High concordance of Pfdhfr and Pfdhps genotypes between matched peripheral and placental isolates of delivered women in Bobo-Dioulasso, Burkina Faso

• Autorzy: Cisse M. , Awandare G.A. , Some F.A. , Hayette M.-P. , Guiguemde R.T.
• Języki publikacji: EN

Abstrakty

EN

Whether maternal peripheral parasites constitute a representative sample of the overall population infecting the individual, remains unknown in Burkina Faso. We therefore compared Pfdhfr and Pfdhps genotypes between matched peripheral and placental isolates. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of polymorphic codons of the Pfdhfr gene (51, 59, 108 and 164) and the Pfdhps gene (437 and 540) was performed in 18 matched peripheral and placental dried blood spots of delivered women in Bobo-Dioulasso. Both Pfdhfr and Pfdhps genes were successfully genotyped in 94.4% (17/18) of the matched samples. Only 8.8% (3/34) of genotypes were of the wild type, while 20.6% (7/34), 20.6% (7/34), 23.5% (8/34) and 26.5% (9/34) comprised one, two, three and four mutations, respectively. None of the samples carried both Pfdhfr I164L and Pfdhps K540E mutations. A concordance of 82.4% was observed in matched samples for both the Pfdhfr and Pfdhps genes. Setting placental alleles as the reference, a concordance of 100% was obtained with Pfdhfr mutation S108N, Pfdhfr mutation C59R+S108N, and Pfdhfr mutation N51I+C59R +S108N, respectively. Likewise, a concordance of 85.7% was observed with the Pfdhps mutation A437G. For epidemiological purposes, peripheral blood Pfdhfr and Pfdhps genotyping is sufficient for monitoring SP resistant molecular markers in pregnant women.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2017
• Tom: 63
• Numer: 2
• Opis fizyczny: p.111-116,ref.

Twórcy

autor

Cisse M.

• Laboratory of Parasitology and Entomology, Centre MURAZ, Bobo-Dioulasso, Burkina Faso
• Laboratory of Parasitology and Mycology, Universite Polytechnique de Bobo-Dioulasso, Bobo-Dioulasso, Burkina Faso

autor

Awandare G.A.

• College of Basic and Applied Sciences, West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Legon, Acra, Ghana

autor

Some F.A.

• Department of Parasitology, Institut de Recherche en Sciences de la Sante, Bobo-Dioulasso, Bobo-Dioulasso, Burkina Faso

autor

Hayette M.-P.

• Laboratory of Clinical Microbiology, University Hospital of Liege, Liege, Belgium

autor

Guiguemde R.T.

• Laboratory of Parasitology and Entomology, Centre MURAZ, Bobo-Dioulasso, Burkina Faso
• Laboratory of Parasitology and Mycology, Universite Polytechnique de Bobo-Dioulasso, Bobo-Dioulasso, Burkina Faso

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Identyfikatory

DOI

10.17420/ap6302.93