The effect of Nicotiana tabacum L. extracts on cultures of tobacco callus

• Autorzy: Hanus-Fajerska E. , Ciarkowska K.

Warianty tytułu

PL

Wpływ ekstraktów Nicotiana tabacum L. na kultury kalusa tytoniu

• Języki publikacji: EN

Abstrakty

EN

Solanaceae plants are a source of large number of valuable metabolites with multiple use both in vivo and in vitro conditions. Leaf explants obtained from N. tabacum ‘Samsun’ were cultivated on solidified MS medium supplemented with 3% (w/v) sucrose and enriched with different doses of extracts obtained from seedlings of the same plant cultivar or from N. tabacum ‘Havana’. The aim of experiments was to verify the effectiveness of such medium additives on callus tissue vitality, proliferation and its organogenetic response. It was ascertained a significant positive influence of all kinds of added extracts on increase of callus fresh weight and the organogenetic capability of tobacco cultures. However, the impact of seedlings devoid of roots Nicotiana extracts was significantly lower in both: stimulating of cell proliferation and shoot formation than extracts obtained from whole seedlings. The biological activity was the most effective in the case of genotype compatibility between the plant material from which the extract was derived with the object of biotest.

PL

Rożliny psiankowate stanowią Żródło cennych metabolitów o wielorakim zastosowaniu zarówno in vivo, jak i in vitro. Eksplantaty liściowe izolowane z N. tabacum ‘Samsun’ kultywowano na zestalonej pożywce MS z dodatkiem 3% sacharozy, wzbogaconej zróżnicowanymi dawkami ekstraktów uzyskanych z siewek analogicznej odmiany tytoniu lub z odmiany N. tabacum ‘Havana’. Celem przeprowadzonych badań była ocena skuteczności wpływu takich ekstraktów na żywotność i proliferację oraz na odpowiedź morfogenetyczną kultur kalusa. Stwierdzono pozytywne działanie zastosowanych ekstraktów zarówno na wzrost świeżej masy kalusa, jak i na potencjał morfogenetyczny kultur tytoniu. Wyciąg uzyskany z siewek pozbawionych korzeni działał mniej skutecznie, zarówno na tempo proliferacji komórek, jak i na kaulogenezę, w porównaniu z otrzymanym z całych siewek. Największa aktywność biologiczna ekstraktów została stwierdzona w przypadku zgodności genotypowej Żródła ekstraktu i tkanek zastosowanych jako biotest.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 4
• Opis fizyczny: p.59-66,fig.,ref.

Twórcy

autor

Hanus-Fajerska E.

• Department of Botany and Plant Physiology, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Krakow, Poland

autor

Ciarkowska K.

• Department of Soil Science and Soil Protection, University of Agriculture in Krakow, Mickiewicza 21, 31-120 Krakow, Poland

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