Thioridazine induces erythrocyte stomatocytosis due to interactions with negatively charged lipids

• Autorzy: Hendrich A.B. , Lichacz K. , Burek A. , Michalak K.
• Języki publikacji: EN

Abstrakty

EN

Despite the fact that thioridazine is used clinically as a neuroleptic drug, little is known about the molecular mechanisms underlying its biological effects, in particular about its interactions with membranes. In the present work we investigate the influence of thioridazine on model and cell membranes, using calorimetry, DPH fluorescence polarization measurements, studies of haemolysis and scanning electron microscopy. The experiments show that thioridazine interacts with lipid bilayers and intercalates into bilayer structure. We found that erythrocyte stomatocytosis induced by the drug might be related to preferential interaction of thioridazine with charged lipids.

Słowa kluczowe

EN

thioridazine derivative; neuroleptic drug; erythrocyte; stomatocytosis; interaction; calorimetry; lipid bilayer

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 4
• Opis fizyczny: p.1081-1086,fig.,ref.

Twórcy

autor

Hendrich A.B.

• Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50–368 Wroclaw, Poland

autor

Lichacz K.

• Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50–368 Wroclaw, Poland

autor

Burek A.

• Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50–368 Wroclaw, Poland

autor

Michalak K.

• Department of Biophysics, Wroclaw Medical University, Chalubinskiego 10, 50–368 Wroclaw, Poland

Bibliografia

• 1. Saito, M., Iimori, Y., Kamoshita, S., Yanagisawa, M. and Sakakihara, Y. Effects of peroxisomal β-oxidation antagonist on 2',3'-cyclic-nucleotide 3'- phosphohydrolase, membrane lipid compositions, and membrane fluidity in C-6 glial cells. Biochim. Biophys. Acta 1235 (1995) 178-182.
• 2. Rodrigues, T., Santos, A. C., Pigoso, A. A., Mingatto, F. E., Uyemura, S. A. and Curti, C. Thioridazine interacts with the membrane of mitochondria acquiring antioxidant activity toward apoptosis - potentially implicated mechanisms. Br. J. Pharmacol. 136 (2002) 136-142.
• 3. Hendrich, A. B., Wesołowska, O. and Michalak, K. Trifluoperazine induces domain formation in zwitterionic phosphatidylcholine but not in charged phosphatidylglycerol bilayers. Biochim. Biophys. Acta 1510 (2001) 414-425.
• 4. Hendrich, A. B., Wesołowska, O., Komorowska, M., Motohashi, N. and Michalak, K. The alterations of lipid bilayer fluidity induced by newly synthesized phenothiazine derivative. Biophys. Chem. 98 (2002) 275-285.
• 5. Hägerstrand, H. and Isomaa, B. Morphological characterization of exovesicles and endovesicles released from human erythrocytes following treatment with amphiphiles. Biochim. Biophys. Acta 1109 (1992) 117-126.
• 6. Bobrowska-Hägerstrand, M., Kralj-Iglic, V., Iglic, A., Bialkowska, K., Isomaa, B. and Hägerstrand, H. Torocyte membrane endovesicles induced by octaethylenglycol dodecylether in human erythrocytes. Biophys. J. 77 (1999) 3356-3362.
• 7. Nerdal, W., Gundersen, S. A., Thorsen, V., Hoiland, H. and Holmsen, H. Chlorpromazine interaction with glycerophospholipid liposomes studied by magie angle spinning solid state 13C-NMR and differential scanning calorimetry Biochim. Biophys. Acta 1464 (2000) 165-175.
• 8. Moncelli, M. R. and Becucci, L. Effect of the molecular structure of more common amphiphilic molecules on their interactions with dioleoyl- phosphatidylcholine monolayers. Bioelectrochem. Bioenerg. 39 (1996) 227-234.
• 9. Deuticke, B. Transformation and restoration of biconcave shape of human erythrocytes induced by amphiphilic agents and change of ionic environment. Biochim. Biophys. Acta 163 (1968) 494-500.
• 10. Isomaa, B., Hagerstrand, H. and Paatero, G. Shape transformations induced by amphiphiles in erythrocytes. Biochim. Biophys. Acta 899 (1987) 93-103.
• 11. Schrier, S. L., Zachowski, A. and Devaux, P. F. Mechanisms of amphipath-induced stomatocytosis in human erythrocytes. Blood 79 (1992) 782-786.
• 12. Sheetz, M. P. and Singer, S. J. Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions. Proc. Natl. Acad. Sci. USA 71 (1974) 4457-4461.
• 13. Castaing, M., Brouant, P., Loiseau, A., Santelli-Rouvier, C., Santelli, M., Alibert-Franco, S., Mahamoud, A. and Barbe, J. Membrane permeation by multidrug-resistance-modulators and non-modulators: effects of hydrophobicity and electric charge. J. Pharm. Pharmacol. 52 (2000) 289-296.
• 14. Sikorski, A. F., Hanus-Lorenz, B., Jezierski, A. and Dłużewski, A. R. Interaction of membrane skeletal proteins with membrane lipid domain. Acta Biochim. Pol. 47 (2000) 565-578.
• 15. Minetti, M. and Di Stasi, A. M. M. Involvement of erythrocyte skeletal proteins in the modulation of membrane fluidity by phenothiazines. Biochemistry 26 (1987) 8133-8137.