyme activities in healthy old subjects: influence of age, gender and zinc status: results from the Zincage Project. Biogerontology. 2006; 7(5-6): 391–398, doi: 10.1007/s10522-006-9054-6, indexed in Pubmed: 16967205.
• 21. Miranda LE, Capellini VK, Reis GS, et al. Effects of partial liver ischemia followed by global liver reperfusion on the remote tissue expression of nitric oxide synthase: lungs and kidneys. Transplant Proc. 2010; 42(5): 1557–1562, doi: 10.1016/j.transproceed.2010.02.097, indexed in Pubmed: 20620474.
• 22. Moon DO, Kim MO, Lee JD, et al. Rosmarinic acid sensitizes cell death through suppression of TNF-alpha-induced NF-kappaB activation and ROS generation in human leukemia U937 cells. Cancer Lett. 2010; 288(2): 183–191, doi: 10.1016/j.canlet.2009.06.033, indexed in Pubmed: 19619938.
• 23. Nunes S, Madureira AR, Campos D, et al. Therapeutic and nutraceutical potential of rosmarinic acid-cytoprotective properties and pharmacokinetic profile. Crit Rev Food Sci Nutr. 2017; 57(9): 1799–1806, doi: 10.1080/10408398.2015.1006768, indexed in Pubmed: 26114303.
• 24. Oguz A, Kapan M, Onder A, et al. The effects of curcumin on the liver and remote organs after hepatic ischemia reperfusion injury formed with Pringle manoeuvre in rats. Eur Rev Med Pharmacol Sci. 2013; 17(4): 457–466, indexed in Pubmed: 23467943.
• 25. Osakabe N, Takano H, Sanbongi C, et al. Anti-inflammatory and anti-allergic effect of rosmarinic acid (RA); inhibition of seasonal allergic rhinoconjunctivitis (SAR) and its mechanism. Biofactors. 2004; 21(1-4): 127–131, doi: 10.1002/biof.552210125, indexed in Pubmed: 15630183.
• 26. Ota S, Nakamura K, Yazawa T, et al. High tidal volume ventilation induces lung injury after hepatic ischemia-reperfusion. Am J Physiol Lung Cell Mol Physiol. 2007; 292(3): L625–L631, doi: 10.1152/ajplung.00151.2006, indexed in Pubmed: 17056704.
• 27. Ozturk H, Ozturk H, Terzi EH, et al. Protective effects of rosmarinic acid against renal ischaemia/reperfusion injury in rats. J Pak Med Assoc. 2014; 64(3): 260–265, indexed in Pubmed: 24864596.
• 28. Park SW, Chen SWC, Kim M, et al. Human activated protein C attenuates both hepatic and renal injury caused by hepatic ischemia and reperfusion injury in mice. Kidney Int. 2009; 76(7): 739–750, doi: 10.1038/ki.2009.255, indexed in Pubmed: 19625989.
• 29. Ramalho LN, Pasta ÂA, Terra VA, et al. Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats. Food Chem Toxicol. 2014; 74: 270–278, doi: 10.1016/j.fct.2014.10.004, indexed in Pubmed: 25455894.
• 30. Rocha J, Eduardo-Figueira M, Barateiro A, et al. Anti-inflammatory effect of rosmarinic acid and an extract of Rosmarinus officinalis in rat models of local and systemic inflammation. Basic Clin Pharmacol Toxicol. 2015; 116(5): 398–413, doi: 10.1111/bcpt.12335, indexed in Pubmed: 25287116.
• 31. Sahu A, Rawal N, Pangburn MK. Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. Biochem Pharmacol. 1999; 57(12): 1439–1446, doi: 10.1016/s0006-2952(99)00044-1, indexed in Pubmed: 10353266.
• 32. Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia-reperfusion injury. Am J Surg. 2001; 181(2): 160–166, doi: 10.1016/s0002-9610(00)00573-0, indexed in Pubmed: 11425059.
• 33. Sevgi K, Tepe B, Sarikurkcu C. Antioxidant and DNA damage protection potentials of selected phenolic acids. Food Chem Toxicol. 2015; 77: 12–21, doi: 10.1016/j.fct.2014.12.006, indexed in Pubmed: 25542528.
• 34. Sotnikova R, Okruhlicova L, Vlkovicova J, et al. Rosmarinic acid administration attenuates diabetes-induced vascular dysfunction of the rat aorta. J Pharm Pharmacol. 2013; 65(5): 713–723, doi: 10.1111/jphp.12037, indexed in Pubmed: 23600389.
• 35. Sroka Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol. 2003; 41(6): 753–758, doi: 10.1016/s0278-6915(02)00329-0, indexed in Pubmed: 12738180.
• 36. Ulger BV, Erbis H, Turkcu G, et al. Nebivolol ameliorates hepatic ischemia/reperfusion injury on liver but not on distant organs. J Invest Surg. 2015; 28(5): 245–252, doi: 10.3109/08941939.2015.1031923, indexed in Pubmed: 26305470.
• 37. Xu Y, Jiang Z, Ji G, et al. Inhibition of bone metastasis from breast carcinoma by rosmarinic acid. Planta Med. 2010; 76(10): 956–962, doi: 10.1055/s-0029-1240893, indexed in Pubmed: 20157877.
• 38. Xu Y, Xu G, Liu Li, et al. Anti-invasion effect of rosmarinic acid via the extracellular signal-regulated kinase and oxidation-reduction pathway in Ls174-T cells. J Cell Biochem. 2010; 111(2): 370–379, doi: 10.1002/jcb.22708, indexed in Pubmed: 20506543.
• 39. Zimmerman BJ, Granger DN, Zimmerman BJ, et al. Reperfusion injury. Surg Clin North Am. 1992; 72(1): 65–83, doi: 10.1016/s0039-6109(16)45628-8, indexed in Pubmed: 1731390.

Identyfikatory

DOI

10.5603/FM.a2019.0087