Molecular survey of parasites in introduced Pelophylax perezi (Ranidae) water frogs in the Azores

• Autorzy: Harris D.J. , Spigonardi M. P. , Maia J. P. M. C. , Cunha R. T.
• Języki publikacji: EN

Abstrakty

EN

Water frogs, Pelophylax perezi, that are introduced in the Azores, were screened for parasites using PCR primers known to amplify Apicomplexa parasites, and using nematode-specific primers. With the former, three different organisms were detected: Hepatozoon, a trichodinid protozoan ciliate and a possible Stramenopile. Using the latter set of primers, a single unknown spirurid nematode was also detected. Phylogenetic analyses indicate that Hepatozoon detected within amphibian hosts appear to form a clade, although relationships of these parasites do not match the vertebrate intermediate host phylogeny. Regarding the possible Stramenopile, it is unclear whether this organism was actually present on the amphibian or in the water on the surface of the tissue sample. Our findings highlight that many different organisms can be detected with these primers and that they can be used to screen introduced host populations to detect parasites that have been brought with them.

Słowa kluczowe

EN

molecular survey; parasite; Pelophylax perezi; water frog; Azores Islands; Amphibia; Hepatozoon; Trichodina; Eimeriidae; nematode; Apicomplexa; 18S rRNA gene

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 4
• Opis fizyczny: p.607-611,fig.,ref.

Twórcy

autor

Harris D.J.

• Centro de Investigaçao em Biodiversidade e Recursos Geneticos da Universidade do Porto, Campus Agrario de Vairao, 4485-661 Vairao, Portugal

autor

Spigonardi M. P.

• Centro de Investigaçao em Biodiversidade e Recursos Geneticos da Universidade do Porto, Campus Agrario de Vairao, 4485-661 Vairao, Portugal

autor

Maia J. P. M. C.

• Centro de Investigaçao em Biodiversidade e Recursos Geneticos da Universidade do Porto, Campus Agrario de Vairao, 4485-661 Vairao, Portugal

autor

Cunha R. T.

• Centro de Investigacao em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado, Polo dos Acores, Universidade dos Acores, 9501-801 Ponta Delgada, Portugal

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