Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity

• Autorzy: Kumar V. , Sharma A. , Narasimha Prasad B. C. , Gururaj H. B. , Giridhar P. , Ravishankar G. A.
• Języki publikacji: EN

Abstrakty

EN

Direct shoot bud induction and plant regeneration was achieved in Capsicum frutescens var. KTOC. Aseptically grown seedling explants devoid of roots, apical meristem and cotyledons were inoculated in an inverted position in medium comprising of Murashige and Skoog (Physiol Plant 15:472–497, 1962) basal medium supplemented with 2-(N-morpholine) ethanesulphonic acid buffer along with 2.28 µM indole-3-acetic acid, 10 µM silver nitrate and either of 13.31– 89.77 µM benzyl adenine (BA), 9.29–23.23 µM kinetin, 0.91–9.12 µM zeatin, 2.46–9.84 µM 2-isopentenyl adenine. Profuse shoot bud induction was observed only in explants grown on a media supplemented with BA (26.63 µM) as a cytokinin source and 19.4 ± 4.2 shoot buds per explant was obtained in inverted mode under continuous light. Incorporation of polyamine inhibitors in the culture medium completely inhibited shoothoot bud induction. Incorporation of exogenous polyamines improved the induction of shoot buds under 24 h photoperiod. These buds were elongated in MS medium containing 2.8 µM gibberellic acid. Transfer of these shoots to hormone-free MS medium resulted in rooting and rooted plants were transferred to fields. This protocol can be efficiently used for mass propagation and presumably also for regeneration of genetically transformed C. frutescens.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2007
• Tom: 29
• Numer: 1
• Opis fizyczny: p.11-18,fig.,ref.

Twórcy

autor

Kumar V.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

autor

Sharma A.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

autor

Narasimha Prasad B. C.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

autor

Gururaj H. B.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

autor

Giridhar P.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

autor

Ravishankar G. A.

• Central Food Technological Research Institute, Plant Cell Biotechnology Department, Mysore 570 020 Karnataka, India

Bibliografia

• Apelbaum A, Goldlust A, Icekson I (1985) Control of ethylene by arginine decarboxylase activity in pea seedlings and its implication for hormonal regulation of plant growth. Plant Physiol 79:635
• Bais HP, Ravishankar GA (2002) Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Cult 69:1–34
• Bais HP, Sudha GS, Ravishankar GA (2000) Putrescine and AgNO₃ influences shoot multiplication in vitro flowering and endogenous titers of polyamines in Cichorium intybus L cv Lucknow local. J Plant Growth Regul 19:238–248
• Bais HP, Sudha GS, Ravishankar GA (2001) Influence of putrescine AgNO₃ and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cichorium intybus and their regenerants. Plant Cell Rep 20:547–555
• Beyer EM (1976) A potent inhibitor of ethylene action in plants. Plant Physiol 58:268–271
• Binzel ML, Sankhla N, Joshi S, Sankhla D (1996) Induction of somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.). Plant Cell Rep 15:536–540
• Chi CL, Pua EC (1989) Ethylene inhibitor enhanced de novo shoot regeneration from cotyledons of B. campestris spp. chinensis (Chinese cabbage) in vitro. Plant Sci 64:240–243
• Chi CL, Lin WS, Lee JEE, Pua EC (1994) Role of polyamines on de novo shoot morphogenesis from cotyledons of B. campestris spp. pekinesis (lour) olsson, in vitro. Plant Cell Rep 13:323–329
• Christopher T, Rajam MV (1994) In vitro clonal propagation of Capsicum spp. Plant Cell Tissue Organ Cult 38:25–29
• Davies PJ (1987) The plant hormones: their nature, occurrence, and functions. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Martinus Nijhoff, Boston
• Ebida AIA, Hu CY (1993) In vitro morphogenetic responses and plant regeneration from pepper (Capsicum annuum L. Cv Early California Wonder) seedling explants. Plant Cell Rep 13:107–110
• Evans PT, Malmberg RL (1989) Do polyamines have role in plant development? Annu Rev Plant Physiol Plant Mol Biol 40:235–269
• Feirer RP, Mignon G, Litvay JD (1984) Arginine decarboxylase and polyamines required for embryogenesis in wild carrot. Science 223:1433–1435
• Galston AW (1983) Polyamines as modulators of plant development. Bioscience 33:382–388
• Govindarajan VS (1985) Capsicum—production, technology, chemistry and quality—part II, processed products, standards, world production and trade. CRC Crit Rev Nutr 23:207
• Gururaj HB, Giridhar P, Sharma A, Prasad BCN, Ravishankar GA (2004) In vitro clonal propagation of bird eye chilli (Capsicum frutescens Mill.). Indian J Exp Biol 42(11):1136–1140
• Harini I, Lakshmisita G (1993) Direct somatic embryogenesis and plant regeneration from immature embryos of chilli (Capsicum annuum L.). Plant Sci 89:107–112
• Husain S, Jain A, Kothari SL (1999) Phenyl acetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. Plant Cell Rep 19:64–68
• Hyde CL, Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of chili pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell Dev Biol Plant 32:72–80
• Kim S, Kim SR, An CS, Hong YN, Lee KW (2001) Constitutive expression of rice MADS box gene using seed explants in hot pepper (Capsicum annuum L.). Mol Cells 12:221–226
• Kumar V, Gururaj HB, Prasad BCN, Giridhar P, Ravishankar GA (2005) Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L. Sci Hortic 106:237–246
• Liu W, Parrot WA, Hilderbrand DF, Collins GB, Williams EG (1990) Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot like structures expressing introduced genes. Plant Cell Rep 9:360–364
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:472–497
• Ochoa-Alejo N, Ramirez-Malagon R (2001) In vitro chilli pepper biotechnology. In Vitro Cell Dev Biol Plant 37:701–729
• Phillips GC, Hubstenberger JF (1985) Organogenesis in pepper tissue cultures. Plant Cell Tissue Organ Cult 4:261–269
• Pua CE, Sim EG, Chi LG, Kong FL (1996) Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyls explants of Chinese radish (Raphanus sativus L var longipinnatus Bailey) in vitro. Plant Cell Rep 15:685–690
• Reddy OB, Giridhar P, Ravishankar GA (2002) The effect of triacontanol on micropropagation of Capsicum frutescens L. and Decalepis hamiltonii W&A. Plant Cell Tissue Organ Cult 71(3):253–258
• Roustan JP, Latche A, Fallot J (1990) Control of carrot somatic embryogenesis by AgNO₃ an inhibitor of ethylene action effect on arginine decarboxylase activity. Plant Sci 67:89–95
• Smith TA (1985) Plant polyamines metabolism and function polyamine synthesis. In: Flores HE, Arteca RN, Shannon JC (eds) Polyamines and ethylene: biochemistry, physiology and interaction. American Society of Plant Physiologists, Rockville, pp 1–23
• Steinitz B, Kusek S, Tabib Y, Paran I, Zelcer A (2003) Pepper (Capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shoot. In Vitro Cell Dev Biol Plant 39:296–303
• Sushma T, Palni LMS (2004) Studies on in vitro propagation of Himalayan ceder (Cedrus deodara) using zygotic embryos and stem segments. Indian J Biotech 3:209–215
• Valera-Montero LL, Ochoa-Alejo N (1992) A novel approach for chili pepper (Capsicum annuum L.) plant regeneration: shoot induction in rooted hypocotyls. Plant Sci 84:215–219
• Zhu YX, On-Yang WJ, Zang YF, Chen ZL (1996) Transgenic sweet pepper plants from Agrobacterium mediated transformation. Plant Cell Rep 16:71–75